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- W3112796101 abstract "Extracellular matrix (ECM) rigidity has important effects on cell behaviors and increases sharply in liver fibrosis and cirrhosis. Hepatic blood flow is essential in maintaining hepatocytes' (HCs) functions. However, it is still unclear how matrix stiffness and shear stresses orchestrate HC phenotype in concert. A fibrotic three-dimensional (3-D) liver sinusoidal model is constructed using a porous membrane sandwiched between two polydimethylsiloxane (PDMS) layers with respective flow channels. The HCs are cultured in collagen gels of various stiffnesses in the lower channel, whereas the upper channel is pre-seeded with liver sinusoidal endothelial cells (LSECs) and accessible to shear flow. The results reveal that HCs cultured within stiffer matrices exhibit reduced albumin production and cytochrome P450 (CYP450) reductase expression. Low shear stresses enhance synthetic and metabolic functions of HC, whereas high shear stresses lead to the loss of HC phenotype. Furthermore, both mechanical factors regulate HC functions by complementing each other. These observations are likely attributed to mechanically induced mass transport or key signaling molecule of hepatocyte nuclear factor 4α (HNF4α). The present study results provide an insight into understanding the mechanisms of HC dysfunction in liver fibrosis and cirrhosis, especially from the viewpoint of matrix stiffness and blood flow.NEW & NOTEWORTHY A fibrotic three-dimensional (3-D) liver sinusoidal model was constructed to mimic different stages of liver fibrosis in vivo and to explore the cooperative effects of matrix stiffness and shear stresses on hepatocyte (HC) functions. Mechanically induced alterations of mass transport mainly contributed to HC functions via typical mechanosensitive signaling." @default.
- W3112796101 created "2020-12-21" @default.
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- W3112796101 creator A5059556180 @default.
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- W3112796101 date "2021-03-01" @default.
- W3112796101 modified "2023-10-12" @default.
- W3112796101 title "Matrix stiffness and shear stresses modulate hepatocyte functions in a fibrotic liver sinusoidal model" @default.
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- W3112796101 cites W1887589455 @default.
- W3112796101 cites W1967286304 @default.
- W3112796101 cites W1970857673 @default.
- W3112796101 cites W1993417144 @default.
- W3112796101 cites W2000369852 @default.
- W3112796101 cites W2004875910 @default.
- W3112796101 cites W2017695035 @default.
- W3112796101 cites W2023296392 @default.
- W3112796101 cites W2026449906 @default.
- W3112796101 cites W2035516992 @default.
- W3112796101 cites W2039628013 @default.
- W3112796101 cites W2042555336 @default.
- W3112796101 cites W2045055151 @default.
- W3112796101 cites W2045142779 @default.
- W3112796101 cites W2045874791 @default.
- W3112796101 cites W2058642567 @default.
- W3112796101 cites W2061834863 @default.
- W3112796101 cites W2063208689 @default.
- W3112796101 cites W2064341334 @default.
- W3112796101 cites W2064931754 @default.
- W3112796101 cites W2065209626 @default.
- W3112796101 cites W2067916376 @default.
- W3112796101 cites W2068084277 @default.
- W3112796101 cites W2069703095 @default.
- W3112796101 cites W2069819211 @default.
- W3112796101 cites W2074860415 @default.
- W3112796101 cites W2077967017 @default.
- W3112796101 cites W2080325240 @default.
- W3112796101 cites W2089149502 @default.
- W3112796101 cites W2089945888 @default.
- W3112796101 cites W2097969181 @default.
- W3112796101 cites W2098054841 @default.
- W3112796101 cites W2099626081 @default.
- W3112796101 cites W2105872854 @default.
- W3112796101 cites W2121353065 @default.
- W3112796101 cites W2122035828 @default.
- W3112796101 cites W2134522119 @default.
- W3112796101 cites W2146784300 @default.
- W3112796101 cites W2156670210 @default.
- W3112796101 cites W2159897933 @default.
- W3112796101 cites W2161217998 @default.
- W3112796101 cites W2172247815 @default.
- W3112796101 cites W2241161952 @default.
- W3112796101 cites W2302332254 @default.
- W3112796101 cites W2470906679 @default.
- W3112796101 cites W2521901370 @default.
- W3112796101 cites W2570673099 @default.
- W3112796101 cites W2578257435 @default.
- W3112796101 cites W2613180696 @default.
- W3112796101 cites W2613775110 @default.
- W3112796101 cites W2615637577 @default.
- W3112796101 cites W2740525148 @default.
- W3112796101 cites W2768160230 @default.
- W3112796101 cites W2784792741 @default.
- W3112796101 cites W2800163904 @default.
- W3112796101 cites W2804427200 @default.
- W3112796101 cites W2868361013 @default.
- W3112796101 cites W2890493149 @default.
- W3112796101 cites W2895975487 @default.
- W3112796101 cites W2935083370 @default.
- W3112796101 cites W2951686210 @default.
- W3112796101 cites W2954488559 @default.
- W3112796101 cites W2957825823 @default.
- W3112796101 cites W2976809538 @default.
- W3112796101 cites W3003904779 @default.
- W3112796101 cites W4240643054 @default.
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- W3112796101 doi "https://doi.org/10.1152/ajpgi.00379.2019" @default.
- W3112796101 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33296275" @default.
- W3112796101 hasPublicationYear "2021" @default.
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