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- W3113449863 abstract "Listeria monocytogenes is a foodborne pathogen of global relevance that causes outbreaks and sporadic cases of listeriosis, acquired through the consumption of contaminated products, including milk or meat products and ready-to-eat meat products subjected to intensive handling. The objective of the present study was to classify L. monocytogenes isolated from various food-related sources in the Federal District of Brazil and surrounding areas to sequence internalin A (inlA) genes from these isolates and assess their adhesion and invasion capacity using Caco-2 cells. In addition, 15 were classified as group I, 3 as group II, and 7 classified as group IV. Premature stop codons (PMSCs) at the nucleotide position 976 (GAA→TAA) of the inlA gene were identified in 5 of the 25 isolates. Adhesion and invasion tests in Caco-2 cells showed that all the isolates were capable of adhesion and cellular invasion, with isolates containing PMSCs exhibiting on average higher invasion capacity than those without PMSCs (p = 0.041) and a median of adhesion very distinctive from those without stop codons. These results are the first report of PMSCs in the inlA gene of L. monocytogenes from the Federal District of Brazil and Brazil." @default.
- W3113449863 created "2021-01-05" @default.
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- W3113449863 date "2021-04-01" @default.
- W3113449863 modified "2023-10-15" @default.
- W3113449863 title "Assessment of Internalin A Gene Sequences and Cell Adhesion and Invasion Capacity of <i>Listeria monocytogenes</i> Strains Isolated from Foods of Animal and Related Origins" @default.
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- W3113449863 doi "https://doi.org/10.1089/fpd.2020.2855" @default.
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