FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3113848502> ?p ?o ?g. }

• W3113848502 endingPage "1475" @default.
• W3113848502 startingPage "1475" @default.
• W3113848502 abstract "Flaviviruses bear class II fusion proteins as their envelope (E) proteins. Here, we describe the development of an in vitro quantitative mosquito-cell-based membrane-fusion assay for the E protein using dual split proteins (DSPs). The assay does not involve the use of live viruses and allows the analysis of a membrane-fusion step independent of other events in the viral lifecycle, such as endocytosis. The progress of membrane fusion can be monitored continuously by measuring the activities of Renilla luciferase derived from the reassociation of DSPs during cell fusion. We optimized the assay to screen an FDA-approved drug library for a potential membrane fusion inhibitor using the E protein of Zika virus. Screening results identified atovaquone, which was previously described as an antimalarial agent. Atovaquone potently blocked the in vitro Zika virus infection of mammalian cells with an IC90 of 2.1 µM. Furthermore, four distinct serotypes of dengue virus were also inhibited by atovaquone with IC90 values of 1.6–2.5 µM, which is a range below the average blood concentration of atovaquone after its oral administration in humans. These findings make atovaquone a likely candidate drug to treat illnesses caused by Zika as well as dengue viruses. Additionally, the DSP assay is useful to study the mechanism of membrane fusion in Flaviviruses." @default.
• W3113848502 created "2021-01-05" @default.
• W3113848502 creator A5001285398 @default.
• W3113848502 creator A5007291958 @default.
• W3113848502 creator A5007608926 @default.
• W3113848502 creator A5008391815 @default.
• W3113848502 creator A5026737625 @default.
• W3113848502 creator A5058632940 @default.
• W3113848502 creator A5061857288 @default.
• W3113848502 creator A5062366510 @default.
• W3113848502 creator A5065037360 @default.
• W3113848502 date "2020-12-21" @default.
• W3113848502 modified "2023-10-16" @default.
• W3113848502 title "The Antimalarial Compound Atovaquone Inhibits Zika and Dengue Virus Infection by Blocking E Protein-Mediated Membrane Fusion" @default.
• W3113848502 cites W12260993 @default.
• W3113848502 cites W1557370615 @default.
• W3113848502 cites W1848807523 @default.
• W3113848502 cites W1922608411 @default.
• W3113848502 cites W1991001144 @default.
• W3113848502 cites W1996120355 @default.
• W3113848502 cites W2007561244 @default.
• W3113848502 cites W2029724752 @default.
• W3113848502 cites W2034925881 @default.
• W3113848502 cites W2038504202 @default.
• W3113848502 cites W2048172399 @default.
• W3113848502 cites W2060650602 @default.
• W3113848502 cites W2065906421 @default.
• W3113848502 cites W2079350688 @default.
• W3113848502 cites W2091789910 @default.
• W3113848502 cites W2096025413 @default.
• W3113848502 cites W2098447112 @default.
• W3113848502 cites W2104428288 @default.
• W3113848502 cites W2108687521 @default.
• W3113848502 cites W2112891242 @default.
• W3113848502 cites W2131570682 @default.
• W3113848502 cites W2132645642 @default.
• W3113848502 cites W2139435291 @default.
• W3113848502 cites W2143312620 @default.
• W3113848502 cites W2143312809 @default.
• W3113848502 cites W2145911943 @default.
• W3113848502 cites W2156003470 @default.
• W3113848502 cites W2158336230 @default.
• W3113848502 cites W2168230192 @default.
• W3113848502 cites W2399534185 @default.
• W3113848502 cites W2469258508 @default.
• W3113848502 cites W2499786133 @default.
• W3113848502 cites W2509449660 @default.
• W3113848502 cites W2612436700 @default.
• W3113848502 cites W2767429166 @default.
• W3113848502 cites W2770260204 @default.
• W3113848502 cites W2770282697 @default.
• W3113848502 cites W2771221560 @default.
• W3113848502 cites W2776626262 @default.
• W3113848502 cites W2790432875 @default.
• W3113848502 cites W2792865918 @default.
• W3113848502 cites W2802786085 @default.
• W3113848502 cites W2806918212 @default.
• W3113848502 cites W2897700018 @default.
• W3113848502 cites W2920952239 @default.
• W3113848502 cites W2966347730 @default.
• W3113848502 cites W3033543683 @default.
• W3113848502 cites W622494689 @default.
• W3113848502 doi "https://doi.org/10.3390/v12121475" @default.
• W3113848502 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7767512" @default.
• W3113848502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33371476" @default.
• W3113848502 hasPublicationYear "2020" @default.
• W3113848502 type Work @default.
• W3113848502 sameAs 3113848502 @default.
• W3113848502 citedByCount "8" @default.
• W3113848502 countsByYear W31138485022021 @default.
• W3113848502 countsByYear W31138485022022 @default.
• W3113848502 countsByYear W31138485022023 @default.
• W3113848502 crossrefType "journal-article" @default.
• W3113848502 hasAuthorship W3113848502A5001285398 @default.
• W3113848502 hasAuthorship W3113848502A5007291958 @default.
• W3113848502 hasAuthorship W3113848502A5007608926 @default.
• W3113848502 hasAuthorship W3113848502A5008391815 @default.
• W3113848502 hasAuthorship W3113848502A5026737625 @default.
• W3113848502 hasAuthorship W3113848502A5058632940 @default.
• W3113848502 hasAuthorship W3113848502A5061857288 @default.
• W3113848502 hasAuthorship W3113848502A5062366510 @default.
• W3113848502 hasAuthorship W3113848502A5065037360 @default.
• W3113848502 hasBestOaLocation W31138485021 @default.
• W3113848502 hasConcept C103038307 @default.
• W3113848502 hasConcept C104317684 @default.
• W3113848502 hasConcept C123894998 @default.
• W3113848502 hasConcept C159047783 @default.
• W3113848502 hasConcept C185592680 @default.
• W3113848502 hasConcept C203014093 @default.
• W3113848502 hasConcept C2522874641 @default.
• W3113848502 hasConcept C2777053367 @default.
• W3113848502 hasConcept C2778048844 @default.
• W3113848502 hasConcept C2778371730 @default.
• W3113848502 hasConcept C2779124865 @default.
• W3113848502 hasConcept C2779635636 @default.
• W3113848502 hasConcept C40767141 @default.
• W3113848502 hasConcept C533803919 @default.
• W3113848502 hasConcept C55493867 @default.

• next