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• W3116224725 abstract "Bacterial persistence, where a fraction of a population presents a transient resistance to bactericidal substances, has great medical importance due to its relation with the appearance of antibiotic resistances and untreatable bacterial chronic infections. The mechanisms behind this phenomenon remain largely unknown in spite of recent advances, in great part because of the difficulty in isolating the very small fraction of the population that is in this state at any given time. Current protocols for persister isolation have resulted in possible biases because of the induction of this state by the protocol itself. Here we present a novel protocol that allows rapid isolation of persister cells both from exponential and stationary phase. Moreover, it is capable of differentiating between type I and type II persister cells, which should allow the field to move beyond its current state of studying only one type. While this protocol prompts a revision of many of the current results, it should greatly facilitate further advances in the field. Citation: Canas-Duarte SJ, Restrepo S, Pedraza JM (2014) Novel Protocol for Persister Cells Isolation. PLoS ONE 9(2): e88660. doi:10.1371/journal.pone.0088660 Editor: Eshel Ben-Jacob, Tel Aviv University, Israel Received October 21, 2013; Accepted January 11, 2014; Published February 21, 2014 Copyright: 2014 Canas-Duarte et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: Funded through a ‘‘new assistant professor grant’’ from the University of the Andes. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: jmpedraza@uniandes.edu.co" @default.
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• W3116224725 date "2014-01-01" @default.
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• W3116224725 title "Novel protocol for persister cells isolation" @default.
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