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- W3112453485 abstract "This study aimed to investigate the molecular characterization, antioxidant activity in vitro, cytotoxicity study of an exopolysaccharide isolated from Citrobacter freundii. Firstly, the culture conditions were standardized by the Design of experiments (DoE) based approach, and the final yield of thecrude exopolysaccharide was optimized at 2568 ± 169 mg L−1. One large fraction of exopolysaccharide was obtained from the culture filtrate by size exclusion chromatography and molecular characteristics were studied. A new mannose rich exopolysaccharide (Fraction-I) with average molecular weight ~ 1.34 × 105 Da was isolated. The sugar analysis showed the presence of mannose and glucose in a molar ratio of nearly 7:2 respectively. The structure of the repeating unit in the exopolysaccharide was determined through chemical and 1D/2D- NMR experiments as:Download : Download high-res image (71KB)Download : Download full-size image Finally, the antioxidant activity, and the cytotoxicity of the exopolysaccharide were investigated and the relationship with molecular properties was discussed as well." @default.
- W3112453485 created "2020-12-21" @default.
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- W3112453485 date "2021-01-01" @default.
- W3112453485 modified "2023-09-24" @default.
- W3112453485 title "Structure and biological properties of exopolysaccharide isolated from Citrobacter freundii" @default.
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- W3112453485 doi "https://doi.org/10.1016/j.ijbiomac.2020.12.063" @default.
- W3112453485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33316341" @default.
- W3112453485 hasPublicationYear "2021" @default.
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