Matches in SemOpenAlex for { <https://semopenalex.org/work/W3116522234> ?p ?o ?g. }
- W3116522234 abstract "Abstract The ability to switch a gene from off to on and monitor dynamic changes provides a powerful approach for probing gene function and elucidating causal regulatory relationships, including instances of feedback control. Here, we developed and characterized YETI (Yeast Estradiol strains with Titratable Induction), a collection in which 5,687 yeast genes are engineered for transcriptional inducibility with single-gene precision at their native loci and without plasmids. Each strain contains Synthetic Genetic Array (SGA) screening markers and a unique molecular barcode, enabling high-throughput yeast genetics. We characterized YETI using quantitative growth phenotyping and pooled BAR-seq screens, and we used a YETI allele to characterize the regulon of ROF1, showing that it is a transcriptional repressor. We observed that strains with inducible essential genes that have low native expression can often grow without inducer. Analysis of data from other eukaryotic and prokaryotic systems shows that low native expression is a critical variable that can bias promoter-perturbing screens, including CRISPRi. We engineered a second expression system, Z 3 EB42, that gives lower expression than Z 3 EV, a feature enabling both conditional activation and repression of lowly expressed essential genes that grow without inducer in the YETI library." @default.
- W3116522234 created "2021-01-05" @default.
- W3116522234 creator A5005009982 @default.
- W3116522234 creator A5007772181 @default.
- W3116522234 creator A5023165801 @default.
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- W3116522234 creator A5077542898 @default.
- W3116522234 creator A5079333329 @default.
- W3116522234 creator A5085220977 @default.
- W3116522234 date "2020-12-30" @default.
- W3116522234 modified "2023-10-11" @default.
- W3116522234 title "A genome-scale yeast library with inducible expression of individual genes" @default.
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