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• W3113121187 startingPage "461779" @default.
• W3113121187 abstract "We report on the design and performance of in-house built column cartridges that can be directly screwed into the ports of a commercial rotor-stator valve to minimize extra-column band broadening and pressure-drop losses when pursuing ultra-fast separations such as those needed in 2D and 3D-LC separations. Two basic designs were evaluated and were compared with the results obtained with a commercial screw-in column cartridge. The system produces an extra-column band broadening as low as 0.05 to 0.1 μL2 for the employed UV-detector set-up. Despite these very low values, the obtained separation efficiency of the in-house fabricated cartridge columns was very low, corresponding to a reduced minimal plate height around h=7 at the very best, which, for the 1.7 μm particle and 26.4 mm long columns corresponds to a number of theoretical plates of N=2200 under isocratic conditions. A similar poor performance was obtained with a commercial column cartridge with similar dimensions using the same set-up. One possible explanation of the observed performance could be found in the inner diameter of the column cartridges (i.d. =0.75 mm and 1 mm) which, for the employed sub 2-μm particles, falls into a region of column diameters that, according to literature models, is most likely to suffer from inherent packing problems." @default.
• W3113121187 created "2020-12-21" @default.
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• W3113121187 date "2021-01-01" @default.
• W3113121187 modified "2023-10-18" @default.
• W3113121187 title "Column-in-valve designs to minimize extra-column volumes" @default.
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• W3113121187 doi "https://doi.org/10.1016/j.chroma.2020.461779" @default.
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