FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3112740601> ?p ?o ?g. }

• W3112740601 endingPage "9494" @default.
• W3112740601 startingPage "9494" @default.
• W3112740601 abstract "Pseudomonas aeruginosa is an opportunistic pathogen that causes biofilm-associated infections. P. aeruginosa can survive in a dormant state with reduced metabolic activity in nutrient-limited environments, including the interiors of biofilms. When entering dormancy, the bacteria undergo metabolic remodeling, which includes reduced translation and degradation of cellular proteins. However, a supply of essential macromolecules, such as ribosomes, are protected from degradation during dormancy. The small ribosome-binding proteins, hibernation promoting factor (HPF) and ribosome modulation factor (RMF), inhibit translation by inducing formation of inactive 70S and 100S ribosome monomers and dimers. The inactivated ribosomes are protected from the initial steps in ribosome degradation, including endonuclease cleavage of the ribosomal RNA (rRNA). Here, we characterized the role of HPF in ribosomal protein (rProtein) stability and degradation during P. aeruginosa nutrient limitation. We determined the effect of the physiological status of P. aeruginosa prior to starvation on its ability to recover from starvation, and on its rRNA and rProtein stability during cell starvation. The results show that the wild-type strain and a stringent response mutant (∆relA∆spoT strain) maintain high cellular abundances of the rProteins L5 and S13 over the course of eight days of starvation. In contrast, the abundances of L5 and S13 reduce in the ∆hpf mutant cells. The loss of rProteins in the ∆hpf strain is dependent on the physiology of the cells prior to starvation. The greatest rProtein loss occurs when cells are first cultured to stationary phase prior to starvation, with less rProtein loss in the ∆hpf cells that are first cultured to exponential phase or in balanced minimal medium. Regardless of the pre-growth conditions, P. aeruginosa recovery from starvation and the integrity of its rRNA are impaired in the absence of HPF. The results indicate that protein remodeling during P. aeruginosa starvation includes the degradation of rProteins, and that HPF is essential to prevent rProtein loss in starved P. aeruginosa. The results also indicate that HPF is produced throughout cell growth, and that regardless of the cellular physiological status, HPF is required to protect against ribosome loss when the cells subsequently enter starvation phase." @default.
• W3112740601 created "2020-12-21" @default.
• W3112740601 creator A5002137230 @default.
• W3112740601 creator A5074065701 @default.
• W3112740601 creator A5074220233 @default.
• W3112740601 date "2020-12-14" @default.
• W3112740601 modified "2023-09-25" @default.
• W3112740601 title "Role of Hibernation Promoting Factor in Ribosomal Protein Stability during Pseudomonas aeruginosa Dormancy" @default.
• W3112740601 cites W1516043067 @default.
• W3112740601 cites W1519877831 @default.
• W3112740601 cites W1967239401 @default.
• W3112740601 cites W1972374219 @default.
• W3112740601 cites W1974544574 @default.
• W3112740601 cites W1975141449 @default.
• W3112740601 cites W1981307422 @default.
• W3112740601 cites W1986842298 @default.
• W3112740601 cites W1988067154 @default.
• W3112740601 cites W1996064131 @default.
• W3112740601 cites W1998125347 @default.
• W3112740601 cites W2014695795 @default.
• W3112740601 cites W2019347117 @default.
• W3112740601 cites W2024837019 @default.
• W3112740601 cites W2045915531 @default.
• W3112740601 cites W2052652877 @default.
• W3112740601 cites W2057015838 @default.
• W3112740601 cites W2060367587 @default.
• W3112740601 cites W2069549031 @default.
• W3112740601 cites W2073824449 @default.
• W3112740601 cites W2074058520 @default.
• W3112740601 cites W2085630244 @default.
• W3112740601 cites W2096085685 @default.
• W3112740601 cites W2097225375 @default.
• W3112740601 cites W2100619015 @default.
• W3112740601 cites W2107734506 @default.
• W3112740601 cites W2119608865 @default.
• W3112740601 cites W2125603952 @default.
• W3112740601 cites W2128682770 @default.
• W3112740601 cites W2129272355 @default.
• W3112740601 cites W2144440398 @default.
• W3112740601 cites W2154604162 @default.
• W3112740601 cites W2158708316 @default.
• W3112740601 cites W2158709371 @default.
• W3112740601 cites W2159713856 @default.
• W3112740601 cites W2166779461 @default.
• W3112740601 cites W2419225999 @default.
• W3112740601 cites W2440490401 @default.
• W3112740601 cites W2466795607 @default.
• W3112740601 cites W2512373005 @default.
• W3112740601 cites W2593419960 @default.
• W3112740601 cites W2594398579 @default.
• W3112740601 cites W2611906432 @default.
• W3112740601 cites W2683537437 @default.
• W3112740601 cites W2758840733 @default.
• W3112740601 cites W2805729328 @default.
• W3112740601 cites W2887128430 @default.
• W3112740601 cites W2892309109 @default.
• W3112740601 cites W2894629997 @default.
• W3112740601 cites W2947765278 @default.
• W3112740601 cites W2998788882 @default.
• W3112740601 cites W3007800139 @default.
• W3112740601 cites W3044053922 @default.
• W3112740601 cites W3080430493 @default.
• W3112740601 doi "https://doi.org/10.3390/ijms21249494" @default.
• W3112740601 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7764885" @default.
• W3112740601 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33327444" @default.
• W3112740601 hasPublicationYear "2020" @default.
• W3112740601 type Work @default.
• W3112740601 sameAs 3112740601 @default.
• W3112740601 citedByCount "5" @default.
• W3112740601 countsByYear W31127406012021 @default.
• W3112740601 countsByYear W31127406012022 @default.
• W3112740601 countsByYear W31127406012023 @default.
• W3112740601 crossrefType "journal-article" @default.
• W3112740601 hasAuthorship W3112740601A5002137230 @default.
• W3112740601 hasAuthorship W3112740601A5074065701 @default.
• W3112740601 hasAuthorship W3112740601A5074220233 @default.
• W3112740601 hasBestOaLocation W31127406011 @default.
• W3112740601 hasConcept C100701293 @default.
• W3112740601 hasConcept C104317684 @default.
• W3112740601 hasConcept C105702510 @default.
• W3112740601 hasConcept C2777637488 @default.
• W3112740601 hasConcept C2778022156 @default.
• W3112740601 hasConcept C2779419633 @default.
• W3112740601 hasConcept C3527866 @default.
• W3112740601 hasConcept C3675279 @default.
• W3112740601 hasConcept C38062823 @default.
• W3112740601 hasConcept C523546767 @default.
• W3112740601 hasConcept C54355233 @default.
• W3112740601 hasConcept C55493867 @default.
• W3112740601 hasConcept C59822182 @default.
• W3112740601 hasConcept C67705224 @default.
• W3112740601 hasConcept C67905577 @default.
• W3112740601 hasConcept C86803240 @default.
• W3112740601 hasConcept C88478588 @default.
• W3112740601 hasConcept C89423630 @default.
• W3112740601 hasConcept C95444343 @default.
• W3112740601 hasConceptScore W3112740601C100701293 @default.
• W3112740601 hasConceptScore W3112740601C104317684 @default.

• next