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- W3113669867 abstract "• This study characterized behavioral and molecular responses of Aedes aegypti to ultrasound. • Ultrasound disrupted CO₂-oriented olfactory behaviors of Aedes aegypti . • Long time exposure to ultrasound reduced host finding ability of mosquito. • Ultrasound changed gene expression level of CO 2 receptor and putative hearing related gene. • We suggest that ultrasound can be useful repellent for mosquito control. Mosquito-borne infectious diseases cause mortality and global infectious disease burden worldwide. There are several electronic mosquito repellents (EMRs) based on ultrasound have been developed and commercialized to reduce human-mosquito contacts. However, the efficacy of EMRs against mosquitoes is still unclear. In this study, we present experimental evidence that ultrasound of different frequency and sound pressure differentially affects the host-seeking behavior of Aedes aegypti females. Behavioral tests were accompanied by molecular experiments to check whether mosquitoes respond to ultrasound and are there any changes in specific mRNA expression. Experiments in bioassays revealed that the ultrasound of 100 kHz frequency and 90–110 dB pressure significantly disrupted CO₂-oriented olfactory behaviors and blocked indoor invasion. Furthermore, a long time (>24 h) exposure to 100 kHz frequency/90 dB pressure of ultrasound decreased attractive behaviors to human skin. At the molecular level, there was no change in expression of odorant receptor co-receptor ( AaOrco ) in ultrasound treated animals, while one of the CO 2 receptor genes, AaGr3 , and putative hearing-related gene, AAEL009258 , were down-regulated and up-regulated, respectively. Our study indicates that high frequency (100 kHz) and pressure (90–110 dB) of the ultrasound has repellent effects to olfactory–driven behaviors of mosquitoes." @default.
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- W3113669867 date "2021-04-01" @default.
- W3113669867 modified "2023-09-29" @default.
- W3113669867 title "Behavioral and molecular responses of Aedes aegypti to ultrasound" @default.
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- W3113669867 doi "https://doi.org/10.1016/j.aspen.2020.12.016" @default.
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