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- W3109210551 abstract "Demand for the detection of estradiol, which is a naturally occurring hormone, has been increasing. Gold nanoparticle-based colorimetric aptasensors have been developed for estradiol detection; however, the long sequence of aptamers due to the formation of the secondary structure likely affects the sensitivity of the aptasensors. Herein, a sensitive colorimetric biosensor is developed for label-free detection of estradiol by using an estradiol-specific split aptamer. The results demonstrate that a superior response is observed when a split aptamer with a high free energy of the secondary structure (ΔG > –3 kcal/mol) is used, in comparison to that observed using a split aptamer with a low free energy of the secondary structure (ΔG < –3 kcal/mol) at 27°C. After selecting the appropriate split aptamer, the standard calibration curve obtained for estradiol has a detection limit of 6.7 nM, with a linear range of 6.7 nM – 66.7 μM in the logarithmic scale. Furthermore, this assay is sensitive, easy-to-operate, inexpensive, and non-time-consuming (provides results within 50 min), thereby showing potential for clinical applications (detection of other small molecular targets)." @default.
- W3109210551 created "2020-12-07" @default.
- W3109210551 creator A5081305956 @default.
- W3109210551 creator A5088224159 @default.
- W3109210551 date "2020-12-04" @default.
- W3109210551 modified "2023-10-06" @default.
- W3109210551 title "Using Simple-Structured Split Aptamer for Gold Nanoparticle-based Colorimetric Detection of Estradiol" @default.
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- W3109210551 doi "https://doi.org/10.2116/analsci.20scp07" @default.
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