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- W3118660690 abstract "A Gram-stain-negative, aerobic, motile, short-rod-shaped bacterium with nicosulfuron-degrading ability, designated strain LAM1902 T , was isolated from a microbial consortium enriched with nicosulfuron as a sole nitrogen and energy source. The optimal temperature and pH for growth of strain LAM1902 T were 30 °C and pH 6.0, respectively. Strain LAM1902 T could grow in the presence of NaCl with concentration up to 4.0 % (w/v). Comparative analysis of 16S rRNA gene sequences revealed that LAM1902 T was closely related to the members of the family Pseudomonadaceae to the genus Pseudomonas , with the highest similarity to Pseudomonas nitroreducens DSM 14399 T (99.6 %), Pseudomonas nitritireducens WZBFD3-5A2 T (99.3 %) and Pseudomonas panipatensis Esp-1 T (98.8 %). Multi-locus sequence analysis based on both concatenated sequences of the 16S rRNA gene and three housekeeping genes ( gyrB , rpoB and rpoD ) further confirmed the intrageneric phylogenetic position of strain LAM1902 T . The genomic DNA G+C content of LAM1902 T was 64.8 mol%. The low values of in silico DNA–DNA hybridization (less than 43.7 %) and average nucleotide identity (less than 90.9 %) also showed that the strain was distinctly different from known species of the genus Pseudomonas . The major fatty acids were C 16 : 0 , C 17 : 0 cyclo and anteiso C 15 : 0 . Ubiquinone Q-9 was detected as the predorminant respiratory quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on phylogenetic, phenotypic and chemotaxonomic analyses and genome comparisons, we conclude that strain LAM1902 T represents a novel species, for which the name Pseudomonas nicosulfuronedens sp. nov. is proposed. The type strain is LAM1902 T (=JCM 33860 T =KCTC 72830 T )." @default.
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- W3118660690 date "2021-02-01" @default.
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- W3118660690 title "Pseudomonas nicosulfuronedens sp. nov., a nicosulfuron degrading bacterium, isolated from a microbial consortium" @default.
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- W3118660690 doi "https://doi.org/10.1099/ijsem.0.004632" @default.
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