Matches in SemOpenAlex for { <https://semopenalex.org/work/W3117741640> ?p ?o ?g. }
- W3117741640 endingPage "109739" @default.
- W3117741640 startingPage "109739" @default.
- W3117741640 abstract "Lipase from Rhizomucor miehei (RML) was covalently immobilized on different supports, two silica gels and two carbon nanotube samples, using two different strategies. RML was immobilized on 3-carboxypropyl silica gel ([email protected]−COOH) and multi-wall carbon nanotubes containing carboxylic acid functionalities ([email protected]CNT−COOH) using a two-step carbodiimide activation/immobilization reaction. Moreover, the enzyme was also immobilized on 3-aminopropyl silica ([email protected]) and single-wall carbon nanotubes functionalized with 3-APTES and activated with glutaraldehyde ([email protected]). Before and after RML immobilization, the structurel properties of supports were characterized and compared in detail. After immobilization, the expressed activities were 36.9, 90.2, 16.9, and 26.1 % for [email protected]−COOH, [email protected], [email protected]−COOH, and [email protected], respectively. The kinetic parameters of free and immobilized RML samples were determined for three substrates, p-nitrophenyl acetate, p-nitrophenyl butyrate and p-nitrophenyl palmitate, and [email protected] showed higher catalytic efficiency than the other immobilized RML samples. [email protected]−COOH, [email protected], [email protected]−COOH, and [email protected] exhibited 5.8, 7.6, 4.2 and 4.6 folds longer half-life values than those of the free enzyme at pH 7.5 and 40 °C. Recyclability studies showed that all the immobilized RML biocatalysts retained over 90 % of their initial activities after ten cycles in the hydrolysis of p-nitrophenyl butyrate." @default.
- W3117741640 created "2021-01-05" @default.
- W3117741640 creator A5012800479 @default.
- W3117741640 creator A5029195841 @default.
- W3117741640 creator A5039653854 @default.
- W3117741640 creator A5043141080 @default.
- W3117741640 creator A5052047278 @default.
- W3117741640 date "2021-03-01" @default.
- W3117741640 modified "2023-10-18" @default.
- W3117741640 title "Modified silicates and carbon nanotubes for immobilization of lipase from Rhizomucor miehei: Effect of support and immobilization technique on the catalytic performance of the immobilized biocatalysts" @default.
- W3117741640 cites W1959066338 @default.
- W3117741640 cites W1965393877 @default.
- W3117741640 cites W1977477798 @default.
- W3117741640 cites W1977551629 @default.
- W3117741640 cites W1977791017 @default.
- W3117741640 cites W1980461645 @default.
- W3117741640 cites W1987062440 @default.
- W3117741640 cites W1987455712 @default.
- W3117741640 cites W1989621825 @default.
- W3117741640 cites W1989867283 @default.
- W3117741640 cites W1999038254 @default.
- W3117741640 cites W1999825781 @default.
- W3117741640 cites W2001290593 @default.
- W3117741640 cites W2007947135 @default.
- W3117741640 cites W2009349362 @default.
- W3117741640 cites W2011416230 @default.
- W3117741640 cites W2019241354 @default.
- W3117741640 cites W2028181978 @default.
- W3117741640 cites W2028954462 @default.
- W3117741640 cites W2031516270 @default.
- W3117741640 cites W2032614749 @default.
- W3117741640 cites W2038654545 @default.
- W3117741640 cites W2040172877 @default.
- W3117741640 cites W2043451183 @default.
- W3117741640 cites W2046907923 @default.
- W3117741640 cites W2047834391 @default.
- W3117741640 cites W2048243946 @default.
- W3117741640 cites W2055133046 @default.
- W3117741640 cites W2061083538 @default.
- W3117741640 cites W2071764404 @default.
- W3117741640 cites W2072923866 @default.
- W3117741640 cites W2073016734 @default.
- W3117741640 cites W2079013207 @default.
- W3117741640 cites W2081211357 @default.
- W3117741640 cites W2082027989 @default.
- W3117741640 cites W2083984953 @default.
- W3117741640 cites W2085138559 @default.
- W3117741640 cites W2095170400 @default.
- W3117741640 cites W2103454955 @default.
- W3117741640 cites W2112437489 @default.
- W3117741640 cites W2126453940 @default.
- W3117741640 cites W2139071927 @default.
- W3117741640 cites W2145410903 @default.
- W3117741640 cites W2149612297 @default.
- W3117741640 cites W2150847291 @default.
- W3117741640 cites W2152621767 @default.
- W3117741640 cites W2155086483 @default.
- W3117741640 cites W2293910248 @default.
- W3117741640 cites W2305101835 @default.
- W3117741640 cites W2329429905 @default.
- W3117741640 cites W2362753074 @default.
- W3117741640 cites W2398911229 @default.
- W3117741640 cites W2484957945 @default.
- W3117741640 cites W2512784691 @default.
- W3117741640 cites W2531631315 @default.
- W3117741640 cites W2540066272 @default.
- W3117741640 cites W2543766097 @default.
- W3117741640 cites W2570664895 @default.
- W3117741640 cites W2732161821 @default.
- W3117741640 cites W2746868555 @default.
- W3117741640 cites W2764257806 @default.
- W3117741640 cites W2779039214 @default.
- W3117741640 cites W2791209354 @default.
- W3117741640 cites W2792661817 @default.
- W3117741640 cites W2792944996 @default.
- W3117741640 cites W2794044952 @default.
- W3117741640 cites W2794549519 @default.
- W3117741640 cites W2795571603 @default.
- W3117741640 cites W2886922898 @default.
- W3117741640 cites W2887387434 @default.
- W3117741640 cites W2900575710 @default.
- W3117741640 cites W2912869670 @default.
- W3117741640 cites W2913045810 @default.
- W3117741640 cites W2940143329 @default.
- W3117741640 cites W2958048304 @default.
- W3117741640 cites W2968250420 @default.
- W3117741640 cites W2990139425 @default.
- W3117741640 cites W3019314231 @default.
- W3117741640 cites W4293247451 @default.
- W3117741640 doi "https://doi.org/10.1016/j.enzmictec.2020.109739" @default.
- W3117741640 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33541574" @default.
- W3117741640 hasPublicationYear "2021" @default.
- W3117741640 type Work @default.
- W3117741640 sameAs 3117741640 @default.
- W3117741640 citedByCount "24" @default.
- W3117741640 countsByYear W31177416402021 @default.
- W3117741640 countsByYear W31177416402022 @default.
- W3117741640 countsByYear W31177416402023 @default.