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- W3118689635 endingPage "107740" @default.
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- W3118689635 abstract "In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application. Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19–9 (CA19-9), a potential biomarker for endometriosis diagnostics. Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19–9 and exhibited a high sensitivity as (2.55 µA pg−1 cm−1), a detection limit of 0.163 pg mL−1, detection range from 1.0 pg mL−1 to 100 ng mL−1. Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy. To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA). The results of the studies projected AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA. We believe that our fabricated sensor can be the plane of a disease’s management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker." @default.
- W3118689635 created "2021-01-18" @default.
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- W3118689635 date "2021-06-01" @default.
- W3118689635 modified "2023-10-02" @default.
- W3118689635 title "Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application" @default.
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- W3118689635 doi "https://doi.org/10.1016/j.bioelechem.2021.107740" @default.
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