Matches in SemOpenAlex for { <https://semopenalex.org/work/W3118905024> ?p ?o ?g. }
- W3118905024 endingPage "43" @default.
- W3118905024 startingPage "34" @default.
- W3118905024 abstract "Induced pluripotent stem cells (iPSCs) hold a great potential for therapeutic regenerative medicine. The aim of this study was to generate induced pluripotent stem cells from goat embryonic cardiac tissue derived fibroblasts. The isolated cardiac fibroblasts from the cardiac tissue of goat embryos were positive for alfa smooth muscle actin, vimentin and discoidin domain receptor2. From these cells, we generated transgene free iPSCs using piggyBac transposons / transposase using five transcription factors (Oct4, Sox2, Klf, Myc and Lin 28). The generated iPSCs were SSEA1, SSEA4 and Oct4 positive. They were cultured on neofeeders using 20% Serum replacement - IMDM with bFGF. They could form cystic and compact embryoid bodies that showed differentiated ectodermal and mesodermal like cells when cultured using 20% FBS-IMDM without bFGF. The iPSCs, generated in the frame of this approach were produced without the use of integrating virus and the reprogramming transgenes were removed at the end of the process. Though there were limitations in the approach used, a substantial sign of reprogramming was obtained." @default.
- W3118905024 created "2021-01-18" @default.
- W3118905024 creator A5030757839 @default.
- W3118905024 creator A5061815033 @default.
- W3118905024 creator A5063295638 @default.
- W3118905024 creator A5063495799 @default.
- W3118905024 creator A5073128785 @default.
- W3118905024 creator A5076912351 @default.
- W3118905024 creator A5083746732 @default.
- W3118905024 date "2020-12-11" @default.
- W3118905024 modified "2023-10-10" @default.
- W3118905024 title "Generation of transgene-free induced pluripotent stem cells from cardiac fibroblasts of goat embryos" @default.
- W3118905024 cites W1983935256 @default.
- W3118905024 cites W1997323395 @default.
- W3118905024 cites W2004368167 @default.
- W3118905024 cites W2004929098 @default.
- W3118905024 cites W2006878971 @default.
- W3118905024 cites W2008566712 @default.
- W3118905024 cites W2014916380 @default.
- W3118905024 cites W2016140632 @default.
- W3118905024 cites W2021581968 @default.
- W3118905024 cites W2027952618 @default.
- W3118905024 cites W2028103767 @default.
- W3118905024 cites W2030983339 @default.
- W3118905024 cites W2036324628 @default.
- W3118905024 cites W2043253996 @default.
- W3118905024 cites W2056413676 @default.
- W3118905024 cites W2064681350 @default.
- W3118905024 cites W2064731864 @default.
- W3118905024 cites W2065901519 @default.
- W3118905024 cites W2075136524 @default.
- W3118905024 cites W2078804410 @default.
- W3118905024 cites W2081331532 @default.
- W3118905024 cites W2106485710 @default.
- W3118905024 cites W2108590496 @default.
- W3118905024 cites W2109168637 @default.
- W3118905024 cites W2109774918 @default.
- W3118905024 cites W2114022429 @default.
- W3118905024 cites W2117156910 @default.
- W3118905024 cites W2125987139 @default.
- W3118905024 cites W2129328611 @default.
- W3118905024 cites W2129660202 @default.
- W3118905024 cites W2136125227 @default.
- W3118905024 cites W2136404196 @default.
- W3118905024 cites W2137283360 @default.
- W3118905024 cites W2138575600 @default.
- W3118905024 cites W2138977668 @default.
- W3118905024 cites W2141050718 @default.
- W3118905024 cites W2142046859 @default.
- W3118905024 cites W2147921164 @default.
- W3118905024 cites W2151977481 @default.
- W3118905024 cites W2153162499 @default.
- W3118905024 cites W2157805620 @default.
- W3118905024 cites W2158771952 @default.
- W3118905024 cites W2158979552 @default.
- W3118905024 cites W2160824531 @default.
- W3118905024 cites W2161923979 @default.
- W3118905024 cites W2163388642 @default.
- W3118905024 cites W2415526055 @default.
- W3118905024 cites W2516450226 @default.
- W3118905024 cites W2770891066 @default.
- W3118905024 doi "https://doi.org/10.46582/jsrm.1602007" @default.
- W3118905024 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7772807" @default.
- W3118905024 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33414579" @default.
- W3118905024 hasPublicationYear "2020" @default.
- W3118905024 type Work @default.
- W3118905024 sameAs 3118905024 @default.
- W3118905024 citedByCount "3" @default.
- W3118905024 countsByYear W31189050242023 @default.
- W3118905024 crossrefType "journal-article" @default.
- W3118905024 hasAuthorship W3118905024A5030757839 @default.
- W3118905024 hasAuthorship W3118905024A5061815033 @default.
- W3118905024 hasAuthorship W3118905024A5063295638 @default.
- W3118905024 hasAuthorship W3118905024A5063495799 @default.
- W3118905024 hasAuthorship W3118905024A5073128785 @default.
- W3118905024 hasAuthorship W3118905024A5076912351 @default.
- W3118905024 hasAuthorship W3118905024A5083746732 @default.
- W3118905024 hasBestOaLocation W31189050241 @default.
- W3118905024 hasConcept C102230213 @default.
- W3118905024 hasConcept C104317684 @default.
- W3118905024 hasConcept C107459253 @default.
- W3118905024 hasConcept C145103041 @default.
- W3118905024 hasConcept C196843134 @default.
- W3118905024 hasConcept C28328180 @default.
- W3118905024 hasConcept C54355233 @default.
- W3118905024 hasConcept C86803240 @default.
- W3118905024 hasConcept C95444343 @default.
- W3118905024 hasConceptScore W3118905024C102230213 @default.
- W3118905024 hasConceptScore W3118905024C104317684 @default.
- W3118905024 hasConceptScore W3118905024C107459253 @default.
- W3118905024 hasConceptScore W3118905024C145103041 @default.
- W3118905024 hasConceptScore W3118905024C196843134 @default.
- W3118905024 hasConceptScore W3118905024C28328180 @default.
- W3118905024 hasConceptScore W3118905024C54355233 @default.
- W3118905024 hasConceptScore W3118905024C86803240 @default.
- W3118905024 hasConceptScore W3118905024C95444343 @default.
- W3118905024 hasIssue "2" @default.
- W3118905024 hasLocation W31189050241 @default.