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• W3114202489 abstract "We report a highly sensitive approach for detecting microRNA-21 (miR-21) in cancer cells and human serum by using [email protected] nanocomposite labeled lateral flow assay. The [email protected] nanocomposite was prepared by coating numerous 3–5 nm gold nanoparticles (GNP) on a silica nanoparticle (SiNP) with a diameter of 150 nm and used as colored label on the lateral flow assay for signal amplification. TEM results show there are around 1000 GNPs coated on the SiNP surface. The principle of miR-21 detection is based on on-strip DNA-microRNA hybridization reactions to form [email protected] complexes, which are captured on the test zone of the lateral flow test strip and produce a visible red band. A thiol-modified detecting DNA probe (Det-DNA) and a biotin-modified capturing DNA probe (Cap-DNA), which are complementary to miR-21, were used to prepare the lateral flow test strips. After systematic optimization, the method can detect a minimum concentration of 1.0 pM miR-21, which is 60 times lower than that of the GNP-based lateral flow assay (Gao et al. Biosens & Bioelectro, 2014, 54, 578–584). The method was applied to detect miR-21 in cancer cells and spiked human serum with satisfactory results." @default.
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• W3114202489 date "2021-02-01" @default.
• W3114202489 modified "2023-10-06" @default.
• W3114202489 title "Sensitive detection of microRNA-21 in cancer cells and human serum with Au@Si nanocomposite and lateral flow assay" @default.
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• W3114202489 doi "https://doi.org/10.1016/j.aca.2020.12.042" @default.
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