FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3115827981> ?p ?o ?g. }

• W3115827981 abstract "β-glucosidase (BglA) immobilization from Thermotoga maritima on magnetic nanoparticles (MNPs) functionalized with chitosan (Cs) were efficiently investigated to improve lactose conversion and galactooligosaccharides (GOS) production. We used a batch method in order to improve the conversion of lactose to GOS. The efficiency and yield of immobilization were 79% and immobilized BglA was effectively recycled via a magnetic separation procedure through a batch-wise GOS with no activity lessening. Furthermore, analyses were done through screening kinetics of enzyme activity, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM). Proposed methodology of immobilization shows a potential application as it is stable which was proved through many methods including pH, temperature, heat treatment, storage, and kinetics of the enzyme. GOS and residual enzyme activity showed to be 28.76 and 40.44%, respectively. However, free enzyme synthesis of GOS yield was just 24% after 12 hr. This study proposed applying magnet in the immobilization process of BglA on Cs-MNPs to produce GOS as new method for immobilizing enzyme in a biostable and cost-efficient way. PRACTICAL APPLICATIONS: This paper focus on immobilization of BglA from T. maritima onto MNPs functionalized with CS to investigate their further possibility improving lactose conversion and GOS production. Interestingly, a successful immobilization of Tm-BglA on the substrates were achieved in Cs-MNPs. The obtained results from enzyme activity, SDS-PAGE, FT-IR, and TEM showed that the high binding capacity of BglA to Cs-MNPs was successfully obtained. Furthermore, the binding efficiency calculation indicated that the immobilized BglA-Cs-MNPs conserved 40.44% of its native activity at the end of its 6th repeated use. In addition, magnetic separation technique was successfully employed for reuse of the immobilized BglA for repetitive batch-wise GOS without significant loss of activity." @default.
• W3115827981 created "2021-01-05" @default.
• W3115827981 creator A5019972776 @default.
• W3115827981 creator A5047514035 @default.
• W3115827981 creator A5059721871 @default.
• W3115827981 creator A5064672070 @default.
• W3115827981 creator A5075766006 @default.
• W3115827981 creator A5079215959 @default.
• W3115827981 creator A5086601917 @default.
• W3115827981 date "2020-12-23" @default.
• W3115827981 modified "2023-10-18" @default.
• W3115827981 title "Large batch production of Galactooligosaccharides using β‐glucosidase immobilized on chitosan‐functionalized magnetic nanoparticle" @default.
• W3115827981 cites W1522715717 @default.
• W3115827981 cites W1963632894 @default.
• W3115827981 cites W1967953658 @default.
• W3115827981 cites W1974667658 @default.
• W3115827981 cites W1974976168 @default.
• W3115827981 cites W1975933303 @default.
• W3115827981 cites W1982350871 @default.
• W3115827981 cites W1986159961 @default.
• W3115827981 cites W1987643693 @default.
• W3115827981 cites W1996612981 @default.
• W3115827981 cites W1998591058 @default.
• W3115827981 cites W2008876346 @default.
• W3115827981 cites W2012484827 @default.
• W3115827981 cites W2014302049 @default.
• W3115827981 cites W2018931215 @default.
• W3115827981 cites W2023843006 @default.
• W3115827981 cites W2027942091 @default.
• W3115827981 cites W2029314103 @default.
• W3115827981 cites W2058989233 @default.
• W3115827981 cites W2062084414 @default.
• W3115827981 cites W2064047263 @default.
• W3115827981 cites W2070282926 @default.
• W3115827981 cites W2072424007 @default.
• W3115827981 cites W2092825009 @default.
• W3115827981 cites W2093293051 @default.
• W3115827981 cites W2097391093 @default.
• W3115827981 cites W2126579209 @default.
• W3115827981 cites W2153427969 @default.
• W3115827981 cites W2155748850 @default.
• W3115827981 cites W2187348079 @default.
• W3115827981 cites W2188263579 @default.
• W3115827981 cites W2332763719 @default.
• W3115827981 cites W2482460093 @default.
• W3115827981 cites W2520199193 @default.
• W3115827981 cites W2536751971 @default.
• W3115827981 cites W2565349909 @default.
• W3115827981 cites W2602949263 @default.
• W3115827981 cites W2788555291 @default.
• W3115827981 cites W2808567897 @default.
• W3115827981 cites W2892617059 @default.
• W3115827981 cites W2912298335 @default.
• W3115827981 cites W3003736535 @default.
• W3115827981 cites W3048770374 @default.
• W3115827981 cites W4253662898 @default.
• W3115827981 cites W73725412 @default.
• W3115827981 doi "https://doi.org/10.1111/jfbc.13589" @default.
• W3115827981 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33368567" @default.
• W3115827981 hasPublicationYear "2020" @default.
• W3115827981 type Work @default.
• W3115827981 sameAs 3115827981 @default.
• W3115827981 citedByCount "5" @default.
• W3115827981 countsByYear W31158279812022 @default.
• W3115827981 countsByYear W31158279812023 @default.
• W3115827981 crossrefType "journal-article" @default.
• W3115827981 hasAuthorship W3115827981A5019972776 @default.
• W3115827981 hasAuthorship W3115827981A5047514035 @default.
• W3115827981 hasAuthorship W3115827981A5059721871 @default.
• W3115827981 hasAuthorship W3115827981A5064672070 @default.
• W3115827981 hasAuthorship W3115827981A5075766006 @default.
• W3115827981 hasAuthorship W3115827981A5079215959 @default.
• W3115827981 hasAuthorship W3115827981A5086601917 @default.
• W3115827981 hasBestOaLocation W31158279811 @default.
• W3115827981 hasConcept C127413603 @default.
• W3115827981 hasConcept C139719470 @default.
• W3115827981 hasConcept C150903083 @default.
• W3115827981 hasConcept C155672457 @default.
• W3115827981 hasConcept C162324750 @default.
• W3115827981 hasConcept C183696295 @default.
• W3115827981 hasConcept C185592680 @default.
• W3115827981 hasConcept C2778348673 @default.
• W3115827981 hasConcept C2779732960 @default.
• W3115827981 hasConcept C31903555 @default.
• W3115827981 hasConcept C42360764 @default.
• W3115827981 hasConcept C55493867 @default.
• W3115827981 hasConcept C86803240 @default.
• W3115827981 hasConcept C87023908 @default.
• W3115827981 hasConceptScore W3115827981C127413603 @default.
• W3115827981 hasConceptScore W3115827981C139719470 @default.
• W3115827981 hasConceptScore W3115827981C150903083 @default.
• W3115827981 hasConceptScore W3115827981C155672457 @default.
• W3115827981 hasConceptScore W3115827981C162324750 @default.
• W3115827981 hasConceptScore W3115827981C183696295 @default.
• W3115827981 hasConceptScore W3115827981C185592680 @default.
• W3115827981 hasConceptScore W3115827981C2778348673 @default.
• W3115827981 hasConceptScore W3115827981C2779732960 @default.
• W3115827981 hasConceptScore W3115827981C31903555 @default.
• W3115827981 hasConceptScore W3115827981C42360764 @default.
• W3115827981 hasConceptScore W3115827981C55493867 @default.

• next