Matches in SemOpenAlex for { <https://semopenalex.org/work/W3118505186> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W3118505186 endingPage "S30" @default.
- W3118505186 startingPage "S30" @default.
- W3118505186 abstract "Abstract Background The H. influenzae type b vaccines target the type b capsule and therefore have no impact on the nontypable (unencapsulated) H. influenzae (NTHi). NTHi has become the most common cause of otitis media and is the most common isolate from patients with exacerbations of Chronic Obstructive Pulmonary Disease (COPD). Therefore, NTHi is an appropriate target for vaccine development. Methods To characterize potential vaccine targets, the core outer proteins of NTHi present in the available sequenced genomes were identified through genomic bioinformatics. The structures of the outer proteins were analyzed through comparison with the available structures of homologues characterized by X-ray crystallography. Sequenced conserved outer regions of these proteins were analyzed for their protective capacity in the infant rat model of H. influenzae infection. Results Nine peptides that were protective in the infant rat model were used in a novel vaccine to immunize chinchillas, the most established animal model of otitis media. Chinchillas (40 vaccinated and 41 controls) were infected with NTHi 86-028NP. The vaccinated group cleared infection more quickly than the control group as indicated by significantly decreased positive findings on video-otoscopy (p< 0.0001) and tympanometry (p=0.0002) on day 7, and presence of middle ear fluid obtained by aspiration (p=0.0001) on day 10 post infection. Similarly, in the mouse model of NTHi pulmonary clearance, the vaccinated group (n=5) reduced infection more rapidly than the control group (n= 5), p=0.008. Conclusion These data demonstrate the effectiveness of the Bacterial Vaccine Polypeptide methodology in development of a vaccine against NTHi with protection in relevant preclinical models of both otitis media and pulmonary clearance. The methods are applicable to other bacteria, and this approach to a Bacterial Vaccine Polypeptide against NTHi serves as a paradigm for development of similar vaccines to protect against other bacterial infections. Disclosures All Authors: No reported disclosures" @default.
- W3118505186 created "2021-01-18" @default.
- W3118505186 creator A5013889636 @default.
- W3118505186 creator A5015670403 @default.
- W3118505186 creator A5026541634 @default.
- W3118505186 creator A5071328528 @default.
- W3118505186 creator A5073475539 @default.
- W3118505186 date "2020-10-01" @default.
- W3118505186 modified "2023-09-26" @default.
- W3118505186 title "15. A Novel Approach to Bacterial Vaccines: Haemophilus influenzae as a Paradigm" @default.
- W3118505186 doi "https://doi.org/10.1093/ofid/ofaa439.060" @default.
- W3118505186 hasPublicationYear "2020" @default.
- W3118505186 type Work @default.
- W3118505186 sameAs 3118505186 @default.
- W3118505186 citedByCount "0" @default.
- W3118505186 crossrefType "journal-article" @default.
- W3118505186 hasAuthorship W3118505186A5013889636 @default.
- W3118505186 hasAuthorship W3118505186A5015670403 @default.
- W3118505186 hasAuthorship W3118505186A5026541634 @default.
- W3118505186 hasAuthorship W3118505186A5071328528 @default.
- W3118505186 hasAuthorship W3118505186A5073475539 @default.
- W3118505186 hasBestOaLocation W31185051861 @default.
- W3118505186 hasConcept C126322002 @default.
- W3118505186 hasConcept C141071460 @default.
- W3118505186 hasConcept C159047783 @default.
- W3118505186 hasConcept C203014093 @default.
- W3118505186 hasConcept C2776439223 @default.
- W3118505186 hasConcept C2776925733 @default.
- W3118505186 hasConcept C2992779976 @default.
- W3118505186 hasConcept C501593827 @default.
- W3118505186 hasConcept C71924100 @default.
- W3118505186 hasConcept C86803240 @default.
- W3118505186 hasConcept C89423630 @default.
- W3118505186 hasConceptScore W3118505186C126322002 @default.
- W3118505186 hasConceptScore W3118505186C141071460 @default.
- W3118505186 hasConceptScore W3118505186C159047783 @default.
- W3118505186 hasConceptScore W3118505186C203014093 @default.
- W3118505186 hasConceptScore W3118505186C2776439223 @default.
- W3118505186 hasConceptScore W3118505186C2776925733 @default.
- W3118505186 hasConceptScore W3118505186C2992779976 @default.
- W3118505186 hasConceptScore W3118505186C501593827 @default.
- W3118505186 hasConceptScore W3118505186C71924100 @default.
- W3118505186 hasConceptScore W3118505186C86803240 @default.
- W3118505186 hasConceptScore W3118505186C89423630 @default.
- W3118505186 hasIssue "Supplement_1" @default.
- W3118505186 hasLocation W31185051861 @default.
- W3118505186 hasLocation W31185051862 @default.
- W3118505186 hasLocation W31185051863 @default.
- W3118505186 hasOpenAccess W3118505186 @default.
- W3118505186 hasPrimaryLocation W31185051861 @default.
- W3118505186 hasRelatedWork W1999112956 @default.
- W3118505186 hasRelatedWork W2036699220 @default.
- W3118505186 hasRelatedWork W2042874030 @default.
- W3118505186 hasRelatedWork W2101243222 @default.
- W3118505186 hasRelatedWork W2107069650 @default.
- W3118505186 hasRelatedWork W2152897453 @default.
- W3118505186 hasRelatedWork W2166134351 @default.
- W3118505186 hasRelatedWork W2590399606 @default.
- W3118505186 hasRelatedWork W2917684639 @default.
- W3118505186 hasRelatedWork W386381984 @default.
- W3118505186 hasVolume "7" @default.
- W3118505186 isParatext "false" @default.
- W3118505186 isRetracted "false" @default.
- W3118505186 magId "3118505186" @default.
- W3118505186 workType "article" @default.