Matches in SemOpenAlex for { <https://semopenalex.org/work/W3112553485> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W3112553485 abstract "The recently introduced microphysiological systems (MPS) cultivating human organoids are expected to perform better than animals in the preclinical tests phase of drug developing process because they are genetically human and recapitulate the interplay among tissues. In this study, the human intestinal barrier (emulated by a co-culture of Caco-2 and HT-29 cells) and the liver equivalent (emulated by spheroids made of differentiated HepaRG cells and human hepatic stellate cells) were integrated into a two-organ chip (2-OC) microfluidic device to assess some acetaminophen (APAP) pharmacokinetic (PK) and toxicological properties. The MPS had three assemblies: Intestine only 2-OC, Liver only 2-OC, and Intestine/Liver 2-OC with the same media perfusing both organoids. For PK assessments, we dosed the APAP in the media at preset timepoints after administering it either over the intestinal barrier (emulating the oral route) or in the media (emulating the intravenous route), at 12 µM and 2 µM respectively. The media samples were analyzed by reversed-phase high-pressure liquid chromatography (HPLC). Organoids were analyzed for gene expression, for TEER values, for protein expression and activity, and then collected, fixed, and submitted to a set of morphological evaluations. The MTT technique performed well in assessing the organoid viability, but the high content analyses (HCA) were able to detect very early toxic events in response to APAP treatment. We verified that the media flow does not significantly affect the APAP absorption whereas it significantly improves the liver equivalent functionality. The APAP human intestinal absorption and hepatic metabolism could be emulated in the MPS. The association between MPS data and in silico modeling has great potential to improve the predictability of the in vitro methods and provide better accuracy than animal models in pharmacokinetic and toxicological studies." @default.
- W3112553485 created "2020-12-21" @default.
- W3112553485 creator A5010440123 @default.
- W3112553485 creator A5016289007 @default.
- W3112553485 creator A5028401971 @default.
- W3112553485 creator A5054067127 @default.
- W3112553485 creator A5054602755 @default.
- W3112553485 creator A5058433973 @default.
- W3112553485 creator A5074587582 @default.
- W3112553485 creator A5083808565 @default.
- W3112553485 creator A5084744093 @default.
- W3112553485 creator A5089465087 @default.
- W3112553485 date "2020-12-03" @default.
- W3112553485 modified "2023-10-14" @default.
- W3112553485 title "An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment" @default.
- W3112553485 doi "https://doi.org/10.3791/60184" @default.
- W3112553485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33346185" @default.
- W3112553485 hasPublicationYear "2020" @default.
- W3112553485 type Work @default.
- W3112553485 sameAs 3112553485 @default.
- W3112553485 citedByCount "2" @default.
- W3112553485 countsByYear W31125534852021 @default.
- W3112553485 crossrefType "journal-article" @default.
- W3112553485 hasAuthorship W3112553485A5010440123 @default.
- W3112553485 hasAuthorship W3112553485A5016289007 @default.
- W3112553485 hasAuthorship W3112553485A5028401971 @default.
- W3112553485 hasAuthorship W3112553485A5054067127 @default.
- W3112553485 hasAuthorship W3112553485A5054602755 @default.
- W3112553485 hasAuthorship W3112553485A5058433973 @default.
- W3112553485 hasAuthorship W3112553485A5074587582 @default.
- W3112553485 hasAuthorship W3112553485A5083808565 @default.
- W3112553485 hasAuthorship W3112553485A5084744093 @default.
- W3112553485 hasAuthorship W3112553485A5089465087 @default.
- W3112553485 hasConcept C112705442 @default.
- W3112553485 hasConcept C121332964 @default.
- W3112553485 hasConcept C125287762 @default.
- W3112553485 hasConcept C142724271 @default.
- W3112553485 hasConcept C176891718 @default.
- W3112553485 hasConcept C179998833 @default.
- W3112553485 hasConcept C185592680 @default.
- W3112553485 hasConcept C201253974 @default.
- W3112553485 hasConcept C24890656 @default.
- W3112553485 hasConcept C2777882243 @default.
- W3112553485 hasConcept C2778722691 @default.
- W3112553485 hasConcept C2780035454 @default.
- W3112553485 hasConcept C31695470 @default.
- W3112553485 hasConcept C43617362 @default.
- W3112553485 hasConcept C55493867 @default.
- W3112553485 hasConcept C71924100 @default.
- W3112553485 hasConcept C86803240 @default.
- W3112553485 hasConcept C89311334 @default.
- W3112553485 hasConcept C95444343 @default.
- W3112553485 hasConcept C98274493 @default.
- W3112553485 hasConceptScore W3112553485C112705442 @default.
- W3112553485 hasConceptScore W3112553485C121332964 @default.
- W3112553485 hasConceptScore W3112553485C125287762 @default.
- W3112553485 hasConceptScore W3112553485C142724271 @default.
- W3112553485 hasConceptScore W3112553485C176891718 @default.
- W3112553485 hasConceptScore W3112553485C179998833 @default.
- W3112553485 hasConceptScore W3112553485C185592680 @default.
- W3112553485 hasConceptScore W3112553485C201253974 @default.
- W3112553485 hasConceptScore W3112553485C24890656 @default.
- W3112553485 hasConceptScore W3112553485C2777882243 @default.
- W3112553485 hasConceptScore W3112553485C2778722691 @default.
- W3112553485 hasConceptScore W3112553485C2780035454 @default.
- W3112553485 hasConceptScore W3112553485C31695470 @default.
- W3112553485 hasConceptScore W3112553485C43617362 @default.
- W3112553485 hasConceptScore W3112553485C55493867 @default.
- W3112553485 hasConceptScore W3112553485C71924100 @default.
- W3112553485 hasConceptScore W3112553485C86803240 @default.
- W3112553485 hasConceptScore W3112553485C89311334 @default.
- W3112553485 hasConceptScore W3112553485C95444343 @default.
- W3112553485 hasConceptScore W3112553485C98274493 @default.
- W3112553485 hasIssue "166" @default.
- W3112553485 hasLocation W31125534851 @default.
- W3112553485 hasOpenAccess W3112553485 @default.
- W3112553485 hasPrimaryLocation W31125534851 @default.
- W3112553485 hasRelatedWork W1969876219 @default.
- W3112553485 hasRelatedWork W2037803563 @default.
- W3112553485 hasRelatedWork W2124775757 @default.
- W3112553485 hasRelatedWork W2131557988 @default.
- W3112553485 hasRelatedWork W2889241014 @default.
- W3112553485 hasRelatedWork W3024440267 @default.
- W3112553485 hasRelatedWork W4205993769 @default.
- W3112553485 hasRelatedWork W4285297479 @default.
- W3112553485 hasRelatedWork W4320061796 @default.
- W3112553485 hasRelatedWork W4365135207 @default.
- W3112553485 isParatext "false" @default.
- W3112553485 isRetracted "false" @default.
- W3112553485 magId "3112553485" @default.
- W3112553485 workType "article" @default.