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- W3112482011 abstract "Flow cytometry (FCM) is widely used to characterize cellular phenotypes at the single-cell level. In combination with intracellular staining, this is a well-known method for detecting cytosolic or nuclear proteins that require cell fixation and permeabilization of the cell membrane for detection [ [1] Jung T. Schauer U. Heusser C. Neumann C. Rieger C. Detection of intracellular cytokines by flow cytometry. J. Immunol. Methods. 1993; 159: 197-207 Crossref PubMed Scopus (887) Google Scholar , [2] Adan A. Alizada G. Kiraz Y Y. Baran Y. Nalbant A. Flow cytometry: basic principles and applications. Crit. Rev. Biotechnol. 2017; 37: 163-176 Crossref PubMed Scopus (282) Google Scholar ]. Although a general protocol for intracellular staining is applicable to most cytosolic proteins, to obtain reliable results in Foxp3 detection, a special staining kit with specific instructions is recommended for the staining of Foxp3 [ [3] Sun Y.L. Lin G.G. Zhang K. Wang L.N. Li J.M. Application and effects of mouse Foxp3 antibody and fixation/permeabilization buffer on the detection of CD4+ regulatory T cells in various mammal species. Genet. Mol. Res. 2013; 12: 6535-6545 Crossref PubMed Scopus (9) Google Scholar ], a master transcription factor of regulatory T cells (Tregs) [ [4] Hori S. Nomura T. Sakaguchi S. Control of regulatory T cell development by the transcription factor Foxp3. Science. 2003; 299: 1057-1061 Crossref PubMed Scopus (37) Google Scholar ]." @default.
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- W3112482011 date "2021-02-01" @default.
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- W3112482011 title "Simultaneous detection of mouse Foxp3 and cytosolic fluorescent protein by a modified intracellular staining protocol" @default.
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- W3112482011 doi "https://doi.org/10.1016/j.jdermsci.2020.10.017" @default.
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