FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3115007109> ?p ?o ?g. }

• W3115007109 endingPage "45" @default.
• W3115007109 startingPage "45" @default.
• W3115007109 abstract "The complement system is involved in promoting secondary injury after traumatic brain injury (TBI), but the roles of the classical and lectin pathways leading to complement activation need to be clarified. To this end, we aimed to determine the ability of the brain to activate the synthesis of classical and lectin pathway initiators in response to TBI and to examine their expression in primary microglial cell cultures. We have modeled TBI in mice by controlled cortical impact (CCI), a clinically relevant experimental model. Using Real-time quantitative polymerase chain reaction (RT-qPCR) we analyzed the expression of initiators of classical the complement component 1q, 1r and 1s (C1q, C1r, and C1s) and lectin (mannose binding lectin A, mannose binding lectin C, collectin 11, ficolin A, and ficolin B) complement pathways and other cellular markers in four brain areas (cortex, striatum, thalamus and hippocampus) of mice exposed to CCI from 24 h and up to 5 weeks. In all murine ipsilateral brain structures assessed, we detected long-lasting, time- and area-dependent significant increases in the mRNA levels of all classical (C1q, C1s, C1r) and some lectin (collectin 11, ficolin A, ficolin B) initiator molecules after TBI. In parallel, we observed significantly enhanced expression of cellular markers for neutrophils (Cd177), T cells (Cd8), astrocytes (glial fibrillary acidic protein—GFAP), microglia/macrophages (allograft inflammatory factor 1—IBA-1), and microglia (transmembrane protein 119—TMEM119); moreover, we detected astrocytes (GFAP) and microglia/macrophages (IBA-1) protein level strong upregulation in all analyzed brain areas. Further, the results obtained in primary microglial cell cultures suggested that these cells may be largely responsible for the biosynthesis of classical pathway initiators. However, microglia are unlikely to be responsible for the production of the lectin pathway initiators. Immunofluorescence analysis confirmed that at the site of brain injury, the C1q is localized in microglia/macrophages and neurons but not in astroglial cells. In sum, the brain strongly reacts to TBI by activating the local synthesis of classical and lectin complement pathway activators. Thus, the brain responds to TBI with a strong, widespread and persistent upregulation of complement components, the targeting of which may provide protection in TBI." @default.
• W3115007109 created "2021-01-05" @default.
• W3115007109 creator A5003101597 @default.
• W3115007109 creator A5006397031 @default.
• W3115007109 creator A5014069149 @default.
• W3115007109 creator A5032167320 @default.
• W3115007109 creator A5034168138 @default.
• W3115007109 creator A5061433194 @default.
• W3115007109 creator A5071920019 @default.
• W3115007109 date "2020-12-22" @default.
• W3115007109 modified "2023-10-17" @default.
• W3115007109 title "Initiators of Classical and Lectin Complement Pathways Are Differently Engaged after Traumatic Brain Injury—Time-Dependent Changes in the Cortex, Striatum, Thalamus and Hippocampus in a Mouse Model" @default.
• W3115007109 cites W1496642521 @default.
• W3115007109 cites W1503617838 @default.
• W3115007109 cites W1504399240 @default.
• W3115007109 cites W1528839689 @default.
• W3115007109 cites W1535546167 @default.
• W3115007109 cites W1547308644 @default.
• W3115007109 cites W1552491055 @default.
• W3115007109 cites W1576335263 @default.
• W3115007109 cites W1599691548 @default.
• W3115007109 cites W1853959720 @default.
• W3115007109 cites W190868370 @default.
• W3115007109 cites W1961991439 @default.
• W3115007109 cites W1968240259 @default.
• W3115007109 cites W1968934499 @default.
• W3115007109 cites W1970275527 @default.
• W3115007109 cites W1977832237 @default.
• W3115007109 cites W1980708340 @default.
• W3115007109 cites W1981953150 @default.
• W3115007109 cites W1984378291 @default.
• W3115007109 cites W1984899716 @default.
• W3115007109 cites W1986341475 @default.
• W3115007109 cites W1987555163 @default.
• W3115007109 cites W1989110124 @default.
• W3115007109 cites W1989331114 @default.
• W3115007109 cites W1991326785 @default.
• W3115007109 cites W1993136341 @default.
• W3115007109 cites W2000463655 @default.
• W3115007109 cites W2000965529 @default.
• W3115007109 cites W2001453076 @default.
• W3115007109 cites W2004509033 @default.
• W3115007109 cites W2005312157 @default.
• W3115007109 cites W2006388578 @default.
• W3115007109 cites W2008386957 @default.
• W3115007109 cites W2011391960 @default.
• W3115007109 cites W2016763491 @default.
• W3115007109 cites W2021336522 @default.
• W3115007109 cites W2022422039 @default.
• W3115007109 cites W2032227357 @default.
• W3115007109 cites W2035408187 @default.
• W3115007109 cites W2036316405 @default.
• W3115007109 cites W2038239633 @default.
• W3115007109 cites W2054275187 @default.
• W3115007109 cites W2056973477 @default.
• W3115007109 cites W2058252406 @default.
• W3115007109 cites W2058595450 @default.
• W3115007109 cites W2064730444 @default.
• W3115007109 cites W2067937957 @default.
• W3115007109 cites W2070111275 @default.
• W3115007109 cites W2071425128 @default.
• W3115007109 cites W2075993625 @default.
• W3115007109 cites W2076087076 @default.
• W3115007109 cites W2079839117 @default.
• W3115007109 cites W2096019298 @default.
• W3115007109 cites W2103693653 @default.
• W3115007109 cites W2119845183 @default.
• W3115007109 cites W2120843520 @default.
• W3115007109 cites W2128517542 @default.
• W3115007109 cites W2140729052 @default.
• W3115007109 cites W2154521199 @default.
• W3115007109 cites W2255138985 @default.
• W3115007109 cites W2280077905 @default.
• W3115007109 cites W2314634699 @default.
• W3115007109 cites W2325050559 @default.
• W3115007109 cites W2337893853 @default.
• W3115007109 cites W2340874950 @default.
• W3115007109 cites W2341088037 @default.
• W3115007109 cites W2342585418 @default.
• W3115007109 cites W2376749222 @default.
• W3115007109 cites W2404046606 @default.
• W3115007109 cites W2469143669 @default.
• W3115007109 cites W2471701648 @default.
• W3115007109 cites W2509415340 @default.
• W3115007109 cites W2511035898 @default.
• W3115007109 cites W2529931034 @default.
• W3115007109 cites W2560250677 @default.
• W3115007109 cites W2567539991 @default.
• W3115007109 cites W2574652423 @default.
• W3115007109 cites W2586825713 @default.
• W3115007109 cites W2594676718 @default.
• W3115007109 cites W2599989119 @default.
• W3115007109 cites W2622909676 @default.
• W3115007109 cites W2728496947 @default.
• W3115007109 cites W2739019370 @default.
• W3115007109 cites W2764063727 @default.
• W3115007109 cites W2769071992 @default.
• W3115007109 cites W2780764893 @default.

• next