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• W3119195422 abstract "The human leukemia cell line (HL-60) is an alternative to primary neutrophils in research studies. However, because HL-60 cells proliferate in an incompletely differentiated state, they must undergo differentiation before they acquire the functional properties of neutrophils. Here we provide evidence of swarming and chemotaxis in differentiated HL-60 neutrophil-like cells (dHL-60) using precise microfluidic assays. We found that dimethyl sulfoxide differentiated HL-60 cells (DdHL-60) have a larger size, increased length, and lower ability to squeeze through narrow channels compared to primary neutrophils. They migrate through tapered microfluidic channels slower than primary neutrophils, but faster than HL-60s differentiated by other protocols, e.g., using all-trans retinoic acid. We found that dHL-60 can swarm toward zymosan particle clusters, though they display disorganized migratory patterns and produce swarms of smaller size compared to primary neutrophils." @default.
• W3119195422 created "2021-01-18" @default.
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• W3119195422 date "2021-01-12" @default.
• W3119195422 modified "2023-10-15" @default.
• W3119195422 title "Chemotaxis and swarming in differentiated HL-60 neutrophil-like cells" @default.
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• W3119195422 doi "https://doi.org/10.1038/s41598-020-78854-6" @default.
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