FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3118003268> ?p ?o ?g. }

• W3118003268 endingPage "829" @default.
• W3118003268 startingPage "824" @default.
• W3118003268 abstract "Fungi implicated in mycotic keratitis include different species. Conventional methods for the diagnosis of fungal keratitis include staining of corneal scarpings, culture medium (Sabouraud agar) for isolating fungi. Purpose . To evaluate the effectiveness of polymerase chain reaction (PCR) for the detection of fungal etiology in comparison with the conventional diagnostic methods in cases with suspected fungal corneal ulcer. Patients and methods . Seven patients with severe corneal ulcers with more than 3 weeks duration. Corneal scarpings and corneal buttons from seven patients who had undergone therapeutic keratoplasty were used for microbiological and PCR analysis. PCR diagnostic kits for the differential detection of Candida albicans DNA and total fungi DNA (DNA Fungi), which allows to identify most pathogenic fungi without determining their species were used. Microbiological methods: microscopy of gramstained smears, culture techniques, including selective for fungi agar Saburo with chloramphenicol. Results . PCR: Fragments of all corneas removed from keratoplasty (6 patients) revealed fungal-common DNA (Fungi DNA) and did not detect Candida albicans DNA, which correlated with sowing results on Saburo medium (mold fungi found in 5 of 6 corneas). Fungi DNA was also detected in the corneal scraps taken prior to surgery; however, growth of fungi during sowing on various nutrient media was not found. Conclusion . Corneal fungal ulcers are a serious disease, often leading to visual disability. The rapid determination of etiology and the correct choice of therapy determines the outcomes of the disease. The advantage of PCR over the culture method: the speed of obtaining results (4 hours instead of 3–7 days); high sensitivity, which allows detecting fungi not only in the tissue of the removed cornea, but also in scrapes from the cornea ulcer of patients who previously received antifungal therapy. The presence of commercial kits for differential detection of fungal-common DNA and DNA of Candida albicans extends the possibilities of PCR in the screening diagnosis of fungal keratitis and the selection of drugs before determining the type of pathogen." @default.
• W3118003268 created "2021-01-05" @default.
• W3118003268 creator A5018711371 @default.
• W3118003268 creator A5028510784 @default.
• W3118003268 creator A5046457549 @default.
• W3118003268 creator A5046630256 @default.
• W3118003268 creator A5088065340 @default.
• W3118003268 date "2020-12-27" @default.
• W3118003268 modified "2023-10-16" @default.
• W3118003268 title "The Effectiveness of PCR in Diagnosis of Fungal Keratitis" @default.
• W3118003268 cites W1998225942 @default.
• W3118003268 cites W2008876127 @default.
• W3118003268 cites W2012487765 @default.
• W3118003268 cites W2019637910 @default.
• W3118003268 cites W2470059686 @default.
• W3118003268 cites W2614437755 @default.
• W3118003268 cites W2794464626 @default.
• W3118003268 cites W2878095791 @default.
• W3118003268 cites W2903353580 @default.
• W3118003268 cites W2910390683 @default.
• W3118003268 doi "https://doi.org/10.18008/1816-5095-2020-4-824-829" @default.
• W3118003268 hasPublicationYear "2020" @default.
• W3118003268 type Work @default.
• W3118003268 sameAs 3118003268 @default.
• W3118003268 citedByCount "2" @default.
• W3118003268 countsByYear W31180032682021 @default.
• W3118003268 countsByYear W31180032682022 @default.
• W3118003268 crossrefType "journal-article" @default.
• W3118003268 hasAuthorship W3118003268A5018711371 @default.
• W3118003268 hasAuthorship W3118003268A5028510784 @default.
• W3118003268 hasAuthorship W3118003268A5046457549 @default.
• W3118003268 hasAuthorship W3118003268A5046630256 @default.
• W3118003268 hasAuthorship W3118003268A5088065340 @default.
• W3118003268 hasBestOaLocation W31180032681 @default.
• W3118003268 hasConcept C104317684 @default.
• W3118003268 hasConcept C169760540 @default.
• W3118003268 hasConcept C2776079991 @default.
• W3118003268 hasConcept C2776882836 @default.
• W3118003268 hasConcept C2778560704 @default.
• W3118003268 hasConcept C2778660310 @default.
• W3118003268 hasConcept C2780917455 @default.
• W3118003268 hasConcept C3017636755 @default.
• W3118003268 hasConcept C49105822 @default.
• W3118003268 hasConcept C523546767 @default.
• W3118003268 hasConcept C54355233 @default.
• W3118003268 hasConcept C55493867 @default.
• W3118003268 hasConcept C86803240 @default.
• W3118003268 hasConcept C89423630 @default.
• W3118003268 hasConceptScore W3118003268C104317684 @default.
• W3118003268 hasConceptScore W3118003268C169760540 @default.
• W3118003268 hasConceptScore W3118003268C2776079991 @default.
• W3118003268 hasConceptScore W3118003268C2776882836 @default.
• W3118003268 hasConceptScore W3118003268C2778560704 @default.
• W3118003268 hasConceptScore W3118003268C2778660310 @default.
• W3118003268 hasConceptScore W3118003268C2780917455 @default.
• W3118003268 hasConceptScore W3118003268C3017636755 @default.
• W3118003268 hasConceptScore W3118003268C49105822 @default.
• W3118003268 hasConceptScore W3118003268C523546767 @default.
• W3118003268 hasConceptScore W3118003268C54355233 @default.
• W3118003268 hasConceptScore W3118003268C55493867 @default.
• W3118003268 hasConceptScore W3118003268C86803240 @default.
• W3118003268 hasConceptScore W3118003268C89423630 @default.
• W3118003268 hasIssue "4" @default.
• W3118003268 hasLocation W31180032681 @default.
• W3118003268 hasOpenAccess W3118003268 @default.
• W3118003268 hasPrimaryLocation W31180032681 @default.
• W3118003268 hasRelatedWork W168406332 @default.
• W3118003268 hasRelatedWork W1999609690 @default.
• W3118003268 hasRelatedWork W2119924785 @default.
• W3118003268 hasRelatedWork W2188381694 @default.
• W3118003268 hasRelatedWork W2377501261 @default.
• W3118003268 hasRelatedWork W2505994608 @default.
• W3118003268 hasRelatedWork W2560242081 @default.
• W3118003268 hasRelatedWork W2889451064 @default.
• W3118003268 hasRelatedWork W2969526443 @default.
• W3118003268 hasRelatedWork W2181975766 @default.
• W3118003268 hasVolume "17" @default.
• W3118003268 isParatext "false" @default.
• W3118003268 isRetracted "false" @default.
• W3118003268 magId "3118003268" @default.
• W3118003268 workType "article" @default.