Matches in SemOpenAlex for { <https://semopenalex.org/work/W3119157170> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W3119157170 endingPage "161" @default.
- W3119157170 startingPage "147" @default.
- W3119157170 abstract "Major Royal Jelly Protein 1 (MRJP1) is the main protein component of the bee-produced complex mixture royal jelly, which is the only nutrient source for queen bees promoting increased lifespan, body size and fertility. Recombinant production of MRJP1 represents an alternative to direct extraction from royal jelly. Production in Pichia pastoris results in high density biomass, with a supernatant containing high amount of impurities. Various methods have been applied to recover and/or purify MRJP1. Here, exploiting the physicochemical properties of MRJP1, reverse phase chromatography (RPC), size exclusion chromatography (SEC) and ion-exchange chromatography (IEX) were investigated as alternative methods to recover MRJP1 directly from supernatant. All techniques showed a 57-kDa band in SDS-PAGE analysis, corresponding to the size of recombinant MRJP1, with contaminants attributed to culture media. However, SEC coupled to IEX evidenced a single peak in the chromatogram corresponding to MRJP1 which suggest it may be a good protocol to recover recombinant MRJP1 from P. pastoris supernatant. This approach serves as a procedure to identify MRJP1 in fermentation culture of P. pastoris. This is the first report about characterization of IEX-based recovery of recombinant Apis mellifera MRJP1 produced in Pichia pastoris without the use of histidine tags." @default.
- W3119157170 created "2021-01-18" @default.
- W3119157170 creator A5010221433 @default.
- W3119157170 creator A5019301408 @default.
- W3119157170 creator A5021140906 @default.
- W3119157170 creator A5065346059 @default.
- W3119157170 creator A5075767956 @default.
- W3119157170 creator A5091447600 @default.
- W3119157170 date "2020-07-27" @default.
- W3119157170 modified "2023-10-04" @default.
- W3119157170 title "Partial recovery of MRJP1 protein expressed in Pichia pastoris using chromatographic techniques" @default.
- W3119157170 doi "https://doi.org/10.24275/rmiq/bio1713" @default.
- W3119157170 hasPublicationYear "2020" @default.
- W3119157170 type Work @default.
- W3119157170 sameAs 3119157170 @default.
- W3119157170 citedByCount "0" @default.
- W3119157170 crossrefType "journal-article" @default.
- W3119157170 hasAuthorship W3119157170A5010221433 @default.
- W3119157170 hasAuthorship W3119157170A5019301408 @default.
- W3119157170 hasAuthorship W3119157170A5021140906 @default.
- W3119157170 hasAuthorship W3119157170A5065346059 @default.
- W3119157170 hasAuthorship W3119157170A5075767956 @default.
- W3119157170 hasAuthorship W3119157170A5091447600 @default.
- W3119157170 hasBestOaLocation W31191571701 @default.
- W3119157170 hasConcept C100544194 @default.
- W3119157170 hasConcept C104317684 @default.
- W3119157170 hasConcept C181199279 @default.
- W3119157170 hasConcept C185592680 @default.
- W3119157170 hasConcept C193363816 @default.
- W3119157170 hasConcept C206967267 @default.
- W3119157170 hasConcept C2779222958 @default.
- W3119157170 hasConcept C2779703480 @default.
- W3119157170 hasConcept C2781372317 @default.
- W3119157170 hasConcept C40767141 @default.
- W3119157170 hasConcept C43617362 @default.
- W3119157170 hasConcept C55493867 @default.
- W3119157170 hasConceptScore W3119157170C100544194 @default.
- W3119157170 hasConceptScore W3119157170C104317684 @default.
- W3119157170 hasConceptScore W3119157170C181199279 @default.
- W3119157170 hasConceptScore W3119157170C185592680 @default.
- W3119157170 hasConceptScore W3119157170C193363816 @default.
- W3119157170 hasConceptScore W3119157170C206967267 @default.
- W3119157170 hasConceptScore W3119157170C2779222958 @default.
- W3119157170 hasConceptScore W3119157170C2779703480 @default.
- W3119157170 hasConceptScore W3119157170C2781372317 @default.
- W3119157170 hasConceptScore W3119157170C40767141 @default.
- W3119157170 hasConceptScore W3119157170C43617362 @default.
- W3119157170 hasConceptScore W3119157170C55493867 @default.
- W3119157170 hasIssue "1" @default.
- W3119157170 hasLocation W31191571701 @default.
- W3119157170 hasOpenAccess W3119157170 @default.
- W3119157170 hasPrimaryLocation W31191571701 @default.
- W3119157170 hasRelatedWork W2019853669 @default.
- W3119157170 hasRelatedWork W2023742154 @default.
- W3119157170 hasRelatedWork W2364681870 @default.
- W3119157170 hasRelatedWork W2375048564 @default.
- W3119157170 hasRelatedWork W2414398983 @default.
- W3119157170 hasRelatedWork W2921453946 @default.
- W3119157170 hasRelatedWork W3211785032 @default.
- W3119157170 hasRelatedWork W4206881933 @default.
- W3119157170 hasRelatedWork W4248988179 @default.
- W3119157170 hasRelatedWork W4298026644 @default.
- W3119157170 hasVolume "20" @default.
- W3119157170 isParatext "false" @default.
- W3119157170 isRetracted "false" @default.
- W3119157170 magId "3119157170" @default.
- W3119157170 workType "article" @default.