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- W3119084443 abstract "目的本文旨在研究低氧环境下改变种蛋内铜离子（Cu2+）含量对藏鸡、科宝肉鸡两品种鸡胚发育、血管生成和机体抗氧化性能的影响及机制。 方法选择质量相近的藏鸡、科宝肉鸡种蛋各132枚，随机分为3组。孵化前蛋内注射不同Cu2+浓度的CuSO4注射液100 μL：对照组（仅注射100 μL 90 μg·mL-1 NaCl溶液）、低剂量组（75 μg·mL-1 Cu2+），高剂量组（300 μg·mL-1 Cu2+），低氧环境下孵化。12胚龄时固定和采集尿囊膜，采用Image-Pro Plus 6.0图像分析软件对鸡胚尿囊膜血管面积、条数进行测量；记录出雏率，采集1日龄血清及各组织并测定其质量；使用试剂盒检测与抗氧化相关的指标；采用RT-qPCR检测肝脏和尿囊膜中血管内皮生长因子（VEGF）基因的表达。 结果肉鸡出雏率随蛋内注射Cu2+浓度增加而升高；与对照组相比，低剂量组肉鸡出雏质量/蛋质量、腿体比以及高剂量组肝体比均显著提高（P 结论低氧环境下种蛋内注射CuSO4可提高藏鸡和肉鸡机体的抗氧化能力，促进两品种鸡胚血管生成以应对低氧环境；肉鸡机体抗氧化能力强于藏鸡，而藏鸡的血管生成能力强于肉鸡。" @default.
- W3119084443 created "2021-01-18" @default.
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- W3119084443 date "2020-11-30" @default.
- W3119084443 modified "2023-09-26" @default.
- W3119084443 title "低氧环境下蛋内注射CuSO 4 对藏鸡和肉鸡鸡胚发育的影响" @default.
- W3119084443 doi "https://doi.org/10.7685/jnau.201910038" @default.
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