Matches in SemOpenAlex for { <https://semopenalex.org/work/W311698907> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W311698907 abstract "Abstract AIM: To clone and identify the new target genes transactivated by DNAPTP1 of the hepatitis B virus with the suppression subtractive hybridization technique and bioinformatics. METHODS: The mRNA was isolated from HepG2 cells transfected with pcDNA3.1 (-)-DNAPTP1, and then it was transcribed into the cDNA. The pcDNA3.1 (-) empty vector was used as control. After digested by Rsa I, the tester cDNA was then divided into two groups and ligated with two different adaptors. After the tester cDNA was hybridized twice with the driver cDNA and underwent two times of nested polymerase chain reaction (PCR), the amplified cDNA fragments were subcloned into pGEM-Teasy plasmid vectors to construct the subtractive library. The library was amplified by E. coli strain DH5a. The randomly picked cDNA was sequenced and analyzed in the GenBank after the PCR. RESULTS: The cDNA subtractive library of HBV PTP1 transactivating genes was successfully constructed. The amplified library contained 60 positive clones, from which 32 inserts with 200-1 000 bp in length were obtained. Three differentially expressed protein genes and 4 sequences with unknown function were found by sequence analysis." @default.
- W311698907 created "2016-06-24" @default.
- W311698907 creator A5005603340 @default.
- W311698907 creator A5008282602 @default.
- W311698907 creator A5018025210 @default.
- W311698907 creator A5024176882 @default.
- W311698907 creator A5033327065 @default.
- W311698907 creator A5070956450 @default.
- W311698907 date "2005-01-01" @default.
- W311698907 modified "2023-09-27" @default.
- W311698907 title "Cloning of hepatitis B virus DNAPTP1 transactivating genes by suppression subtractive hybridization technique" @default.
- W311698907 cites W1501861737 @default.
- W311698907 cites W1537330292 @default.
- W311698907 cites W1566565153 @default.
- W311698907 cites W1977803316 @default.
- W311698907 cites W1978265006 @default.
- W311698907 cites W1988363595 @default.
- W311698907 cites W1995526187 @default.
- W311698907 cites W1999555354 @default.
- W311698907 cites W2012617920 @default.
- W311698907 cites W2013004044 @default.
- W311698907 cites W2016032631 @default.
- W311698907 cites W2049933997 @default.
- W311698907 cites W2065220670 @default.
- W311698907 cites W2142861964 @default.
- W311698907 cites W2145457002 @default.
- W311698907 doi "https://doi.org/10.11569/wcjd.v13.i19.2371" @default.
- W311698907 hasPublicationYear "2005" @default.
- W311698907 type Work @default.
- W311698907 sameAs 311698907 @default.
- W311698907 citedByCount "0" @default.
- W311698907 crossrefType "journal-article" @default.
- W311698907 hasAuthorship W311698907A5005603340 @default.
- W311698907 hasAuthorship W311698907A5008282602 @default.
- W311698907 hasAuthorship W311698907A5018025210 @default.
- W311698907 hasAuthorship W311698907A5024176882 @default.
- W311698907 hasAuthorship W311698907A5033327065 @default.
- W311698907 hasAuthorship W311698907A5070956450 @default.
- W311698907 hasBestOaLocation W3116989071 @default.
- W311698907 hasConcept C104317684 @default.
- W311698907 hasConcept C121050878 @default.
- W311698907 hasConcept C153911025 @default.
- W311698907 hasConcept C187882448 @default.
- W311698907 hasConcept C189482833 @default.
- W311698907 hasConcept C196436469 @default.
- W311698907 hasConcept C19924922 @default.
- W311698907 hasConcept C199360897 @default.
- W311698907 hasConcept C22744801 @default.
- W311698907 hasConcept C41008148 @default.
- W311698907 hasConcept C49105822 @default.
- W311698907 hasConcept C50724178 @default.
- W311698907 hasConcept C54355233 @default.
- W311698907 hasConcept C79029880 @default.
- W311698907 hasConcept C81089528 @default.
- W311698907 hasConcept C86803240 @default.
- W311698907 hasConceptScore W311698907C104317684 @default.
- W311698907 hasConceptScore W311698907C121050878 @default.
- W311698907 hasConceptScore W311698907C153911025 @default.
- W311698907 hasConceptScore W311698907C187882448 @default.
- W311698907 hasConceptScore W311698907C189482833 @default.
- W311698907 hasConceptScore W311698907C196436469 @default.
- W311698907 hasConceptScore W311698907C19924922 @default.
- W311698907 hasConceptScore W311698907C199360897 @default.
- W311698907 hasConceptScore W311698907C22744801 @default.
- W311698907 hasConceptScore W311698907C41008148 @default.
- W311698907 hasConceptScore W311698907C49105822 @default.
- W311698907 hasConceptScore W311698907C50724178 @default.
- W311698907 hasConceptScore W311698907C54355233 @default.
- W311698907 hasConceptScore W311698907C79029880 @default.
- W311698907 hasConceptScore W311698907C81089528 @default.
- W311698907 hasConceptScore W311698907C86803240 @default.
- W311698907 hasLocation W3116989071 @default.
- W311698907 hasOpenAccess W311698907 @default.
- W311698907 hasPrimaryLocation W3116989071 @default.
- W311698907 hasRelatedWork W1603809388 @default.
- W311698907 hasRelatedWork W2038209199 @default.
- W311698907 hasRelatedWork W2060958215 @default.
- W311698907 hasRelatedWork W2354710845 @default.
- W311698907 hasRelatedWork W2361137867 @default.
- W311698907 hasRelatedWork W2372496536 @default.
- W311698907 hasRelatedWork W2379068255 @default.
- W311698907 hasRelatedWork W2379335831 @default.
- W311698907 hasRelatedWork W2389758373 @default.
- W311698907 hasRelatedWork W2407601533 @default.
- W311698907 isParatext "false" @default.
- W311698907 isRetracted "false" @default.
- W311698907 magId "311698907" @default.
- W311698907 workType "article" @default.