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- W3119200512 abstract "Fibroblasts isolated from a skin biopsy of a healthy 46-year-old female were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate isogenic cell lines with a gene dose-dependent deficiency of CDH13, a risk gene associated with neurodevelopmental and psychiatric disorders. Thereby, a heterozygous CDH13 knockout ( CDH13 +/− ) and a CDH13 null mutant ( CDH13 −/− ) iPSC line was obtained. All three lines showed expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro , and a normal female karyotype." @default.
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- W3119200512 date "2021-03-01" @default.
- W3119200512 modified "2023-10-11" @default.
- W3119200512 title "Generation of induced pluripotent stem cell (iPSC) lines carrying a heterozygous (UKWMPi002-A-1) and null mutant knockout (UKWMPi002-A-2) of Cadherin 13 associated with neurodevelopmental disorders using CRISPR/Cas9" @default.
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- W3119200512 doi "https://doi.org/10.1016/j.scr.2021.102169" @default.
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