FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3119043322> ?p ?o ?g. }

• W3119043322 abstract "Abstract Sandalwood ( Santalum album L.) is highly valued for its fragrant heartwood and extracted oil. Santalols, which are the main components of that oil, are terpenoids, and these are biosynthesized via the mevalonic acid (MVA) pathway. Mevalonate kinase (MK) and phosphomevalonate kinase (PMK) are key enzymes in the MVA pathway. Little is known about the genes that encode MK and PMK in S. album or the mechanism that regulates their expression. To isolate and identify the functional genes involved in santalol biosynthesis in S. album , an MK gene designated as SaMK , and a PMK gene designated as SaPMK , were cloned from S. album . The sequences of these genes were analyzed. A bioinformatics analysis was conducted to assess the homology of SaMK and SaPMK with MK and PMK genes from other plants. The subcellular localization of SaMK and SaPMK proteins was also investigated, as was the functional complementation of SaMK and SaPMK in yeast. Our results show that the full-length cDNA sequences of SaMK and SaPMK were 1409 bp and 1679 bp long, respectively. SaMK contained a 1381 bp open reading frame (ORF) encoding a polypeptide of 460 amino acids and SaPMK contained a 1527 bp ORF encoding a polypeptide of 508 amino acids. SaMK and SaPMK showed high homology with MK and PMK genes of other plant species. Functional complementation of SaMK in a MK-deficient mutant yeast strain YMR208W and SaPMK in a PMK-deficient mutant yeast strain YMR220W confirmed that cloned SaMK and SaPMK cDNA encode a functional MK and PMK, respectively, mediating MVA biosynthesis in yeast. An analysis of tissue expression patterns revealed that SaMK and SaPMK were constitutively expressed in all the tested tissues. SaMK was highly expressed in young leaves but weakly expressed in sapwood. SaPMK was highly expressed in roots and mature leaves, but weakly expressed in young leaves. Induction experiments with several elicitors showed that SaMK and SaPMK expression was upregulated by methyl jasmonate. These results will help to further study the role of MK and PMK genes during santalol biosynthesis in S. album ." @default.
• W3119043322 created "2021-01-18" @default.
• W3119043322 creator A5020797341 @default.
• W3119043322 creator A5038911681 @default.
• W3119043322 creator A5041152613 @default.
• W3119043322 creator A5060243216 @default.
• W3119043322 creator A5067022501 @default.
• W3119043322 creator A5087016011 @default.
• W3119043322 creator A5089372948 @default.
• W3119043322 date "2021-08-19" @default.
• W3119043322 modified "2023-10-14" @default.
• W3119043322 title "Cloning and expression analysis of mevalonate kinase and phosphomevalonate kinase genes associated with the MVA pathway in Santalum album" @default.
• W3119043322 cites W1498633645 @default.
• W3119043322 cites W1544160864 @default.
• W3119043322 cites W1569256603 @default.
• W3119043322 cites W1815802714 @default.
• W3119043322 cites W1969914292 @default.
• W3119043322 cites W1973360750 @default.
• W3119043322 cites W1974090791 @default.
• W3119043322 cites W1976344731 @default.
• W3119043322 cites W1978268205 @default.
• W3119043322 cites W1988583122 @default.
• W3119043322 cites W1989009247 @default.
• W3119043322 cites W1989060614 @default.
• W3119043322 cites W1989656484 @default.
• W3119043322 cites W1994101778 @default.
• W3119043322 cites W1994591844 @default.
• W3119043322 cites W1996604340 @default.
• W3119043322 cites W1998746736 @default.
• W3119043322 cites W2000614648 @default.
• W3119043322 cites W2017189822 @default.
• W3119043322 cites W2021933218 @default.
• W3119043322 cites W2024457806 @default.
• W3119043322 cites W2029948027 @default.
• W3119043322 cites W2031387028 @default.
• W3119043322 cites W2034864366 @default.
• W3119043322 cites W2044939758 @default.
• W3119043322 cites W2047290830 @default.
• W3119043322 cites W2048708082 @default.
• W3119043322 cites W2052821301 @default.
• W3119043322 cites W2065578407 @default.
• W3119043322 cites W2073093837 @default.
• W3119043322 cites W2075115482 @default.
• W3119043322 cites W2077997117 @default.
• W3119043322 cites W2081877006 @default.
• W3119043322 cites W2082864552 @default.
• W3119043322 cites W2086601594 @default.
• W3119043322 cites W2088074390 @default.
• W3119043322 cites W2089831198 @default.
• W3119043322 cites W2107536885 @default.
• W3119043322 cites W2114570899 @default.
• W3119043322 cites W2115326891 @default.
• W3119043322 cites W2118161743 @default.
• W3119043322 cites W2137244077 @default.
• W3119043322 cites W2146061786 @default.
• W3119043322 cites W2149203369 @default.
• W3119043322 cites W2149430024 @default.
• W3119043322 cites W2154443586 @default.
• W3119043322 cites W2157391140 @default.
• W3119043322 cites W2159181928 @default.
• W3119043322 cites W2159958980 @default.
• W3119043322 cites W2161458733 @default.
• W3119043322 cites W2162909305 @default.
• W3119043322 cites W2168059482 @default.
• W3119043322 cites W2176603852 @default.
• W3119043322 cites W2232262725 @default.
• W3119043322 cites W2265915906 @default.
• W3119043322 cites W2301096637 @default.
• W3119043322 cites W2311496691 @default.
• W3119043322 cites W2414767143 @default.
• W3119043322 cites W2518390495 @default.
• W3119043322 cites W2561757389 @default.
• W3119043322 cites W2610169186 @default.
• W3119043322 cites W2768831599 @default.
• W3119043322 cites W2789408575 @default.
• W3119043322 cites W2800731924 @default.
• W3119043322 cites W2889565523 @default.
• W3119043322 cites W2890918208 @default.
• W3119043322 cites W2941787904 @default.
• W3119043322 cites W3119505705 @default.
• W3119043322 cites W3127662229 @default.
• W3119043322 cites W75939939 @default.
• W3119043322 doi "https://doi.org/10.1038/s41598-021-96511-4" @default.
• W3119043322 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8376994" @default.
• W3119043322 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34413433" @default.
• W3119043322 hasPublicationYear "2021" @default.
• W3119043322 type Work @default.
• W3119043322 sameAs 3119043322 @default.
• W3119043322 citedByCount "7" @default.
• W3119043322 countsByYear W31190433222021 @default.
• W3119043322 countsByYear W31190433222022 @default.
• W3119043322 countsByYear W31190433222023 @default.
• W3119043322 crossrefType "journal-article" @default.
• W3119043322 hasAuthorship W3119043322A5020797341 @default.
• W3119043322 hasAuthorship W3119043322A5038911681 @default.
• W3119043322 hasAuthorship W3119043322A5041152613 @default.
• W3119043322 hasAuthorship W3119043322A5060243216 @default.
• W3119043322 hasAuthorship W3119043322A5067022501 @default.
• W3119043322 hasAuthorship W3119043322A5087016011 @default.
• W3119043322 hasAuthorship W3119043322A5089372948 @default.

• next