Matches in SemOpenAlex for { <https://semopenalex.org/work/W3119005688> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W3119005688 endingPage "736338" @default.
- W3119005688 startingPage "736338" @default.
- W3119005688 abstract "We established a recombinase polymerase amplification (RPA) assay for the rapid detection of viral hemorrhagic septicemia virus (VHSV) using primers designed to the N gene in this species. Optimization experiments showed that the optimal amplification temperature of the RPA assay was 37 °C and that the reaction could be completed within only 15 min. Specificity tests showed that the RPA assay did not exhibit any cross-reactivity with other infective fish viruses. Sensitivity tests further showed that the detection limit of the new RPA assay was 8.3 copies/μL, indicating that this assay was more sensitive than traditional RT-PCR method. The method was verified by detecting VHSV in 1924 batches of samples collected from domestic and imported fishes. The detection results were consistent with that of traditional RT-PCR, and the specificity and sensitivity of the method met the detection requirements for aquatic animal diseases. Collectively, our findings indicate that the novel RPA assay is fast, simple, specific, sensitive, and has significant potential for the clinic, on-site testing, and a wide range of other applications." @default.
- W3119005688 created "2021-01-18" @default.
- W3119005688 creator A5003048872 @default.
- W3119005688 creator A5006395886 @default.
- W3119005688 creator A5008545164 @default.
- W3119005688 creator A5048461054 @default.
- W3119005688 creator A5070330941 @default.
- W3119005688 creator A5072550199 @default.
- W3119005688 creator A5073622988 @default.
- W3119005688 creator A5088067833 @default.
- W3119005688 date "2021-03-01" @default.
- W3119005688 modified "2023-09-27" @default.
- W3119005688 title "Establishment of recombinase polymerase amplification technology for detecting Viral Hemorrhagic Septicemia Virus (VHSV) in cultured aquatic animals" @default.
- W3119005688 cites W1997907354 @default.
- W3119005688 cites W2007474216 @default.
- W3119005688 cites W2015469258 @default.
- W3119005688 cites W2034138202 @default.
- W3119005688 cites W2037478973 @default.
- W3119005688 cites W2051177688 @default.
- W3119005688 cites W2150598751 @default.
- W3119005688 cites W2153350094 @default.
- W3119005688 cites W2166724355 @default.
- W3119005688 cites W2257499382 @default.
- W3119005688 cites W2328480348 @default.
- W3119005688 cites W2766989075 @default.
- W3119005688 cites W2272785270 @default.
- W3119005688 doi "https://doi.org/10.1016/j.aquaculture.2020.736338" @default.
- W3119005688 hasPublicationYear "2021" @default.
- W3119005688 type Work @default.
- W3119005688 sameAs 3119005688 @default.
- W3119005688 citedByCount "1" @default.
- W3119005688 countsByYear W31190056882022 @default.
- W3119005688 crossrefType "journal-article" @default.
- W3119005688 hasAuthorship W3119005688A5003048872 @default.
- W3119005688 hasAuthorship W3119005688A5006395886 @default.
- W3119005688 hasAuthorship W3119005688A5008545164 @default.
- W3119005688 hasAuthorship W3119005688A5048461054 @default.
- W3119005688 hasAuthorship W3119005688A5070330941 @default.
- W3119005688 hasAuthorship W3119005688A5072550199 @default.
- W3119005688 hasAuthorship W3119005688A5073622988 @default.
- W3119005688 hasAuthorship W3119005688A5088067833 @default.
- W3119005688 hasConcept C102414288 @default.
- W3119005688 hasConcept C104317684 @default.
- W3119005688 hasConcept C119128265 @default.
- W3119005688 hasConcept C139713532 @default.
- W3119005688 hasConcept C153911025 @default.
- W3119005688 hasConcept C156695909 @default.
- W3119005688 hasConcept C159047783 @default.
- W3119005688 hasConcept C185592680 @default.
- W3119005688 hasConcept C2522874641 @default.
- W3119005688 hasConcept C43617362 @default.
- W3119005688 hasConcept C48023723 @default.
- W3119005688 hasConcept C49105822 @default.
- W3119005688 hasConcept C54355233 @default.
- W3119005688 hasConcept C86803240 @default.
- W3119005688 hasConceptScore W3119005688C102414288 @default.
- W3119005688 hasConceptScore W3119005688C104317684 @default.
- W3119005688 hasConceptScore W3119005688C119128265 @default.
- W3119005688 hasConceptScore W3119005688C139713532 @default.
- W3119005688 hasConceptScore W3119005688C153911025 @default.
- W3119005688 hasConceptScore W3119005688C156695909 @default.
- W3119005688 hasConceptScore W3119005688C159047783 @default.
- W3119005688 hasConceptScore W3119005688C185592680 @default.
- W3119005688 hasConceptScore W3119005688C2522874641 @default.
- W3119005688 hasConceptScore W3119005688C43617362 @default.
- W3119005688 hasConceptScore W3119005688C48023723 @default.
- W3119005688 hasConceptScore W3119005688C49105822 @default.
- W3119005688 hasConceptScore W3119005688C54355233 @default.
- W3119005688 hasConceptScore W3119005688C86803240 @default.
- W3119005688 hasFunder F4320335777 @default.
- W3119005688 hasLocation W31190056881 @default.
- W3119005688 hasOpenAccess W3119005688 @default.
- W3119005688 hasPrimaryLocation W31190056881 @default.
- W3119005688 hasRelatedWork W1938450991 @default.
- W3119005688 hasRelatedWork W2054848430 @default.
- W3119005688 hasRelatedWork W2056420846 @default.
- W3119005688 hasRelatedWork W2070179873 @default.
- W3119005688 hasRelatedWork W2086839033 @default.
- W3119005688 hasRelatedWork W2147456190 @default.
- W3119005688 hasRelatedWork W2160158790 @default.
- W3119005688 hasRelatedWork W2801487330 @default.
- W3119005688 hasRelatedWork W3140247098 @default.
- W3119005688 hasRelatedWork W4256666005 @default.
- W3119005688 hasVolume "535" @default.
- W3119005688 isParatext "false" @default.
- W3119005688 isRetracted "false" @default.
- W3119005688 magId "3119005688" @default.
- W3119005688 workType "article" @default.