Matches in SemOpenAlex for { <https://semopenalex.org/work/W3118256783> ?p ?o ?g. }
- W3118256783 endingPage "263" @default.
- W3118256783 startingPage "263" @default.
- W3118256783 abstract "Breast cancer (BC) metastases to bone disrupt the balance between osteoblasts and osteoclasts, leading to excessive bone resorption. We identified a novel subpopulation of osteoblasts with tumor-inhibitory properties, called educated osteoblasts (EOs). Here we sought to examine the effect of EOs on osteoclastogenesis during tumor progression. We hypothesized that EOs affect osteoclast development in the bone-tumor niche, leading to suppressed pre-osteoclast fusion and bone resorption. Conditioned media (CM) was analyzed for protein expression of osteoclast factors receptor activator of nuclear factor kappa-β ligand (RANKL), osteoprotegerin (OPG), and tumor necrosis factor alpha (TNFα) via ELISA. EOs were co-cultured with pre-osteoclasts on a bone mimetic matrix to assess osteoclast resorption. Pre-osteoclasts were tri-cultured with EOs plus metastatic BC cells and assessed for tartrate-resistance acid phosphatase (TRAP)-positive, multinucleated (≥3 nuclei), mature osteoclasts. Tumor-bearing murine tibias were stained for TRAP to determine osteoclast number in-vivo. EO CM expressed reduced amounts of soluble TNFα and OPG compared to naïve osteoblast CM. Osteoclasts formed in the presence of EOs were smaller and less in number. Upon co-culture on a mimetic bone matrix, a 50% reduction in the number of TRAP-positive osteoclasts formed in the presence of EOs was observed. The tibia of mice inoculated with BC cells had less osteoclasts per bone surface in bones with increased numbers of EO cells. These data suggest EOs reduce osteoclastogenesis and bone resorption. The data imply EOs provide a protective effect against bone resorption in bone metastatic BC." @default.
- W3118256783 created "2021-01-18" @default.
- W3118256783 creator A5003382763 @default.
- W3118256783 creator A5042422898 @default.
- W3118256783 creator A5056820426 @default.
- W3118256783 creator A5061756900 @default.
- W3118256783 date "2021-01-12" @default.
- W3118256783 modified "2023-10-16" @default.
- W3118256783 title "‘Educated’ Osteoblasts Reduce Osteoclastogenesis in a Bone-Tumor Mimetic Microenvironment" @default.
- W3118256783 cites W1594526962 @default.
- W3118256783 cites W1830707738 @default.
- W3118256783 cites W1947259556 @default.
- W3118256783 cites W1966542709 @default.
- W3118256783 cites W1967502057 @default.
- W3118256783 cites W1968362482 @default.
- W3118256783 cites W1968636468 @default.
- W3118256783 cites W1970144926 @default.
- W3118256783 cites W1975154215 @default.
- W3118256783 cites W1975953388 @default.
- W3118256783 cites W1977285029 @default.
- W3118256783 cites W1982915376 @default.
- W3118256783 cites W1985727076 @default.
- W3118256783 cites W1992315247 @default.
- W3118256783 cites W1997865069 @default.
- W3118256783 cites W1998571133 @default.
- W3118256783 cites W2000077738 @default.
- W3118256783 cites W2003213153 @default.
- W3118256783 cites W2005576117 @default.
- W3118256783 cites W2007945453 @default.
- W3118256783 cites W2010908982 @default.
- W3118256783 cites W2014225480 @default.
- W3118256783 cites W2018073425 @default.
- W3118256783 cites W2019146941 @default.
- W3118256783 cites W2025477954 @default.
- W3118256783 cites W2027215054 @default.
- W3118256783 cites W2030565784 @default.
- W3118256783 cites W2037602068 @default.
- W3118256783 cites W2039181986 @default.
- W3118256783 cites W2044878877 @default.
- W3118256783 cites W2046675807 @default.
- W3118256783 cites W2046829147 @default.
- W3118256783 cites W2048299560 @default.
- W3118256783 cites W2059050180 @default.
- W3118256783 cites W2061177902 @default.
- W3118256783 cites W2063699543 @default.
- W3118256783 cites W2064943673 @default.
- W3118256783 cites W2072772987 @default.
- W3118256783 cites W2074906434 @default.
- W3118256783 cites W2078052483 @default.
- W3118256783 cites W2081988588 @default.
- W3118256783 cites W2084202773 @default.
- W3118256783 cites W2084865918 @default.
- W3118256783 cites W2085416266 @default.
- W3118256783 cites W2087324367 @default.
- W3118256783 cites W2088827979 @default.
- W3118256783 cites W2092004666 @default.
- W3118256783 cites W2093252878 @default.
- W3118256783 cites W2093571282 @default.
- W3118256783 cites W2095013915 @default.
- W3118256783 cites W2096425957 @default.
- W3118256783 cites W2096884368 @default.
- W3118256783 cites W2102969047 @default.
- W3118256783 cites W2103885797 @default.
- W3118256783 cites W2106766464 @default.
- W3118256783 cites W2108706718 @default.
- W3118256783 cites W2111239144 @default.
- W3118256783 cites W2123055439 @default.
- W3118256783 cites W2127236332 @default.
- W3118256783 cites W2128520952 @default.
- W3118256783 cites W2131357215 @default.
- W3118256783 cites W2132933661 @default.
- W3118256783 cites W2134457900 @default.
- W3118256783 cites W2136257629 @default.
- W3118256783 cites W2138451733 @default.
- W3118256783 cites W2139996507 @default.
- W3118256783 cites W2142728202 @default.
- W3118256783 cites W2143879648 @default.
- W3118256783 cites W2156789187 @default.
- W3118256783 cites W2158332324 @default.
- W3118256783 cites W2159303972 @default.
- W3118256783 cites W2166509106 @default.
- W3118256783 cites W2170602872 @default.
- W3118256783 cites W2194404144 @default.
- W3118256783 cites W2282302313 @default.
- W3118256783 cites W2321321977 @default.
- W3118256783 cites W2323593510 @default.
- W3118256783 cites W2328859367 @default.
- W3118256783 cites W2330722106 @default.
- W3118256783 cites W2341179483 @default.
- W3118256783 cites W2381577556 @default.
- W3118256783 cites W2413413677 @default.
- W3118256783 cites W2563838328 @default.
- W3118256783 cites W2574362952 @default.
- W3118256783 cites W2612478849 @default.
- W3118256783 cites W2616354594 @default.
- W3118256783 cites W2762443209 @default.
- W3118256783 cites W2771972161 @default.
- W3118256783 cites W2784396775 @default.