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- W3117902905 abstract "Summary The advances in synthetic biology bring exciting new opportunities to reprogram microorganisms with novel functionalities for environmental applications. For real‐world applications, a genetic tool that enables genetic engineering in a stably genomic inherited manner is greatly desired. In this work, we design a novel genetic device for rapid and efficient genome engineering based on the i ntron‐encoded homing‐endonuclease empowered genome editing (iEditing). The iEditing device enables rapid and efficient genome engineering in Shewanella oneidensis MR‐1, the representative strain of the electroactive bacteria group. Moreover, combining with the Red or RecET recombination system, the genome‐editing efficiency was greatly improved, up to approximately 100%. Significantly, the iEditing device itself is eliminated simultaneously when genome editing occurs, thereby requiring no follow‐up to remove the encoding system. Then, we develop a new extracellular electron transfer (EET) engineering strategy by programming the parallel EET systems to enhance versatile EET. The engineered strains exhibit sufficiently enhanced electron output and pollutant reduction ability. Furthermore, this device has demonstrated its great potential to be extended for genome editing in other important microbes. This work provides a useful and efficient tool for the rapid generation of synthetic microorganisms for various environmental applications." @default.
- W3117902905 created "2021-01-05" @default.
- W3117902905 creator A5013233914 @default.
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- W3117902905 creator A5063655609 @default.
- W3117902905 creator A5070716135 @default.
- W3117902905 creator A5073063405 @default.
- W3117902905 creator A5074489920 @default.
- W3117902905 date "2021-01-05" @default.
- W3117902905 modified "2023-10-14" @default.
- W3117902905 title "Rapid and highly efficient genomic engineering with a novel<scp>iEditing</scp>device for programming versatile extracellular electron transfer of electroactive bacteria" @default.
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