Matches in SemOpenAlex for { <https://semopenalex.org/work/W3118701466> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W3118701466 endingPage "123" @default.
- W3118701466 startingPage "118" @default.
- W3118701466 abstract "Chronic lymphocytic leukemia (CLL) is the most common adult leukaemia in the US and in Europe, including Georgia. CLL presents with clonal expansion and accumulation of CD5+CD19+CD23+ cells in peripheral lymphoid organs and tissues and in bone marrow. The disease remains incurable, albeit there are new molecular and immunotherapy methods currently available, which, in conjunction with chemotherapy, lead to the precision therapy approach. The majority of immunotherapies are based on the ability of therapeutic antibodies to mobilize anti-tumour potential of immune responses. Bispecific antibodies (BsAb) are also considered in the treatment of CLL, whereby phagocytic cells play a key effector role in the destruction of the target CLL cells. Anti-CD19/anti-CD64 BsAb binds to CD19 receptors on CLL cells and to CD64 receptors (FcγRI) on monocytes and activated polymorphonuclear neutrophils (PMNs), thus inducing phagocytosis of the leukaemic cells. The aim of this study was to evaluate the ability of anti-CD19/CD64 BsAb to enhance adherence of CLL cells by PMNs, intact or activated with G-CSF and IFNγ cytokines. Membranes of the isolated CLL cells of 16 patients were stained with Red Fluoresent Linker and CLL cells were co-incubated with isolated autologous PMNs, intact or pre-stimulated with G-CSF and/or IFNγ for 4h or 24h. The PMN/CLL cell adhesion was analyzed with the FACScan flow cytometer by gating on PMNs with adhered RFL-stained CLL cells. The results were heterogenous. Our data demonstrate that anti-CD19/anti-CD64 BsAb has limited capacity to enhance leukemic cell attachment by autologous PMNs. This could partially be explained by the remarkable intensity of spontaneous ability of PMNs to adhere to autologous CLL cells. Pre-treatment of PMNs from CLL patients with G-CSF and INFγ, alone or jointly did not enhance the adhesion of the leukaemic cells. Moreover, G-CSF and IFNγ joint effect led to the reduction in the adhesion capacity of the effector PMNs. It appears, that therapeutic effect of anti-CD19/anti-CD64 BsAb on enhancing attachment of leukaemic cells to PMNs in CLL patients is limited and its application should be based on the assessment of individual capacity of the patients' phagocytic cells." @default.
- W3118701466 created "2021-01-18" @default.
- W3118701466 creator A5026066292 @default.
- W3118701466 creator A5052485795 @default.
- W3118701466 creator A5053225107 @default.
- W3118701466 creator A5063213512 @default.
- W3118701466 creator A5032611927 @default.
- W3118701466 date "2020-11-01" @default.
- W3118701466 modified "2023-09-27" @default.
- W3118701466 title "ATTACHMENT OF CHRONIC LYMPHOCYTIC LEUKAEMIA CELLS BY AUTOLOGOUS POLYMORPHONUCLEAR NEUTROPHILS MEDIATED BY BISPECIFIC ANTI-CD19/CD64 ANTIBODY." @default.
- W3118701466 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33395652" @default.
- W3118701466 hasPublicationYear "2020" @default.
- W3118701466 type Work @default.
- W3118701466 sameAs 3118701466 @default.
- W3118701466 citedByCount "0" @default.
- W3118701466 crossrefType "journal-article" @default.
- W3118701466 hasAuthorship W3118701466A5026066292 @default.
- W3118701466 hasAuthorship W3118701466A5032611927 @default.
- W3118701466 hasAuthorship W3118701466A5052485795 @default.
- W3118701466 hasAuthorship W3118701466A5053225107 @default.
- W3118701466 hasAuthorship W3118701466A5063213512 @default.
- W3118701466 hasConcept C10205521 @default.
- W3118701466 hasConcept C113067387 @default.
- W3118701466 hasConcept C159654299 @default.
- W3118701466 hasConcept C159912055 @default.
- W3118701466 hasConcept C203014093 @default.
- W3118701466 hasConcept C2776447464 @default.
- W3118701466 hasConcept C2777938653 @default.
- W3118701466 hasConcept C2778461978 @default.
- W3118701466 hasConcept C2778957590 @default.
- W3118701466 hasConcept C2780007613 @default.
- W3118701466 hasConcept C28328180 @default.
- W3118701466 hasConcept C502942594 @default.
- W3118701466 hasConcept C54355233 @default.
- W3118701466 hasConcept C71924100 @default.
- W3118701466 hasConcept C86803240 @default.
- W3118701466 hasConcept C8891405 @default.
- W3118701466 hasConceptScore W3118701466C10205521 @default.
- W3118701466 hasConceptScore W3118701466C113067387 @default.
- W3118701466 hasConceptScore W3118701466C159654299 @default.
- W3118701466 hasConceptScore W3118701466C159912055 @default.
- W3118701466 hasConceptScore W3118701466C203014093 @default.
- W3118701466 hasConceptScore W3118701466C2776447464 @default.
- W3118701466 hasConceptScore W3118701466C2777938653 @default.
- W3118701466 hasConceptScore W3118701466C2778461978 @default.
- W3118701466 hasConceptScore W3118701466C2778957590 @default.
- W3118701466 hasConceptScore W3118701466C2780007613 @default.
- W3118701466 hasConceptScore W3118701466C28328180 @default.
- W3118701466 hasConceptScore W3118701466C502942594 @default.
- W3118701466 hasConceptScore W3118701466C54355233 @default.
- W3118701466 hasConceptScore W3118701466C71924100 @default.
- W3118701466 hasConceptScore W3118701466C86803240 @default.
- W3118701466 hasConceptScore W3118701466C8891405 @default.
- W3118701466 hasIssue "308" @default.
- W3118701466 hasLocation W31187014661 @default.
- W3118701466 hasOpenAccess W3118701466 @default.
- W3118701466 hasPrimaryLocation W31187014661 @default.
- W3118701466 hasRelatedWork W1809040828 @default.
- W3118701466 hasRelatedWork W1964899672 @default.
- W3118701466 hasRelatedWork W2042155937 @default.
- W3118701466 hasRelatedWork W2045531733 @default.
- W3118701466 hasRelatedWork W2093394631 @default.
- W3118701466 hasRelatedWork W2116555417 @default.
- W3118701466 hasRelatedWork W2157010102 @default.
- W3118701466 hasRelatedWork W2165723917 @default.
- W3118701466 hasRelatedWork W2165814241 @default.
- W3118701466 hasRelatedWork W253201866 @default.
- W3118701466 hasRelatedWork W2556836201 @default.
- W3118701466 hasRelatedWork W2577435188 @default.
- W3118701466 hasRelatedWork W2582798581 @default.
- W3118701466 hasRelatedWork W2783257882 @default.
- W3118701466 hasRelatedWork W2800496748 @default.
- W3118701466 hasRelatedWork W2990103834 @default.
- W3118701466 hasRelatedWork W3025899236 @default.
- W3118701466 hasRelatedWork W3044178710 @default.
- W3118701466 hasRelatedWork W3159426888 @default.
- W3118701466 hasRelatedWork W3169984771 @default.
- W3118701466 isParatext "false" @default.
- W3118701466 isRetracted "false" @default.
- W3118701466 magId "3118701466" @default.
- W3118701466 workType "article" @default.