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• W3110053569 abstract "Abstract Bacterial cellulose (BC) has excellent material properties and can be produced cheaply and sustainably through simple bacterial culture, but BC-producing bacteria lack the extensive genetic toolkits of model organisms such as Escherichia coli . Here, we describe a simple approach for producing highly programmable BC materials through incorporation of engineered E. coli . The acetic acid bacterium Gluconacetobacter hansenii was co-cultured with engineered E. coli in droplets of glucose-rich media to produce robust cellulose capsules, which were then colonized by the E. coli upon transfer to selective lysogeny broth media. We show that the encapsulated E. coli can produce engineered protein nanofibers within the cellulose matrix, yielding hybrid capsules capable of sequestering specific biomolecules from the environment and enzymatic catalysis. Furthermore, we produced capsules capable of altering their own bulk physical properties through enzyme-induced biomineralization. This novel system, based on autonomous biological fabrication, significantly expands the functionality of BC-based living materials." @default.
• W3110053569 created "2020-12-07" @default.
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• W3110053569 date "2020-11-24" @default.
• W3110053569 modified "2023-10-16" @default.
• W3110053569 title "Hybrid Living Capsules Autonomously Produced by Engineered Bacteria" @default.
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• W3110053569 doi "https://doi.org/10.1101/2020.11.23.394965" @default.
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