Matches in SemOpenAlex for { <https://semopenalex.org/work/W3114685710> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W3114685710 endingPage "44" @default.
- W3114685710 startingPage "38" @default.
- W3114685710 abstract "Aldehyde-alcohol dehydrogenase (AdhE) is a metabolic enzyme and virulence factor in bacteria. E. coli AdhE (eAdhE) multimerizes into spirosomes that are essential for enzymatic activity. However, it is unknown whether AdhE structure is conserved in divergent bacteria. Here, we present the cryo-EM structure of AdhE (vAdhE) from Vibrio cholerae to 4.31 Å resolution. Overall, vAdhE spirosomes are similar to eAdhE with conserved subunit arrangement. However, divergences in key oligomerization residues cause vAdhE to form labile spirosomes with lower enzymatic activity. Mutating the vAdhE oligomerization interface to mimic eAdhE increases spirosome stability and enzymatic activity to levels comparable to eAdhE. These results support the generality of AdhE spirosome structures, and provide a structural basis to target vAdhE to attenuate bacterial virulence." @default.
- W3114685710 created "2021-01-05" @default.
- W3114685710 creator A5012934386 @default.
- W3114685710 creator A5030966038 @default.
- W3114685710 creator A5051591715 @default.
- W3114685710 creator A5055437860 @default.
- W3114685710 date "2021-01-01" @default.
- W3114685710 modified "2023-10-08" @default.
- W3114685710 title "Cryo-EM structure of Vibrio cholerae aldehyde-alcohol dehydrogenase spirosomes" @default.
- W3114685710 cites W1562537727 @default.
- W3114685710 cites W1676002230 @default.
- W3114685710 cites W1946718856 @default.
- W3114685710 cites W1976965741 @default.
- W3114685710 cites W2001378762 @default.
- W3114685710 cites W2037883986 @default.
- W3114685710 cites W2038287696 @default.
- W3114685710 cites W2060809301 @default.
- W3114685710 cites W2093233573 @default.
- W3114685710 cites W2099540110 @default.
- W3114685710 cites W2132629607 @default.
- W3114685710 cites W2139239302 @default.
- W3114685710 cites W2142449740 @default.
- W3114685710 cites W2155519451 @default.
- W3114685710 cites W2158800792 @default.
- W3114685710 cites W2180229411 @default.
- W3114685710 cites W2181275824 @default.
- W3114685710 cites W2562438459 @default.
- W3114685710 cites W2587625522 @default.
- W3114685710 cites W2593511418 @default.
- W3114685710 cites W2953320394 @default.
- W3114685710 cites W2977547838 @default.
- W3114685710 cites W3012001250 @default.
- W3114685710 cites W3033257239 @default.
- W3114685710 doi "https://doi.org/10.1016/j.bbrc.2020.12.040" @default.
- W3114685710 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33360541" @default.
- W3114685710 hasPublicationYear "2021" @default.
- W3114685710 type Work @default.
- W3114685710 sameAs 3114685710 @default.
- W3114685710 citedByCount "3" @default.
- W3114685710 countsByYear W31146857102021 @default.
- W3114685710 countsByYear W31146857102022 @default.
- W3114685710 countsByYear W31146857102023 @default.
- W3114685710 crossrefType "journal-article" @default.
- W3114685710 hasAuthorship W3114685710A5012934386 @default.
- W3114685710 hasAuthorship W3114685710A5030966038 @default.
- W3114685710 hasAuthorship W3114685710A5051591715 @default.
- W3114685710 hasAuthorship W3114685710A5055437860 @default.
- W3114685710 hasConcept C104292427 @default.
- W3114685710 hasConcept C104317684 @default.
- W3114685710 hasConcept C181199279 @default.
- W3114685710 hasConcept C185592680 @default.
- W3114685710 hasConcept C25747580 @default.
- W3114685710 hasConcept C2777344606 @default.
- W3114685710 hasConcept C2778643871 @default.
- W3114685710 hasConcept C2781090273 @default.
- W3114685710 hasConcept C523546767 @default.
- W3114685710 hasConcept C54355233 @default.
- W3114685710 hasConcept C547475151 @default.
- W3114685710 hasConcept C55493867 @default.
- W3114685710 hasConcept C60987743 @default.
- W3114685710 hasConcept C86803240 @default.
- W3114685710 hasConceptScore W3114685710C104292427 @default.
- W3114685710 hasConceptScore W3114685710C104317684 @default.
- W3114685710 hasConceptScore W3114685710C181199279 @default.
- W3114685710 hasConceptScore W3114685710C185592680 @default.
- W3114685710 hasConceptScore W3114685710C25747580 @default.
- W3114685710 hasConceptScore W3114685710C2777344606 @default.
- W3114685710 hasConceptScore W3114685710C2778643871 @default.
- W3114685710 hasConceptScore W3114685710C2781090273 @default.
- W3114685710 hasConceptScore W3114685710C523546767 @default.
- W3114685710 hasConceptScore W3114685710C54355233 @default.
- W3114685710 hasConceptScore W3114685710C547475151 @default.
- W3114685710 hasConceptScore W3114685710C55493867 @default.
- W3114685710 hasConceptScore W3114685710C60987743 @default.
- W3114685710 hasConceptScore W3114685710C86803240 @default.
- W3114685710 hasFunder F4320322120 @default.
- W3114685710 hasLocation W31146857101 @default.
- W3114685710 hasOpenAccess W3114685710 @default.
- W3114685710 hasPrimaryLocation W31146857101 @default.
- W3114685710 hasRelatedWork W1525975470 @default.
- W3114685710 hasRelatedWork W2017624160 @default.
- W3114685710 hasRelatedWork W2061297944 @default.
- W3114685710 hasRelatedWork W2137846679 @default.
- W3114685710 hasRelatedWork W2143734654 @default.
- W3114685710 hasRelatedWork W2171250717 @default.
- W3114685710 hasRelatedWork W3004503416 @default.
- W3114685710 hasRelatedWork W3114685710 @default.
- W3114685710 hasRelatedWork W4297863195 @default.
- W3114685710 hasRelatedWork W59179329 @default.
- W3114685710 hasVolume "536" @default.
- W3114685710 isParatext "false" @default.
- W3114685710 isRetracted "false" @default.
- W3114685710 magId "3114685710" @default.
- W3114685710 workType "article" @default.