Matches in SemOpenAlex for { <https://semopenalex.org/work/W3116156732> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W3116156732 abstract "Abstract Background: Acetyl-CoA is a fundamental metabolite in Escherichia coli , and also a precursor for biosynthesis of chemicals and materials suitable for multiple applications. The acetyl-CoA synthesis route from glucose presents low atomic economy due to the release of CO 2 in pyruvate decarboxylation reaction. Because ethanol and acetate, both ordinary and inexpensive chemicals, can be converted into acetyl-CoA directly, they could be alternative substrates for production of acetyl-CoA derivatives. Results: In this study, the bifunctional reductase AdhE mutant (A267T/E568K), which converts ethanol into acetyl-CoA, was used to enable E. coli to grow on ethanol, and AMP-forming acetyl-CoA synthetase ACS was employed to enhance the ability of E. coli to utilize acetate. Several products derived from acetyl-CoA, including polyhydroxybutyrate, 3-hydroxypropionate, and phloroglucinol, were produced from glucose, ethanol, and acetate, respectively, by engineered E. coli strains. Compared with glucose and acetate, the strains grown on ethanol presented the highest production and yield of carbon source, and metabolome analysis revealed the reasons of high yield from ethanol. Conclusions: The conversion of ethanol into acetyl-CoA presents high atomic economy along with generation of reducing power, and the yield of target chemical from ethanol is much higher than those from glucose and acetate. All these results suggested that ethanol could be a suitable carbon source for production of acetyl-CoA derived bioproducts." @default.
- W3116156732 created "2021-01-05" @default.
- W3116156732 creator A5007649129 @default.
- W3116156732 creator A5041440753 @default.
- W3116156732 creator A5062253276 @default.
- W3116156732 creator A5068023274 @default.
- W3116156732 creator A5079857642 @default.
- W3116156732 date "2020-03-13" @default.
- W3116156732 modified "2023-09-26" @default.
- W3116156732 title "Comparison of glucose, acetate and ethanol as carbon resource for production of acetyl-CoA derived chemicals in engineered Escherichia coli" @default.
- W3116156732 doi "https://doi.org/10.21203/rs.3.rs-17185/v1" @default.
- W3116156732 hasPublicationYear "2020" @default.
- W3116156732 type Work @default.
- W3116156732 sameAs 3116156732 @default.
- W3116156732 citedByCount "1" @default.
- W3116156732 countsByYear W31161567322020 @default.
- W3116156732 crossrefType "posted-content" @default.
- W3116156732 hasAuthorship W3116156732A5007649129 @default.
- W3116156732 hasAuthorship W3116156732A5041440753 @default.
- W3116156732 hasAuthorship W3116156732A5062253276 @default.
- W3116156732 hasAuthorship W3116156732A5068023274 @default.
- W3116156732 hasAuthorship W3116156732A5079857642 @default.
- W3116156732 hasBestOaLocation W31161567321 @default.
- W3116156732 hasConcept C100544194 @default.
- W3116156732 hasConcept C104317684 @default.
- W3116156732 hasConcept C134121241 @default.
- W3116156732 hasConcept C161790260 @default.
- W3116156732 hasConcept C178790620 @default.
- W3116156732 hasConcept C181199279 @default.
- W3116156732 hasConcept C185592680 @default.
- W3116156732 hasConcept C191897082 @default.
- W3116156732 hasConcept C192562407 @default.
- W3116156732 hasConcept C2778470496 @default.
- W3116156732 hasConcept C2780161600 @default.
- W3116156732 hasConcept C2780595602 @default.
- W3116156732 hasConcept C547475151 @default.
- W3116156732 hasConcept C55493867 @default.
- W3116156732 hasConcept C62231903 @default.
- W3116156732 hasConcept C6350086 @default.
- W3116156732 hasConcept C65183824 @default.
- W3116156732 hasConceptScore W3116156732C100544194 @default.
- W3116156732 hasConceptScore W3116156732C104317684 @default.
- W3116156732 hasConceptScore W3116156732C134121241 @default.
- W3116156732 hasConceptScore W3116156732C161790260 @default.
- W3116156732 hasConceptScore W3116156732C178790620 @default.
- W3116156732 hasConceptScore W3116156732C181199279 @default.
- W3116156732 hasConceptScore W3116156732C185592680 @default.
- W3116156732 hasConceptScore W3116156732C191897082 @default.
- W3116156732 hasConceptScore W3116156732C192562407 @default.
- W3116156732 hasConceptScore W3116156732C2778470496 @default.
- W3116156732 hasConceptScore W3116156732C2780161600 @default.
- W3116156732 hasConceptScore W3116156732C2780595602 @default.
- W3116156732 hasConceptScore W3116156732C547475151 @default.
- W3116156732 hasConceptScore W3116156732C55493867 @default.
- W3116156732 hasConceptScore W3116156732C62231903 @default.
- W3116156732 hasConceptScore W3116156732C6350086 @default.
- W3116156732 hasConceptScore W3116156732C65183824 @default.
- W3116156732 hasLocation W31161567321 @default.
- W3116156732 hasOpenAccess W3116156732 @default.
- W3116156732 hasPrimaryLocation W31161567321 @default.
- W3116156732 hasRelatedWork W1967530400 @default.
- W3116156732 hasRelatedWork W2019588563 @default.
- W3116156732 hasRelatedWork W2103048037 @default.
- W3116156732 hasRelatedWork W2353168268 @default.
- W3116156732 hasRelatedWork W2902918899 @default.
- W3116156732 hasRelatedWork W2956565879 @default.
- W3116156732 hasRelatedWork W3016435528 @default.
- W3116156732 hasRelatedWork W3044996978 @default.
- W3116156732 hasRelatedWork W3107284211 @default.
- W3116156732 hasRelatedWork W3116156732 @default.
- W3116156732 isParatext "false" @default.
- W3116156732 isRetracted "false" @default.
- W3116156732 magId "3116156732" @default.
- W3116156732 workType "article" @default.