FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3113166738> ?p ?o ?g. }

• W3113166738 endingPage "4863" @default.
• W3113166738 startingPage "4856" @default.
• W3113166738 abstract "K120 of glycerol 3-phosphate dehydrogenase (GPDH) lies close to the carbonyl group of the bound dihydroxyacetone phosphate (DHAP) dianion. pH rate (pH 4.6-9.0) profiles are reported for kcat and (kcat/Km)dianion for wild type and K120A GPDH-catalyzed reduction of DHAP by NADH, and for (kcat/KdKam) for activation of the variant-catalyzed reduction by CH3CH2NH3+, where Kam and Kd are apparent dissociation constants for CH3CH2NH3+ and DHAP, respectively. These profiles provide evidence that the K120 side chain cation, which is stabilized by an ion-pairing interaction with the D260 side chain, remains protonated between pH 4.6 and 9.0. The profiles for wild type and K120A variant GPDH show downward breaks at a similar pH value (7.6) that are attributed to protonation of the K204 side chain, which also lies close to the substrate carbonyl oxygen. The pH profiles for (kcat/Km)dianion and (kcat/KdKam) for the K120A variant show that the monoprotonated form of the variant is activated for catalysis by CH3CH2NH3+ but has no detectable activity, compared to the diprotonated variant, for unactivated reduction of DHAP. The pH profile for kcat shows that the monoprotonated K120A variant is active toward reduction of enzyme-bound DHAP, because of activation by a ligand-driven conformational change. Upward breaks in the pH profiles for kcat and (kcat/Km)dianion for K120A GPDH are attributed to protonation of D260. These breaks are consistent with the functional replacement of K120 by D260, and a plasticity in the catalytic roles of the active site side chains." @default.
• W3113166738 created "2020-12-21" @default.
• W3113166738 creator A5010726412 @default.
• W3113166738 creator A5018986296 @default.
• W3113166738 creator A5022708392 @default.
• W3113166738 creator A5066402611 @default.
• W3113166738 date "2020-12-11" @default.
• W3113166738 modified "2023-10-14" @default.
• W3113166738 title "Hydride Transfer Catalyzed by Glycerol Phosphate Dehydrogenase: Recruitment of an Acidic Amino Acid Side Chain to Rescue a Damaged Enzyme" @default.
• W3113166738 cites W1543053499 @default.
• W3113166738 cites W1903489927 @default.
• W3113166738 cites W1994376003 @default.
• W3113166738 cites W2003282365 @default.
• W3113166738 cites W2007345034 @default.
• W3113166738 cites W2019564793 @default.
• W3113166738 cites W2023030106 @default.
• W3113166738 cites W2024872256 @default.
• W3113166738 cites W2041372545 @default.
• W3113166738 cites W2067076009 @default.
• W3113166738 cites W2076860230 @default.
• W3113166738 cites W2086494622 @default.
• W3113166738 cites W2109926670 @default.
• W3113166738 cites W2168009444 @default.
• W3113166738 cites W2292814526 @default.
• W3113166738 cites W2324375984 @default.
• W3113166738 cites W2334668751 @default.
• W3113166738 cites W2412617874 @default.
• W3113166738 cites W2546164811 @default.
• W3113166738 cites W2783195348 @default.
• W3113166738 cites W2805210705 @default.
• W3113166738 cites W2808729890 @default.
• W3113166738 cites W2910593503 @default.
• W3113166738 cites W2912594620 @default.
• W3113166738 cites W2951977806 @default.
• W3113166738 cites W3014402767 @default.
• W3113166738 cites W3083426743 @default.
• W3113166738 cites W89774207 @default.
• W3113166738 doi "https://doi.org/10.1021/acs.biochem.0c00801" @default.
• W3113166738 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7784668" @default.
• W3113166738 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33305938" @default.
• W3113166738 hasPublicationYear "2020" @default.
• W3113166738 type Work @default.
• W3113166738 sameAs 3113166738 @default.
• W3113166738 citedByCount "6" @default.
• W3113166738 countsByYear W31131667382021 @default.
• W3113166738 countsByYear W31131667382022 @default.
• W3113166738 crossrefType "journal-article" @default.
• W3113166738 hasAuthorship W3113166738A5010726412 @default.
• W3113166738 hasAuthorship W3113166738A5018986296 @default.
• W3113166738 hasAuthorship W3113166738A5022708392 @default.
• W3113166738 hasAuthorship W3113166738A5066402611 @default.
• W3113166738 hasBestOaLocation W31131667381 @default.
• W3113166738 hasConcept C145148216 @default.
• W3113166738 hasConcept C155647269 @default.
• W3113166738 hasConcept C161790260 @default.
• W3113166738 hasConcept C178790620 @default.
• W3113166738 hasConcept C181199279 @default.
• W3113166738 hasConcept C185592680 @default.
• W3113166738 hasConcept C2776317432 @default.
• W3113166738 hasConcept C2779617269 @default.
• W3113166738 hasConcept C2780866112 @default.
• W3113166738 hasConcept C30095370 @default.
• W3113166738 hasConcept C41183919 @default.
• W3113166738 hasConcept C55493867 @default.
• W3113166738 hasConcept C56856141 @default.
• W3113166738 hasConcept C71240020 @default.
• W3113166738 hasConceptScore W3113166738C145148216 @default.
• W3113166738 hasConceptScore W3113166738C155647269 @default.
• W3113166738 hasConceptScore W3113166738C161790260 @default.
• W3113166738 hasConceptScore W3113166738C178790620 @default.
• W3113166738 hasConceptScore W3113166738C181199279 @default.
• W3113166738 hasConceptScore W3113166738C185592680 @default.
• W3113166738 hasConceptScore W3113166738C2776317432 @default.
• W3113166738 hasConceptScore W3113166738C2779617269 @default.
• W3113166738 hasConceptScore W3113166738C2780866112 @default.
• W3113166738 hasConceptScore W3113166738C30095370 @default.
• W3113166738 hasConceptScore W3113166738C41183919 @default.
• W3113166738 hasConceptScore W3113166738C55493867 @default.
• W3113166738 hasConceptScore W3113166738C56856141 @default.
• W3113166738 hasConceptScore W3113166738C71240020 @default.
• W3113166738 hasFunder F4320337354 @default.
• W3113166738 hasIssue "51" @default.
• W3113166738 hasLocation W31131667381 @default.
• W3113166738 hasLocation W31131667382 @default.
• W3113166738 hasLocation W31131667383 @default.
• W3113166738 hasOpenAccess W3113166738 @default.
• W3113166738 hasPrimaryLocation W31131667381 @default.
• W3113166738 hasRelatedWork W1984539952 @default.
• W3113166738 hasRelatedWork W2004764839 @default.
• W3113166738 hasRelatedWork W2023030106 @default.
• W3113166738 hasRelatedWork W2036861701 @default.
• W3113166738 hasRelatedWork W2051390995 @default.
• W3113166738 hasRelatedWork W2073856318 @default.
• W3113166738 hasRelatedWork W2085450686 @default.
• W3113166738 hasRelatedWork W3113166738 @default.
• W3113166738 hasRelatedWork W4225305107 @default.
• W3113166738 hasRelatedWork W4243895124 @default.
• W3113166738 hasVolume "59" @default.

• next