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- W3111482049 abstract "Abstract During the first steps of HIV infection the Env subunit gp41 is thought to establish contact between the membranes and to be the main driver of fusion. Here we investigated in liquid crystalline membranes the structure and cholesterol recognition of constructs made of a gp41 external region carrying a cholesterol recognition amino acid consensus (CRAC) motif and a hydrophobic membrane anchoring sequence. CD- und ATR-FTIR spectroscopies indicate that the constructs adopt a high degree of helical secondary structure in membrane environments. Furthermore, 15 N and 2 H solid-state NMR spectra of gp41 polypeptides reconstituted into uniaxially oriented bilayers agree with the CRAC domain being an extension of the transmembrane helix. Upon addition of cholesterol the CRAC NMR spectra remain largely unaffected when being associated with the native gp41 transmembrane sequence but its topology changes when anchored in the membrane by a hydrophobic model sequence. The 2 H solid-state NMR spectra of deuterated cholesterol are indicative of a stronger influence of the model sequence on this lipid when compared to the native gp41 sequence. These observations are suggestive of a strong coupling between the transmembrane and the membrane proximal region of gp41 possibly enforced by oligomerization of the transmembrane helical region." @default.
- W3111482049 created "2020-12-21" @default.
- W3111482049 creator A5001033334 @default.
- W3111482049 creator A5058017842 @default.
- W3111482049 creator A5061874808 @default.
- W3111482049 date "2020-12-17" @default.
- W3111482049 modified "2023-10-13" @default.
- W3111482049 title "Structure, membrane topology and influence of cholesterol of the membrane proximal region: transmembrane helical anchor sequence of gp41 from HIV" @default.
- W3111482049 cites W1493154674 @default.
- W3111482049 cites W1519704494 @default.
- W3111482049 cites W1528872128 @default.
- W3111482049 cites W1635348995 @default.
- W3111482049 cites W1965151399 @default.
- W3111482049 cites W1966616903 @default.
- W3111482049 cites W1968682299 @default.
- W3111482049 cites W1969787762 @default.
- W3111482049 cites W1975945730 @default.
- W3111482049 cites W1979166300 @default.
- W3111482049 cites W1980478162 @default.
- W3111482049 cites W1989035987 @default.
- W3111482049 cites W1992523293 @default.
- W3111482049 cites W1996183144 @default.
- W3111482049 cites W1999077168 @default.
- W3111482049 cites W1999483871 @default.
- W3111482049 cites W2002193732 @default.
- W3111482049 cites W2003560901 @default.
- W3111482049 cites W2010786308 @default.
- W3111482049 cites W2017551575 @default.
- W3111482049 cites W2026727321 @default.
- W3111482049 cites W2034981907 @default.
- W3111482049 cites W2036656105 @default.
- W3111482049 cites W2038179254 @default.
- W3111482049 cites W2039795021 @default.
- W3111482049 cites W2040937367 @default.
- W3111482049 cites W2043272929 @default.
- W3111482049 cites W2049936368 @default.
- W3111482049 cites W2057569679 @default.
- W3111482049 cites W2058625457 @default.
- W3111482049 cites W2060620553 @default.
- W3111482049 cites W2061830113 @default.
- W3111482049 cites W2066623659 @default.
- W3111482049 cites W2067693929 @default.
- W3111482049 cites W2068689195 @default.
- W3111482049 cites W2069856609 @default.
- W3111482049 cites W2070086938 @default.
- W3111482049 cites W2071209026 @default.
- W3111482049 cites W2071251525 @default.
- W3111482049 cites W2071914336 @default.
- W3111482049 cites W2072088396 @default.
- W3111482049 cites W2079094640 @default.
- W3111482049 cites W2081606553 @default.
- W3111482049 cites W2082355480 @default.
- W3111482049 cites W2092841704 @default.
- W3111482049 cites W2092997319 @default.
- W3111482049 cites W2095281125 @default.
- W3111482049 cites W2096025413 @default.
- W3111482049 cites W2099760694 @default.
- W3111482049 cites W2109982506 @default.
- W3111482049 cites W2114580309 @default.
- W3111482049 cites W2116349394 @default.
- W3111482049 cites W2131317050 @default.
- W3111482049 cites W2140260269 @default.
- W3111482049 cites W2145000518 @default.
- W3111482049 cites W2146145365 @default.
- W3111482049 cites W2157957881 @default.
- W3111482049 cites W2162546635 @default.
- W3111482049 cites W2167943223 @default.
- W3111482049 cites W2168792225 @default.
- W3111482049 cites W2172850461 @default.
- W3111482049 cites W2177434887 @default.
- W3111482049 cites W2280975571 @default.
- W3111482049 cites W2327828849 @default.
- W3111482049 cites W2342950527 @default.
- W3111482049 cites W2368005800 @default.
- W3111482049 cites W2415105986 @default.
- W3111482049 cites W2470057990 @default.
- W3111482049 cites W2601080948 @default.
- W3111482049 cites W2731233839 @default.
- W3111482049 cites W2770725028 @default.
- W3111482049 cites W2773094630 @default.
- W3111482049 cites W2779703523 @default.
- W3111482049 cites W2808350721 @default.
- W3111482049 cites W2890453231 @default.
- W3111482049 cites W2936469149 @default.
- W3111482049 cites W2944945365 @default.
- W3111482049 cites W2950168265 @default.
- W3111482049 cites W2981908669 @default.
- W3111482049 cites W3012099880 @default.
- W3111482049 cites W3017840536 @default.
- W3111482049 cites W3037331781 @default.
- W3111482049 cites W3039810691 @default.
- W3111482049 cites W3041334230 @default.
- W3111482049 cites W3110343827 @default.
- W3111482049 cites W4239340399 @default.
- W3111482049 doi "https://doi.org/10.1038/s41598-020-79327-6" @default.
- W3111482049 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7746737" @default.
- W3111482049 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33335248" @default.
- W3111482049 hasPublicationYear "2020" @default.
- W3111482049 type Work @default.
- W3111482049 sameAs 3111482049 @default.