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• W3111712100 abstract "Abstract The personal glucose meter (PGM) is one of the most successful point-of-care (POC) testing devices. It is simple, robust and inexpensive, but cannot be easily adapted to analytes other than glucose. We report a novel chemical conjugation-based assay strategy, using rational design of chemically-derivatized glucose-encapsulating liposomes, to repurpose a PGM, taking an important mycotoxin patulin as the model analyte. Sulfhydryl (–SH) was proposed for the first time as a specific functional group for efficient recognition of patulin. Multifunctional sulfhydryl-terminated glucose-encapsulating liposomes (G-LIP-SH) were synthesized in a simple, single step, which efficiently captured patulin by covalent bonding, and interacted strongly with NH2–Au@Fe3O4 nanoparticles. Magnetic removal of nanoparticles efficiently and selectively separated patulin-derivatized from un-derivatized G-LIP-SH, permitting the latter to be lysed and the released glucose measured by PGM. The PGM signal was inversely proportional to the patulin concentration, over the range of 0.1–50 ng mL−1 (R2 = 0.995) with a detection limit of 0.05 ng mL−1 (S/N = 3). This approach overcame interference from endogenous glucose, other mycotoxins and metal ions, allowing the analysis of a wide range of sample matrices and showed high specificity, acceptable reproducibility, good accuracy and optimal applicability. Other derivatization chemistries will enable this approach to be adapted to analytes with a wide range of chemical structures, to facilitate development of rapid, portable, user-friendly and cost-effective assays applicable to diverse analytes and sample matrices." @default.
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• W3111712100 date "2021-03-01" @default.
• W3111712100 modified "2023-09-23" @default.
• W3111712100 title "A flexible assay strategy for non-glucose targets based on sulfhydryl-terminated liposomes combined with personal glucometer" @default.
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• W3111712100 doi "https://doi.org/10.1016/j.bios.2020.112884" @default.
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