FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3113366874> ?p ?o ?g. }

• W3113366874 abstract "Abstract Background Retroelements (REs) occupy a significant part of all eukaryotic genomes including humans. The majority of retroelements in the human genome are inactive and unable to retrotranspose. Dozens of active copies are repressed in most normal tissues by various cellular mechanisms. These copies can become active in normal germline and brain tissues or in cancer, leading to new retroposition events. The consequences of such events and their role in normal cell functioning and carcinogenesis are not yet fully understood. If new insertions occur in a small portion of cells they can be found only with the use of specific methods based on RE enrichment and high-throughput sequencing. The downside of the high sensitivity of such methods is the presence of various artifacts imitating real insertions, which in many cases cannot be validated due to lack of the initial template DNA. For this reason, adequate assessment of rare (< 1%) subclonal cancer specific RE insertions is complicated. Results Here we describe a new copy-capture technique which we implemented in a method called SeqURE for Sequencing Unknown of Retroposition Events that allows for efficient and reliable identification of new genomic RE insertions. The method is based on the capture of copies of target molecules (copy-capture), selective amplification and sequencing of genomic regions adjacent to active RE insertions from both sides. Importantly, the template genomic DNA remains intact and can be used for validation experiments. In addition, we applied a novel system for testing method sensitivity and precisely showed the ability of the developed method to reliably detect insertions present in 1 out of 100 cells and a substantial portion of insertions present in 1 out of 1000 cells. Using advantages of the method we showed the absence of somatic Alu insertions in colorectal cancer samples bearing tumor-specific L1HS insertions. Conclusions This study presents the first description and implementation of the copy-capture technique and provides the first methodological basis for the quantitative assessment of RE insertions present in a small portion of cells." @default.
• W3113366874 created "2020-12-21" @default.
• W3113366874 creator A5013138756 @default.
• W3113366874 creator A5013523971 @default.
• W3113366874 creator A5014578289 @default.
• W3113366874 creator A5018769333 @default.
• W3113366874 creator A5026201343 @default.
• W3113366874 creator A5029321368 @default.
• W3113366874 creator A5039561066 @default.
• W3113366874 creator A5052129743 @default.
• W3113366874 creator A5060040008 @default.
• W3113366874 creator A5066151018 @default.
• W3113366874 creator A5066274681 @default.
• W3113366874 creator A5070355358 @default.
• W3113366874 creator A5075957468 @default.
• W3113366874 creator A5085634778 @default.
• W3113366874 date "2020-12-01" @default.
• W3113366874 modified "2023-10-15" @default.
• W3113366874 title "SeqURE – a new copy-capture based method for sequencing of unknown Retroposition events" @default.
• W3113366874 cites W1912672559 @default.
• W3113366874 cites W1970091573 @default.
• W3113366874 cites W1994518469 @default.
• W3113366874 cites W2018177575 @default.
• W3113366874 cites W2032679533 @default.
• W3113366874 cites W2037930474 @default.
• W3113366874 cites W2041677964 @default.
• W3113366874 cites W2044678051 @default.
• W3113366874 cites W2045807857 @default.
• W3113366874 cites W2075224242 @default.
• W3113366874 cites W2091687746 @default.
• W3113366874 cites W2115520939 @default.
• W3113366874 cites W2121996896 @default.
• W3113366874 cites W2123490990 @default.
• W3113366874 cites W2123883764 @default.
• W3113366874 cites W2137607427 @default.
• W3113366874 cites W2154422767 @default.
• W3113366874 cites W2162146849 @default.
• W3113366874 cites W2166993840 @default.
• W3113366874 cites W2169338211 @default.
• W3113366874 cites W2302351081 @default.
• W3113366874 cites W2533759132 @default.
• W3113366874 cites W2579869603 @default.
• W3113366874 cites W2623862783 @default.
• W3113366874 cites W2737372261 @default.
• W3113366874 cites W2760021093 @default.
• W3113366874 cites W2801902861 @default.
• W3113366874 cites W2898777353 @default.
• W3113366874 cites W2971959078 @default.
• W3113366874 cites W2977382078 @default.
• W3113366874 cites W3005218670 @default.
• W3113366874 cites W3008857045 @default.
• W3113366874 cites W3010118112 @default.
• W3113366874 cites W4248538728 @default.
• W3113366874 doi "https://doi.org/10.1186/s13100-020-00228-6" @default.
• W3113366874 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7734759" @default.
• W3113366874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33317630" @default.
• W3113366874 hasPublicationYear "2020" @default.
• W3113366874 type Work @default.
• W3113366874 sameAs 3113366874 @default.
• W3113366874 citedByCount "2" @default.
• W3113366874 countsByYear W31133668742022 @default.
• W3113366874 countsByYear W31133668742023 @default.
• W3113366874 crossrefType "journal-article" @default.
• W3113366874 hasAuthorship W3113366874A5013138756 @default.
• W3113366874 hasAuthorship W3113366874A5013523971 @default.
• W3113366874 hasAuthorship W3113366874A5014578289 @default.
• W3113366874 hasAuthorship W3113366874A5018769333 @default.
• W3113366874 hasAuthorship W3113366874A5026201343 @default.
• W3113366874 hasAuthorship W3113366874A5029321368 @default.
• W3113366874 hasAuthorship W3113366874A5039561066 @default.
• W3113366874 hasAuthorship W3113366874A5052129743 @default.
• W3113366874 hasAuthorship W3113366874A5060040008 @default.
• W3113366874 hasAuthorship W3113366874A5066151018 @default.
• W3113366874 hasAuthorship W3113366874A5066274681 @default.
• W3113366874 hasAuthorship W3113366874A5070355358 @default.
• W3113366874 hasAuthorship W3113366874A5075957468 @default.
• W3113366874 hasAuthorship W3113366874A5085634778 @default.
• W3113366874 hasBestOaLocation W31133668741 @default.
• W3113366874 hasConcept C104317684 @default.
• W3113366874 hasConcept C109825262 @default.
• W3113366874 hasConcept C141231307 @default.
• W3113366874 hasConcept C197077220 @default.
• W3113366874 hasConcept C47042493 @default.
• W3113366874 hasConcept C51679486 @default.
• W3113366874 hasConcept C54355233 @default.
• W3113366874 hasConcept C552990157 @default.
• W3113366874 hasConcept C70721500 @default.
• W3113366874 hasConcept C86803240 @default.
• W3113366874 hasConceptScore W3113366874C104317684 @default.
• W3113366874 hasConceptScore W3113366874C109825262 @default.
• W3113366874 hasConceptScore W3113366874C141231307 @default.
• W3113366874 hasConceptScore W3113366874C197077220 @default.
• W3113366874 hasConceptScore W3113366874C47042493 @default.
• W3113366874 hasConceptScore W3113366874C51679486 @default.
• W3113366874 hasConceptScore W3113366874C54355233 @default.
• W3113366874 hasConceptScore W3113366874C552990157 @default.
• W3113366874 hasConceptScore W3113366874C70721500 @default.
• W3113366874 hasConceptScore W3113366874C86803240 @default.
• W3113366874 hasFunder F4320324099 @default.
• W3113366874 hasIssue "1" @default.

• next