FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3110269597> ?p ?o ?g. }

• W3110269597 endingPage "e0008308" @default.
• W3110269597 startingPage "e0008308" @default.
• W3110269597 abstract "Human African Trypanosomiasis (HAT) is a potentially fatal parasitic infection caused by the trypanosome sub-species Trypanosoma brucei gambiense and T . b . rhodesiense transmitted by tsetse flies. Currently, global HAT case numbers are reaching less than 1 case per 10,000 people in many disease foci. As such, there is a need for simple screening tools and strategies to replace active screening of the human population which can be maintained post-elimination for Gambian HAT and long-term for Rhodesian HAT. Here, we describe the proof of principle application of a novel high-resolution melt assay for the xenomonitoring of Trypanosoma brucei gambiense and T . b . rhodesiense in tsetse. Both novel and previously described primers which target species-specific single copy genes were used as part of a multiplex qPCR. An additional primer set was included in the multiplex to determine if samples had sufficient genomic material for detecting genes present in low copy number. The assay was evaluated on 96 wild-caught tsetse previously identified to be positive for T . brucei s . l . of which two were known to be positive for T . b . rhodesiense . The assay was found to be highly specific with no cross-reactivity with non-target trypanosome species and the assay limit of detection was 10 4 tryps/mL. The qPCR successfully identified three T . b . rhodesiense positive flies, in agreement with the reference species-specific PCRs. This assay provides an alternative to running multiple PCRs when screening for pathogenic sub-species of T . brucei s . l . and produces results in less than 2 hours, avoiding gel electrophoresis and subjective analysis. This method could provide a component of a simple and efficient method of screening large numbers of tsetse flies in known HAT foci or in areas at risk of recrudescence or threatened by the changing distribution of both forms of HAT." @default.
• W3110269597 created "2020-12-07" @default.
• W3110269597 creator A5014702689 @default.
• W3110269597 creator A5050738946 @default.
• W3110269597 creator A5057699085 @default.
• W3110269597 creator A5061910026 @default.
• W3110269597 creator A5062623523 @default.
• W3110269597 creator A5080754563 @default.
• W3110269597 date "2020-11-25" @default.
• W3110269597 modified "2023-09-27" @default.
• W3110269597 title "A pilot study demonstrating the identification of Trypanosoma brucei gambiense and T. b. rhodesiense in vectors using a multiplexed high-resolution melt qPCR" @default.
• W3110269597 cites W1966220751 @default.
• W3110269597 cites W1967477545 @default.
• W3110269597 cites W1973133877 @default.
• W3110269597 cites W1986742049 @default.
• W3110269597 cites W1988846622 @default.
• W3110269597 cites W1999989347 @default.
• W3110269597 cites W2003978461 @default.
• W3110269597 cites W2016085620 @default.
• W3110269597 cites W2030586191 @default.
• W3110269597 cites W2048434545 @default.
• W3110269597 cites W2053506129 @default.
• W3110269597 cites W2066841717 @default.
• W3110269597 cites W2073867453 @default.
• W3110269597 cites W2074584391 @default.
• W3110269597 cites W2100982533 @default.
• W3110269597 cites W2103226587 @default.
• W3110269597 cites W2117078561 @default.
• W3110269597 cites W2119067447 @default.
• W3110269597 cites W2123459894 @default.
• W3110269597 cites W2133545662 @default.
• W3110269597 cites W2139684763 @default.
• W3110269597 cites W2146578104 @default.
• W3110269597 cites W2146924802 @default.
• W3110269597 cites W2151362707 @default.
• W3110269597 cites W2155840678 @default.
• W3110269597 cites W2157856626 @default.
• W3110269597 cites W2315678501 @default.
• W3110269597 cites W2548762493 @default.
• W3110269597 cites W2742116434 @default.
• W3110269597 cites W2765828997 @default.
• W3110269597 cites W2772396595 @default.
• W3110269597 cites W2896846481 @default.
• W3110269597 cites W2942333799 @default.
• W3110269597 cites W3014522156 @default.
• W3110269597 cites W3016516499 @default.
• W3110269597 cites W4297644014 @default.
• W3110269597 doi "https://doi.org/10.1371/journal.pntd.0008308" @default.
• W3110269597 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7725321" @default.
• W3110269597 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33237917" @default.
• W3110269597 hasPublicationYear "2020" @default.
• W3110269597 type Work @default.
• W3110269597 sameAs 3110269597 @default.
• W3110269597 citedByCount "1" @default.
• W3110269597 countsByYear W31102695972022 @default.
• W3110269597 crossrefType "journal-article" @default.
• W3110269597 hasAuthorship W3110269597A5014702689 @default.
• W3110269597 hasAuthorship W3110269597A5050738946 @default.
• W3110269597 hasAuthorship W3110269597A5057699085 @default.
• W3110269597 hasAuthorship W3110269597A5061910026 @default.
• W3110269597 hasAuthorship W3110269597A5062623523 @default.
• W3110269597 hasAuthorship W3110269597A5080754563 @default.
• W3110269597 hasBestOaLocation W31102695971 @default.
• W3110269597 hasConcept C104317684 @default.
• W3110269597 hasConcept C153911025 @default.
• W3110269597 hasConcept C159047783 @default.
• W3110269597 hasConcept C2777459041 @default.
• W3110269597 hasConcept C2778143080 @default.
• W3110269597 hasConcept C2778792689 @default.
• W3110269597 hasConcept C2779502636 @default.
• W3110269597 hasConcept C2779549974 @default.
• W3110269597 hasConcept C2781188995 @default.
• W3110269597 hasConcept C2908647359 @default.
• W3110269597 hasConcept C2909346784 @default.
• W3110269597 hasConcept C49105822 @default.
• W3110269597 hasConcept C54355233 @default.
• W3110269597 hasConcept C71924100 @default.
• W3110269597 hasConcept C86803240 @default.
• W3110269597 hasConcept C90583042 @default.
• W3110269597 hasConcept C99454951 @default.
• W3110269597 hasConceptScore W3110269597C104317684 @default.
• W3110269597 hasConceptScore W3110269597C153911025 @default.
• W3110269597 hasConceptScore W3110269597C159047783 @default.
• W3110269597 hasConceptScore W3110269597C2777459041 @default.
• W3110269597 hasConceptScore W3110269597C2778143080 @default.
• W3110269597 hasConceptScore W3110269597C2778792689 @default.
• W3110269597 hasConceptScore W3110269597C2779502636 @default.
• W3110269597 hasConceptScore W3110269597C2779549974 @default.
• W3110269597 hasConceptScore W3110269597C2781188995 @default.
• W3110269597 hasConceptScore W3110269597C2908647359 @default.
• W3110269597 hasConceptScore W3110269597C2909346784 @default.
• W3110269597 hasConceptScore W3110269597C49105822 @default.
• W3110269597 hasConceptScore W3110269597C54355233 @default.
• W3110269597 hasConceptScore W3110269597C71924100 @default.
• W3110269597 hasConceptScore W3110269597C86803240 @default.
• W3110269597 hasConceptScore W3110269597C90583042 @default.
• W3110269597 hasConceptScore W3110269597C99454951 @default.
• W3110269597 hasFunder F4320334626 @default.

• next