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- W3111286738 abstract "Summary Piwi proteins use PIWI-interacting RNAs (piRNAs) to identify and silence the transposable elements (TEs) pervasively found in animal genomes. The Piwi targeting mechanism is proposed to be similar to targeting by Argonaute proteins, which employ microRNA (miRNA) guides to repress cellular mRNAs, but has not been characterized in detail. We present cryo-EM structures of a Piwi-piRNA complex with and without target RNAs and analysis of target recognition. Resembling Argonaute, Piwi identifies targets using the piRNA seed-region. However, Piwi creates a much weaker seed so that prolonged target association requires further piRNA-target pairing. Beyond the seed, Piwi creates wide central cleft wide for unencumbered piRNA-target pairing, enabling long-lived Piwi-piRNA-target interactions that are tolerant of mismatches. Piwi ensures targeting fidelity by blocking propagation of the piRNA-target duplex in the absence of faithful seed pairing, and by requiring extended piRNA-target pairing to reach an endonucleolytically active conformation. This mechanism allows Piwi to minimize off-targeting cellular mRNAs and adapt piRNA sequences to evolving genomic threats." @default.
- W3111286738 created "2020-12-21" @default.
- W3111286738 creator A5008393177 @default.
- W3111286738 creator A5020651944 @default.
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- W3111286738 creator A5072182664 @default.
- W3111286738 creator A5075736217 @default.
- W3111286738 creator A5076129805 @default.
- W3111286738 date "2020-12-07" @default.
- W3111286738 modified "2023-10-16" @default.
- W3111286738 title "Structural basis for piRNA-targeting" @default.
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