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• W3118107305 abstract "HomePlant DiseaseVol. 105, No. 4Cucurbit Leaf Crumple Virus Associated to Watermelon Disease in Campeche State, Mexico PreviousNext DISEASE NOTES OPENOpen Access licenseCucurbit Leaf Crumple Virus Associated to Watermelon Disease in Campeche State, MexicoE. A. Rodríguez-Negrete, R. Jordan-Ramírez, N. E. Leyva-López, and J. Méndez-LozanoE. A. Rodríguez-NegreteCONACyT-Instituto Politécnico Nacional, CIIDIR, Unidad Sinaloa, Departamento de Biotecnología Agrícola, Guasave, Sinaloa, C.P. 81101, MexicoSearch for more papers by this author, R. Jordan-RamírezNunhems BASF Vegetable Seeds, Acampo, CA 95220, U.S.A.Search for more papers by this author, N. E. Leyva-LópezInstituto Politécnico Nacional, CIIDIR, Unidad Sinaloa, Departamento de Biotecnología Agrícola, Guasave, Sinaloa, C.P. 81101, MexicoSearch for more papers by this author, and J. Méndez-Lozano†Corresponding author: J. Méndez-Lozano; E-mail Address: jmendez@ipn.mxhttp://orcid.org/0000-0002-2077-3612Instituto Politécnico Nacional, CIIDIR, Unidad Sinaloa, Departamento de Biotecnología Agrícola, Guasave, Sinaloa, C.P. 81101, MexicoSearch for more papers by this author AffiliationsAuthors and Affiliations E. A. Rodríguez-Negrete1 R. Jordan-Ramírez2 N. E. Leyva-López3 J. Méndez-Lozano3 † 1CONACyT-Instituto Politécnico Nacional, CIIDIR, Unidad Sinaloa, Departamento de Biotecnología Agrícola, Guasave, Sinaloa, C.P. 81101, Mexico 2Nunhems BASF Vegetable Seeds, Acampo, CA 95220, U.S.A. 3Instituto Politécnico Nacional, CIIDIR, Unidad Sinaloa, Departamento de Biotecnología Agrícola, Guasave, Sinaloa, C.P. 81101, Mexico Published Online:29 Mar 2021https://doi.org/10.1094/PDIS-12-20-2551-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleAn annual recurrent disease causing yield reduction in cultivated watermelon (Citrullus lanatus) was documented by the growers in different farms of Campeche state, Mexico. In April 2019 and March 2020, open field grown watermelon plants showed symptoms such as leaf curling, crumpling, and leaf basal or apical necrosis, with an incidence ranging from 30 up to 80%. These plants also presented high populations of whitefly, especially in the most affected fields. To identify the causal agent of the disease, a total of 22 symptomatic watermelon plants were collected in four locations from Campeche state. Total nucleic acids (DNA and RNA) were extracted from these leaf samples. Initially, RT-PCR analysis was performed with specific primers for cucurbit-infecting crinivirus transmitted by whitefly, but the expected size PCR product for those viruses was not amplified in any of these samples. To investigate the presence of cucurbit-infecting begomoviruses, PCR was performed by using specific primers for those begomoviruses reported in Mexico and north/central America, including squash leaf curl virus (SLCV), watermelon chlorotic stunt virus, melon chlorotic leaf curl virus, and cucurbit leaf crumple virus (CuLCrV). Only the expected amplicon size of ∼1,089 bp for CuLCrV was amplified from DNA extracts from all 22 watermelon samples, suggesting a single cucurbit-associated virus. The putative complete genome of the CuLCrV Campeche isolate was amplified by circular DNA enrichment using a rolling circle amplification procedure from two representative samples, followed by enzymatic digestion using BamHI, EcoRI, KpnI, and SacI enzymes (Inoue-Nagata et al. 2004). Expected linearized full-length viral components (∼2.7 kb) were obtained with EcoRI and SacI, and both products, from one selected sample, were cloned into pGreen0029 vector and were fully sequenced. Sequence analysis of the EcoRI clone, designated as LV2019Camp_A (deposited in GenBank as accession no. MW273384) revealed the highest identity of 97.52% to CuLCrV DNA-A Baja California Sur isolate (GenBank accession no. MN625831.1), whereas the KpnI clone, designated as LV2019Camp_B (deposited in GenBank as accession no. MW273385), shared 94.87% identity with DNA B of CuLCrV isolate Arizona (GenBank accession no. AF327559.1). Subsequently, a CuLCrV isolate Campeche-derived agroinfectious clone was obtained by constructing a partial dimeric tandem repeat of both DNA-A and DNA-B components (Bang et al. 2014). Twelve watermelon plants were agroinfiltrated with the infectious clone at the fourth true leaf stage, resulting in symptomatic plants (11/12) exhibiting leaf yellowing, curling, and crumpling 15 days after agroinfiltration, and CuLCrV infection was confirmed by PCR specific detection using DNA extract from noninoculated leaves. Previously, CuLCrV has been detected in the United States (Arizona, Texas, California, Florida, South Carolina, and Georgia) and north Mexico (Coahuila) infecting cucurbits including squash, cucumber, cantaloupe, pumpkin, and watermelon (Brown et al. 2002; Keinath et al. 2018), in both single and mixed infection with other whitefly-transmitted RNA viruses (cucurbit yellow stunting disorder virus, genus Crinivirus) and DNA viruses (SLCV, genus Begomovirus) (Kuo et al. 2007). To our knowledge, this is the first report of CuLCrV infecting a cucurbit crop in the Campeche state from the Yucatán peninsula in Mexico.The author(s) declare no conflict of interest.References:Bang, B., et al. 2014. Plant Pathol. J. 30:310. https://doi.org/10.5423/PPJ.NT.03.2014.0025 Crossref, ISI, Google ScholarBrown, J. K., et al. 2002. Phytopathology 92:734. https://doi.org/10.1094/PHYTO.2002.92.7.734 Link, ISI, Google ScholarInoue-Nagata, A. K., et al. 2004. J. Virol. Methods 116:209. https://doi.org/10.1016/j.jviromet.2003.11.015 Crossref, ISI, Google ScholarKeinath, A. P., et al. 2018. Plant Health Prog. 19:322. https://doi.org/10.1094/PHP-07-18-0039-BR Link, ISI, Google ScholarKuo, Y. W., et al. 2007. Plant Dis. 91:330. https://doi.org/10.1094/PDIS-91-3-0330B Link, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: This work was supported by Instituto Politécnico Nacional and Nunhems de México, S.A. de C.V. through a collaborative project.DetailsFiguresLiterature CitedRelated Vol. 105, No. 4 April 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionLeaf symptom of field-grown ‘Hayward’ kiwifruit plant infected with Actinidia virus (L. Zhao et al.). Photo credit: L. Zhao. Leaf spot of Italian ryegrass naturally infected by Alternaria alternata (X. K. Wei et al.). Photo credit: L. H. Xue. Leaf of Ficus carica displaying symptoms of fig mosaic disease (S. Preising et al.). Photo credit: W. L. da Silva. Metrics Downloaded 929 times Article History Issue Date: 21 Apr 2021Published: 29 Mar 2021First Look: 28 Dec 2020Accepted: 21 Dec 2020 Pages: 1235-1235 Information© 2021 The American Phytopathological SocietyFundingInstituto Politécnico NacionalKeywordsBegomovirusCuLCrVCampecheMexicowatermelonThe author(s) declare no conflict of interest." @default.
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• W3118107305 title "Cucurbit Leaf Crumple Virus Associated to Watermelon Disease in Campeche State, Mexico" @default.
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