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- W3110316222 abstract "Although the osteogenic differentiation potential of mesenchymal stem cells of dental origin is well established, the roles of different marker proteins in this process remain to be clarified. Our aim was to compare the cellular and molecular changes, focusing in particular on mesenchymal stem cell markers, during in vitro osteogenesis in three dental stem cell types: dental follicle stem cells (DFSCs), periodontal ligament stem cells (PDLSCs) and dental pulp stem cells (DPSCs). Human DFSCs, PDLSCs and DPSCs were isolated, cultured and their osteogenic differentiation was induced for 3 weeks. Mineralization was assessed by von Kossa staining and calcium concentration measurements. The expression of mesenchymal and osteogenic markers was studied by immunocytochemistry and qPCR techniques. Alkaline phosphatase (ALP) activity and the frequency of STRO-1 positive cells were also quantified. The three cultures all showed abundant mineralization, with high calcium content by day 21. The expression of vimentin and nestin was sustained after osteogenic induction. The osteogenic medium induced a considerable elevation of STRO-1 positive cells. By day 7, the ALP mRNA level had increased more than 100-fold in DFSCs, PDLSCs, and DPSCs. Quantitative PCR results indicated dissimilarities of osteoblastic marker levels in the three dental stem cell cultures. DFSCs, PDLSCs and DPSCs have similar functional osteogenic differentiation capacities although their expressional profiles of key osteogenic markers show considerable variations. The STRO-1 positive cell fraction expands during osteogenic differentiation while vimentin and nestin expression remain high. For identification of stemness, functional studies rather than marker expressions are needed." @default.
- W3110316222 created "2020-12-07" @default.
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- W3110316222 date "2021-02-01" @default.
- W3110316222 modified "2023-09-26" @default.
- W3110316222 title "STRO-1 positive cell expansion during osteogenic differentiation: A comparative study of three mesenchymal stem cell types of dental origin" @default.
- W3110316222 cites W1555330200 @default.
- W3110316222 cites W1574963476 @default.
- W3110316222 cites W1733152064 @default.
- W3110316222 cites W1969739545 @default.
- W3110316222 cites W1971205126 @default.
- W3110316222 cites W1982289725 @default.
- W3110316222 cites W1984859290 @default.
- W3110316222 cites W1986769065 @default.
- W3110316222 cites W1987145582 @default.
- W3110316222 cites W1999301154 @default.
- W3110316222 cites W2004152316 @default.
- W3110316222 cites W2005765776 @default.
- W3110316222 cites W2008362502 @default.
- W3110316222 cites W2008510527 @default.
- W3110316222 cites W2009308760 @default.
- W3110316222 cites W2010727648 @default.
- W3110316222 cites W2013976086 @default.
- W3110316222 cites W2015725078 @default.
- W3110316222 cites W2021190635 @default.
- W3110316222 cites W2024579131 @default.
- W3110316222 cites W2025371271 @default.
- W3110316222 cites W2025390380 @default.
- W3110316222 cites W2026048262 @default.
- W3110316222 cites W2027012204 @default.
- W3110316222 cites W2030670801 @default.
- W3110316222 cites W2031484018 @default.
- W3110316222 cites W2033524865 @default.
- W3110316222 cites W2034381565 @default.
- W3110316222 cites W2039322645 @default.
- W3110316222 cites W2064369032 @default.
- W3110316222 cites W2069987530 @default.
- W3110316222 cites W2083583313 @default.
- W3110316222 cites W2084436684 @default.
- W3110316222 cites W2086035803 @default.
- W3110316222 cites W2086982863 @default.
- W3110316222 cites W2087603582 @default.
- W3110316222 cites W2099947475 @default.
- W3110316222 cites W2106413293 @default.
- W3110316222 cites W2111379884 @default.
- W3110316222 cites W2112208102 @default.
- W3110316222 cites W2112626453 @default.
- W3110316222 cites W2115105861 @default.
- W3110316222 cites W2116864112 @default.
- W3110316222 cites W2117521358 @default.
- W3110316222 cites W2130516299 @default.
- W3110316222 cites W2142165217 @default.
- W3110316222 cites W2144224215 @default.
- W3110316222 cites W2145040826 @default.
- W3110316222 cites W2148562877 @default.
- W3110316222 cites W2149709195 @default.
- W3110316222 cites W2161351679 @default.
- W3110316222 cites W2162453787 @default.
- W3110316222 cites W2163106750 @default.
- W3110316222 cites W2328921773 @default.
- W3110316222 cites W2339683466 @default.
- W3110316222 cites W2415830104 @default.
- W3110316222 cites W2475771913 @default.
- W3110316222 cites W2514919627 @default.
- W3110316222 cites W2537492237 @default.
- W3110316222 cites W2541192728 @default.
- W3110316222 cites W2620407926 @default.
- W3110316222 cites W2765723427 @default.
- W3110316222 cites W2799666301 @default.
- W3110316222 cites W2806546824 @default.
- W3110316222 cites W2883292811 @default.
- W3110316222 cites W2889835308 @default.
- W3110316222 cites W2898640611 @default.
- W3110316222 cites W2899167227 @default.
- W3110316222 cites W2900516297 @default.
- W3110316222 cites W2919773296 @default.
- W3110316222 cites W2920839176 @default.
- W3110316222 cites W2933836641 @default.
- W3110316222 cites W2953304103 @default.
- W3110316222 doi "https://doi.org/10.1016/j.archoralbio.2020.104995" @default.
- W3110316222 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33278647" @default.
- W3110316222 hasPublicationYear "2021" @default.
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