Matches in SemOpenAlex for { <https://semopenalex.org/work/W3112351068> ?p ?o ?g. }
- W3112351068 endingPage "9604" @default.
- W3112351068 startingPage "9604" @default.
- W3112351068 abstract "The discovery of clustered, regularly interspaced short palindromic repeats (CRISPR) and their cooperation with CRISPR-associated (Cas) genes is one of the greatest advances of the century and has marked their application as a powerful genome engineering tool. The CRISPR–Cas system was discovered as a part of the adaptive immune system in bacteria and archaea to defend from plasmids and phages. CRISPR has been found to be an advanced alternative to zinc-finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN) for gene editing and regulation, as the CRISPR–Cas9 protein remains the same for various gene targets and just a short guide RNA sequence needs to be altered to redirect the site-specific cleavage. Due to its high efficiency and precision, the Cas9 protein derived from the type II CRISPR system has been found to have applications in many fields of science. Although CRISPR–Cas9 allows easy genome editing and has a number of benefits, we should not ignore the important ethical and biosafety issues. Moreover, any tool that has great potential and offers significant capabilities carries a level of risk of being used for non-legal purposes. In this review, we present a brief history and mechanism of the CRISPR–Cas9 system. We also describe on the applications of this technology in gene regulation and genome editing; the treatment of cancer and other diseases; and limitations and concerns of the use of CRISPR–Cas9." @default.
- W3112351068 created "2020-12-21" @default.
- W3112351068 creator A5027535776 @default.
- W3112351068 creator A5033678080 @default.
- W3112351068 creator A5038486055 @default.
- W3112351068 creator A5060187731 @default.
- W3112351068 creator A5068754660 @default.
- W3112351068 creator A5071872804 @default.
- W3112351068 date "2020-12-16" @default.
- W3112351068 modified "2023-10-10" @default.
- W3112351068 title "Various Aspects of a Gene Editing System—CRISPR–Cas9" @default.
- W3112351068 cites W1616809472 @default.
- W3112351068 cites W1852971431 @default.
- W3112351068 cites W1973260880 @default.
- W3112351068 cites W1976391205 @default.
- W3112351068 cites W1978094899 @default.
- W3112351068 cites W1982873976 @default.
- W3112351068 cites W1990487336 @default.
- W3112351068 cites W1993013666 @default.
- W3112351068 cites W1996788258 @default.
- W3112351068 cites W1997060995 @default.
- W3112351068 cites W1998630795 @default.
- W3112351068 cites W2001497035 @default.
- W3112351068 cites W2001962483 @default.
- W3112351068 cites W2003171404 @default.
- W3112351068 cites W2016033442 @default.
- W3112351068 cites W2019670881 @default.
- W3112351068 cites W2022216399 @default.
- W3112351068 cites W2026520402 @default.
- W3112351068 cites W2045435533 @default.
- W3112351068 cites W2058873531 @default.
- W3112351068 cites W2060297895 @default.
- W3112351068 cites W2064815984 @default.
- W3112351068 cites W2067119305 @default.
- W3112351068 cites W2070614858 @default.
- W3112351068 cites W2077190448 @default.
- W3112351068 cites W2083582836 @default.
- W3112351068 cites W2084747588 @default.
- W3112351068 cites W2086706302 @default.
- W3112351068 cites W2089929813 @default.
- W3112351068 cites W2091278469 @default.
- W3112351068 cites W2093814864 @default.
- W3112351068 cites W2097379120 @default.
- W3112351068 cites W2102552859 @default.
- W3112351068 cites W2115268444 @default.
- W3112351068 cites W2116565356 @default.
- W3112351068 cites W2118746294 @default.
- W3112351068 cites W2118796952 @default.
- W3112351068 cites W2119771879 @default.
- W3112351068 cites W2120932131 @default.
- W3112351068 cites W2122941722 @default.
- W3112351068 cites W2123965484 @default.
- W3112351068 cites W2132401721 @default.
- W3112351068 cites W2137721714 @default.
- W3112351068 cites W2138993899 @default.
- W3112351068 cites W2140754284 @default.
- W3112351068 cites W2141825721 @default.
- W3112351068 cites W2144134062 @default.
- W3112351068 cites W2150575159 @default.
- W3112351068 cites W2153344788 @default.
- W3112351068 cites W2153355254 @default.
- W3112351068 cites W2155660947 @default.
- W3112351068 cites W2158637234 @default.
- W3112351068 cites W2160051211 @default.
- W3112351068 cites W2163241233 @default.
- W3112351068 cites W2163248265 @default.
- W3112351068 cites W2166139447 @default.
- W3112351068 cites W2169244146 @default.
- W3112351068 cites W2208696160 @default.
- W3112351068 cites W2239511084 @default.
- W3112351068 cites W2260024370 @default.
- W3112351068 cites W2260480416 @default.
- W3112351068 cites W2291052616 @default.
- W3112351068 cites W2292390328 @default.
- W3112351068 cites W2302341612 @default.
- W3112351068 cites W2304692062 @default.
- W3112351068 cites W2320086560 @default.
- W3112351068 cites W2325985500 @default.
- W3112351068 cites W2336828812 @default.
- W3112351068 cites W2340099071 @default.
- W3112351068 cites W2484900834 @default.
- W3112351068 cites W2519958822 @default.
- W3112351068 cites W2524784061 @default.
- W3112351068 cites W2527008945 @default.
- W3112351068 cites W2531490221 @default.
- W3112351068 cites W2580171608 @default.
- W3112351068 cites W2585802341 @default.
- W3112351068 cites W2588152092 @default.
- W3112351068 cites W2593691947 @default.
- W3112351068 cites W2593766584 @default.
- W3112351068 cites W2618393503 @default.
- W3112351068 cites W2623277795 @default.
- W3112351068 cites W2626278637 @default.
- W3112351068 cites W2729411160 @default.
- W3112351068 cites W2751474071 @default.
- W3112351068 cites W2766599608 @default.
- W3112351068 cites W2767657905 @default.
- W3112351068 cites W2771278775 @default.