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- W3117433989 abstract "Abstract Background Dedifferentiated fat (DFAT) cells have been studied as a potential tool for various aspects of tissue engineering. However, the understanding of DFAT cells is mainly derived from studies of inguinal subcutaneous or visceral white adipose tissues rather than human bone marrow (BM). Here, we focused on DFAT cells dedifferentiated from bone marrow adipocytes. Methods A plate ceiling culture method and light microscope were used to observe the morphological changes in bone marrow adipocytes. RT-qPCR analysis, immunofluorescence and immunocytochemistry were used to compare gene and protein expression before and after the adipogenic and osteogenic differentiation of BM-DFAT cells and mesenchymal stem cells from bone marrow (BM-MSCs). CCK8 assays and annexin V-PI staining were used to assess the effect of BM-DFAT cells on the proliferation and chemoresistance of leukaemic cells. Results We found that bone marrow adipocytes can dedifferentiate into fibroblast-like multipotent cells, and BM-DFAT cells exhibited similar a morphology, immunophenotype, multipotent gene expression, proliferation ability and osteogenic differentiation in comparison with BM-MSCs. Interestingly, BM-DFAT cells possessed a higher potential for adipogenic differentiation than BM-MSCs, since they expressed a lower level of Pref-1, an inhibitor of adipogenesis. Meanwhile, our results revealed the significantly enhanced proliferation of leukaemic cell lines in the BM-DFAT cell group compared with that in the BM-MSC group. However, the effects of BM-DFAT cells were not as obvious as those of BM-MSCs on the chemoresistance of leukaemic cells. Conclusions This is the first study to illustrate the properties of BM-DFAT cells. We determined that BM-DFAT cells may be a useful cell source to study the role of bone marrow adipocytes and might be utilized for clinical applications in regenerative medicine." @default.
- W3117433989 created "2021-01-05" @default.
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- W3117433989 date "2020-05-21" @default.
- W3117433989 modified "2023-10-13" @default.
- W3117433989 title "Comparison of the properties of bone marrow-derived dedifferentiated fat cells and mesenchymal stem cells in humans" @default.
- W3117433989 doi "https://doi.org/10.21203/rs.3.rs-29140/v1" @default.
- W3117433989 hasPublicationYear "2020" @default.
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