FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3112643739> ?p ?o ?g. }

• W3112643739 endingPage "112449" @default.
• W3112643739 startingPage "112449" @default.
• W3112643739 abstract "Delayed endothelial healing after drug eluting stent (DES) implantation is a critical clinical problem in treatment of coronary artery diseases. Exosomes exhibit proangiogenic potential in a variety of ischemic diseases. However, the association of exosomes with endothelial regeneration after DES implantation has been rarely reported. In this study, we aimed to investigate the therapeutic effects of mesenchymal stem cell (MSC)-derived exosomes on endothelial cells treated with rapamycin and explore the potential mechanisms of MSC-derived exosomes in promoting endothelial regeneration. Exosomes were isolated from MSCs by ultracentrifugation and identified by transmission electron microscopy, nanoparticle tracking analysis, and Western blot assay. The in vitro effects of MSC-derived exosomes on the proliferation and migration of endothelial cells treated with rapamycin were evaluated by integrated experiment, cell counting kit-8, scratch, tube formation, and transwell assays. And the apoptosis of rapamycin-induced endothelial cells loaded with MSC-derived exosomes was detected using TUNEL and Annexin-V FITC and PI double-staining assays. The microRNA (miRNA) cargo of MSC-derived exosomes was identified by high-throughput RNA sequencing. Pro-angiogenic miRNAs and key pathways were further characterized. Our results indicated that MSC-derived exosomes could be ingested into umbilical vein endothelial cells (HUVECs) and significantly enhanced cell proliferation rate, migratory and tube-forming capabilities in vitro. MSC-derived exosomes also inhibited the apoptosis of HUVECs induced by rapamycin. A distinct class of exosomal miRNAs was further identified, including six miRNAs tightly related to neovasculogenesis. Silencing the expression of exosomal miRNA-21–5p and let-7c-5p attenuated the pro-proliferative and pro-migratory capacity of MSC-derived exosomes. Moreover, functional enrichment analysis indicated that metabolic pathways might contribute to reendothelialization. This study highlights a proregenerative effect of MSC-derived exosomes in vitro, which may be partly explained by the delivery of pro-angiogenic miRNAs to endothelial cells." @default.
• W3112643739 created "2020-12-21" @default.
• W3112643739 creator A5011978576 @default.
• W3112643739 creator A5046078938 @default.
• W3112643739 creator A5054594638 @default.
• W3112643739 creator A5060757292 @default.
• W3112643739 creator A5070348247 @default.
• W3112643739 creator A5075193406 @default.
• W3112643739 creator A5075983724 @default.
• W3112643739 creator A5077951238 @default.
• W3112643739 creator A5088092323 @default.
• W3112643739 date "2021-02-01" @default.
• W3112643739 modified "2023-10-16" @default.
• W3112643739 title "Exosomes secreted from mesenchymal stem cells mediate the regeneration of endothelial cells treated with rapamycin by delivering pro-angiogenic microRNAs" @default.
• W3112643739 cites W1493025853 @default.
• W3112643739 cites W1863304666 @default.
• W3112643739 cites W1902734556 @default.
• W3112643739 cites W1977692564 @default.
• W3112643739 cites W1998823652 @default.
• W3112643739 cites W2001049670 @default.
• W3112643739 cites W2001089762 @default.
• W3112643739 cites W2009645185 @default.
• W3112643739 cites W2012549032 @default.
• W3112643739 cites W2025371271 @default.
• W3112643739 cites W2070257574 @default.
• W3112643739 cites W2107206514 @default.
• W3112643739 cites W2114222046 @default.
• W3112643739 cites W2141165366 @default.
• W3112643739 cites W2180107626 @default.
• W3112643739 cites W2419151490 @default.
• W3112643739 cites W2564532459 @default.
• W3112643739 cites W2590694304 @default.
• W3112643739 cites W2603453711 @default.
• W3112643739 cites W2605245050 @default.
• W3112643739 cites W2736433988 @default.
• W3112643739 cites W2766582147 @default.
• W3112643739 cites W2770714340 @default.
• W3112643739 cites W2771058495 @default.
• W3112643739 cites W2782469869 @default.
• W3112643739 cites W2795067181 @default.
• W3112643739 cites W2906254229 @default.
• W3112643739 cites W2911533047 @default.
• W3112643739 cites W2912858542 @default.
• W3112643739 cites W2914854889 @default.
• W3112643739 cites W2919741116 @default.
• W3112643739 cites W2939945220 @default.
• W3112643739 cites W2941685970 @default.
• W3112643739 cites W2943637321 @default.
• W3112643739 cites W2956316731 @default.
• W3112643739 cites W2991540267 @default.
• W3112643739 cites W3008788563 @default.
• W3112643739 cites W3013531546 @default.
• W3112643739 cites W3022186628 @default.
• W3112643739 doi "https://doi.org/10.1016/j.yexcr.2020.112449" @default.
• W3112643739 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33347856" @default.
• W3112643739 hasPublicationYear "2021" @default.
• W3112643739 type Work @default.
• W3112643739 sameAs 3112643739 @default.
• W3112643739 citedByCount "9" @default.
• W3112643739 countsByYear W31126437392022 @default.
• W3112643739 countsByYear W31126437392023 @default.
• W3112643739 crossrefType "journal-article" @default.
• W3112643739 hasAuthorship W3112643739A5011978576 @default.
• W3112643739 hasAuthorship W3112643739A5046078938 @default.
• W3112643739 hasAuthorship W3112643739A5054594638 @default.
• W3112643739 hasAuthorship W3112643739A5060757292 @default.
• W3112643739 hasAuthorship W3112643739A5070348247 @default.
• W3112643739 hasAuthorship W3112643739A5075193406 @default.
• W3112643739 hasAuthorship W3112643739A5075983724 @default.
• W3112643739 hasAuthorship W3112643739A5077951238 @default.
• W3112643739 hasAuthorship W3112643739A5088092323 @default.
• W3112643739 hasConcept C104317684 @default.
• W3112643739 hasConcept C123012128 @default.
• W3112643739 hasConcept C145059251 @default.
• W3112643739 hasConcept C198826908 @default.
• W3112643739 hasConcept C202751555 @default.
• W3112643739 hasConcept C203014093 @default.
• W3112643739 hasConcept C20518536 @default.
• W3112643739 hasConcept C2780394083 @default.
• W3112643739 hasConcept C2781261824 @default.
• W3112643739 hasConcept C502942594 @default.
• W3112643739 hasConcept C553184892 @default.
• W3112643739 hasConcept C55493867 @default.
• W3112643739 hasConcept C86803240 @default.
• W3112643739 hasConcept C88634738 @default.
• W3112643739 hasConcept C95444343 @default.
• W3112643739 hasConceptScore W3112643739C104317684 @default.
• W3112643739 hasConceptScore W3112643739C123012128 @default.
• W3112643739 hasConceptScore W3112643739C145059251 @default.
• W3112643739 hasConceptScore W3112643739C198826908 @default.
• W3112643739 hasConceptScore W3112643739C202751555 @default.
• W3112643739 hasConceptScore W3112643739C203014093 @default.
• W3112643739 hasConceptScore W3112643739C20518536 @default.
• W3112643739 hasConceptScore W3112643739C2780394083 @default.
• W3112643739 hasConceptScore W3112643739C2781261824 @default.
• W3112643739 hasConceptScore W3112643739C502942594 @default.
• W3112643739 hasConceptScore W3112643739C553184892 @default.
• W3112643739 hasConceptScore W3112643739C55493867 @default.

• next