FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3111645510> ?p ?o ?g. }

• W3111645510 endingPage "286" @default.
• W3111645510 startingPage "286" @default.
• W3111645510 abstract "<ns4:p><ns4:bold>Background:</ns4:bold> <ns4:italic>Staphylococcus aureus</ns4:italic> causes an array of diseases in both humans and livestock. Pathogenesis is mediated by a plethora of proteins secreted by <ns4:italic>S. aureus</ns4:italic>, many of which remain incompletely characterised. For example, <ns4:italic>S. aureus</ns4:italic> abundantly secretes two isoforms of the enzyme lipase into the extracellular milieu, where they scavenge upon polymeric triglycerides. It has previously been suggested that lipases may interfere with the function of innate immune cells, such as macrophages and neutrophils, but the impact of lipases on phagocytic killing mechanisms remains unknown.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> We employed the epidemic <ns4:italic>S. aureus</ns4:italic> clone USA300 strain LAC and its lipase deficient isogenic mutant, along with recombinant lipase proteins, in <ns4:italic>in vitro</ns4:italic> experimental infection assays. To determine if lipases can inhibit innate immune killing mechanisms, the bactericidal activity of whole blood, human neutrophils, and macrophages was analysed. In addition, gentamycin protection assays were carried out to examine the influence of lipases on <ns4:italic>S. aureus</ns4:italic> innate immune cell escape.</ns4:p><ns4:p> <ns4:bold>Results:</ns4:bold> There were no differences in the survival of <ns4:italic>S. aureus</ns4:italic> USA300 LAC wild type and its lipase-deficient isogenic mutant after incubation with human whole blood or neutrophils. Furthermore, there was no detectable lipase-dependent effect on phagocytosis, intracellular survival, or escape from both human primary and immortalised cell line macrophages, even upon supplementation with exogenous recombinant lipases.</ns4:p><ns4:p> <ns4:bold>Conclusions: </ns4:bold><ns4:italic>S. aureus</ns4:italic> lipases do not inhibit bacterial killing mechanisms of human macrophages, neutrophils, or whole blood. These findings broaden our understanding of the interaction of <ns4:italic>S. aureus</ns4:italic> with the innate immune system.</ns4:p>" @default.
• W3111645510 created "2020-12-21" @default.
• W3111645510 creator A5016368703 @default.
• W3111645510 creator A5026276536 @default.
• W3111645510 creator A5030623005 @default.
• W3111645510 creator A5068159168 @default.
• W3111645510 creator A5079458165 @default.
• W3111645510 date "2021-06-25" @default.
• W3111645510 modified "2023-10-07" @default.
• W3111645510 title "Staphylococcus aureus secreted lipases do not inhibit innate immune killing mechanisms" @default.
• W3111645510 cites W1896283776 @default.
• W3111645510 cites W1907075739 @default.
• W3111645510 cites W1964038763 @default.
• W3111645510 cites W1972027632 @default.
• W3111645510 cites W1976160873 @default.
• W3111645510 cites W1978563472 @default.
• W3111645510 cites W1981793562 @default.
• W3111645510 cites W1985309974 @default.
• W3111645510 cites W1986442317 @default.
• W3111645510 cites W2017821807 @default.
• W3111645510 cites W2021866013 @default.
• W3111645510 cites W2042984912 @default.
• W3111645510 cites W2087038557 @default.
• W3111645510 cites W2095357894 @default.
• W3111645510 cites W2110763275 @default.
• W3111645510 cites W2118342981 @default.
• W3111645510 cites W2119727748 @default.
• W3111645510 cites W2126488946 @default.
• W3111645510 cites W2147425799 @default.
• W3111645510 cites W2148265829 @default.
• W3111645510 cites W2159153392 @default.
• W3111645510 cites W2166227322 @default.
• W3111645510 cites W2176306829 @default.
• W3111645510 cites W2179197851 @default.
• W3111645510 cites W2286083932 @default.
• W3111645510 cites W2530424978 @default.
• W3111645510 cites W2741409856 @default.
• W3111645510 cites W2770277250 @default.
• W3111645510 cites W2790538253 @default.
• W3111645510 cites W2891959954 @default.
• W3111645510 cites W2913035670 @default.
• W3111645510 cites W2966212283 @default.
• W3111645510 cites W3038480290 @default.
• W3111645510 doi "https://doi.org/10.12688/wellcomeopenres.16194.2" @default.
• W3111645510 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7871421" @default.
• W3111645510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33623827" @default.
• W3111645510 hasPublicationYear "2021" @default.
• W3111645510 type Work @default.
• W3111645510 sameAs 3111645510 @default.
• W3111645510 citedByCount "2" @default.
• W3111645510 countsByYear W31116455102023 @default.
• W3111645510 crossrefType "journal-article" @default.
• W3111645510 hasAuthorship W3111645510A5016368703 @default.
• W3111645510 hasAuthorship W3111645510A5026276536 @default.
• W3111645510 hasAuthorship W3111645510A5030623005 @default.
• W3111645510 hasAuthorship W3111645510A5068159168 @default.
• W3111645510 hasAuthorship W3111645510A5079458165 @default.
• W3111645510 hasBestOaLocation W31116455101 @default.
• W3111645510 hasConcept C136449434 @default.
• W3111645510 hasConcept C159047783 @default.
• W3111645510 hasConcept C203014093 @default.
• W3111645510 hasConcept C86803240 @default.
• W3111645510 hasConcept C8891405 @default.
• W3111645510 hasConceptScore W3111645510C136449434 @default.
• W3111645510 hasConceptScore W3111645510C159047783 @default.
• W3111645510 hasConceptScore W3111645510C203014093 @default.
• W3111645510 hasConceptScore W3111645510C86803240 @default.
• W3111645510 hasConceptScore W3111645510C8891405 @default.
• W3111645510 hasFunder F4320311904 @default.
• W3111645510 hasFunder F4320334629 @default.
• W3111645510 hasLocation W31116455101 @default.
• W3111645510 hasLocation W31116455102 @default.
• W3111645510 hasLocation W31116455103 @default.
• W3111645510 hasOpenAccess W3111645510 @default.
• W3111645510 hasPrimaryLocation W31116455101 @default.
• W3111645510 hasRelatedWork W1524155111 @default.
• W3111645510 hasRelatedWork W1556307916 @default.
• W3111645510 hasRelatedWork W1980618878 @default.
• W3111645510 hasRelatedWork W2008306603 @default.
• W3111645510 hasRelatedWork W2048363111 @default.
• W3111645510 hasRelatedWork W2084905130 @default.
• W3111645510 hasRelatedWork W2103515427 @default.
• W3111645510 hasRelatedWork W2141330728 @default.
• W3111645510 hasRelatedWork W2946615038 @default.
• W3111645510 hasRelatedWork W4315797293 @default.
• W3111645510 hasVolume "5" @default.
• W3111645510 isParatext "false" @default.
• W3111645510 isRetracted "false" @default.
• W3111645510 magId "3111645510" @default.
• W3111645510 workType "article" @default.