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• W3118823687 abstract "The early monitoring of TNF-α has important clinical significance for newborn disease screening. Therefore, a reusable electrochemical aptasensor based on CeO2@Au nanorods-PAMAM-ferrocene (Ce@AuNRs-PAMAM-Fc) and enzyme-free DNA walker technology was developed for the ultrasensitive detection of TNF-α. To amplify the signal, Ce@AuNRs was prepared by in situ synthesis of gold nanoparticles on the surface of CeO2NRs and connected with PAMAM-COOH and Fc-COOH to form Ce@AuNRs-PAMAM-Fc. As signal tags, Ce@AuNRs-PAMAM-Fc could catalyze the redox reaction of H2O2 with the changes in current signals. In addition, the enzyme-free DNA walker technology was used to continuously cleave the signal probe to achieve the dual-amplification of the signal. The developed aptasensor could detect TNF-α in the range of 2 to 2 × 106 pg/mL, with a detection limit of 0.6 pg/mL. It is worth mentioning that the combination of DNA walker and strand displacement technology made the aptasensor reused up to 7 times, greatly reducing the detection cost. In summary, the highly sensitive and reusable aptasensor is a promising strategy for the early screening of neonatal diseases." @default.
• W3118823687 created "2021-01-18" @default.
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• W3118823687 date "2021-01-06" @default.
• W3118823687 modified "2023-09-23" @default.
• W3118823687 title "A reusable aptasensor based on the dual signal amplification of Ce@AuNRs-PAMAM-Fc and DNA walker for ultrasensitive detection of TNF-α" @default.
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• W3118823687 doi "https://doi.org/10.1007/s10008-020-04885-8" @default.
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