FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3119204206> ?p ?o ?g. }

• W3119204206 endingPage "198" @default.
• W3119204206 startingPage "189" @default.
• W3119204206 abstract "Background. In modern cardiovasclar surgery, it is a promising method to use xenotissues, which in their properties are close to human tissues, in order to restore the integrity of the heart chambers, its walls or valves. Decellularization of extracellular matrix (EMC) is applied in the process of creation of such bioprotheses. In EMC, the elastin and collagen components are preserved, and antigenic molecules are eliminated resulting in reduction the risk of rejection. The study is devoted to assessment of the histological, molecular-genetic and cytotoxic properties of decellularized bovine pericardium, processed with various concentrations of trypsin enzyme.Objective. The aim of the work is evaluation of efficiency of bovine pericardial decellularization, based on the use of trypsin enzyme with 1% Sodium Dodecyl Sulfate (SDS) and 0.1% SDS.Methods. Bovine pericardium was used as a biomaterial for decellularization. Decellularization protocol 1 envisages processing of samples with 0.25% Trypsin solution at 24 °C with constant shaking (200 rpm) along with processing with 1% ionic SDS detergent. The samples, prepared according to protocol 2, were processed with a low concentration of 0.1% SDS. Histological and morphological properties along with detection of nucleic acids concentration in the samples were studied. Matrix samples were cultured in a human fibroblasts cell culture suspension in order to determine cytotoxicity.Results. Histological examination has not revealed any presence of cells in tissues, decellularized in accordance with both protocols. More than 99% of the nucleic acids was removed from decellularized bovine matrix. During our study, we have not observed cytotoxic effect in vitro for protocol 2 matrix samples, decellularized with only 0.1% SDS. Focal destruction of fibroblasts was observed in conditions of long-term cultivation in protocol 1 samples (Trypsin + 1% SDS). Cells formed abnormal morphological aggregates. Samples of this group have also demonstated sructural changes in collagen and elastin fibers.Conclusions. Studies have shown that pericardial matrix tissue, decellularized with low-concentration of 0.1% SDS, has the same biological properties as the native pericardium. Decellularization of bovine pericardium, using trypsin enzyme with 1% SDS, has a cytotoxic effect on human cells." @default.
• W3119204206 created "2021-01-18" @default.
• W3119204206 creator A5003381202 @default.
• W3119204206 creator A5011759629 @default.
• W3119204206 creator A5013089120 @default.
• W3119204206 creator A5041683132 @default.
• W3119204206 creator A5047365089 @default.
• W3119204206 creator A5054582455 @default.
• W3119204206 creator A5056018110 @default.
• W3119204206 creator A5065939751 @default.
• W3119204206 date "2020-12-18" @default.
• W3119204206 modified "2023-09-30" @default.
• W3119204206 title "The Efficiency of Decellularization of Bovine Pericardium by Different Concentrations of Sodium Dodecyl Sulfate" @default.
• W3119204206 cites W1504644673 @default.
• W3119204206 cites W1731768350 @default.
• W3119204206 cites W1975971512 @default.
• W3119204206 cites W1987602789 @default.
• W3119204206 cites W2024316870 @default.
• W3119204206 cites W2052199548 @default.
• W3119204206 cites W2055162535 @default.
• W3119204206 cites W2059486645 @default.
• W3119204206 cites W2062361709 @default.
• W3119204206 cites W2068066669 @default.
• W3119204206 cites W2078246403 @default.
• W3119204206 cites W2109385127 @default.
• W3119204206 cites W2165617122 @default.
• W3119204206 cites W2170738954 @default.
• W3119204206 cites W2171890040 @default.
• W3119204206 cites W2296538351 @default.
• W3119204206 cites W2336452123 @default.
• W3119204206 cites W2488182834 @default.
• W3119204206 cites W2561109298 @default.
• W3119204206 cites W2566945325 @default.
• W3119204206 cites W2743956150 @default.
• W3119204206 cites W2746009463 @default.
• W3119204206 cites W2756476479 @default.
• W3119204206 cites W2765763865 @default.
• W3119204206 cites W2942388658 @default.
• W3119204206 cites W2954342709 @default.
• W3119204206 cites W2990662331 @default.
• W3119204206 cites W3024781239 @default.
• W3119204206 doi "https://doi.org/10.20535/ibb.2020.4.4.214765" @default.
• W3119204206 hasPublicationYear "2020" @default.
• W3119204206 type Work @default.
• W3119204206 sameAs 3119204206 @default.
• W3119204206 citedByCount "1" @default.
• W3119204206 countsByYear W31192042062021 @default.
• W3119204206 crossrefType "journal-article" @default.
• W3119204206 hasAuthorship W3119204206A5003381202 @default.
• W3119204206 hasAuthorship W3119204206A5011759629 @default.
• W3119204206 hasAuthorship W3119204206A5013089120 @default.
• W3119204206 hasAuthorship W3119204206A5041683132 @default.
• W3119204206 hasAuthorship W3119204206A5047365089 @default.
• W3119204206 hasAuthorship W3119204206A5054582455 @default.
• W3119204206 hasAuthorship W3119204206A5056018110 @default.
• W3119204206 hasAuthorship W3119204206A5065939751 @default.
• W3119204206 hasBestOaLocation W31192042061 @default.
• W3119204206 hasConcept C136229726 @default.
• W3119204206 hasConcept C181199279 @default.
• W3119204206 hasConcept C185592680 @default.
• W3119204206 hasConcept C189165786 @default.
• W3119204206 hasConcept C190672674 @default.
• W3119204206 hasConcept C2778913249 @default.
• W3119204206 hasConcept C2780340462 @default.
• W3119204206 hasConcept C55493867 @default.
• W3119204206 hasConcept C71924100 @default.
• W3119204206 hasConceptScore W3119204206C136229726 @default.
• W3119204206 hasConceptScore W3119204206C181199279 @default.
• W3119204206 hasConceptScore W3119204206C185592680 @default.
• W3119204206 hasConceptScore W3119204206C189165786 @default.
• W3119204206 hasConceptScore W3119204206C190672674 @default.
• W3119204206 hasConceptScore W3119204206C2778913249 @default.
• W3119204206 hasConceptScore W3119204206C2780340462 @default.
• W3119204206 hasConceptScore W3119204206C55493867 @default.
• W3119204206 hasConceptScore W3119204206C71924100 @default.
• W3119204206 hasIssue "4" @default.
• W3119204206 hasLocation W31192042061 @default.
• W3119204206 hasLocation W31192042062 @default.
• W3119204206 hasLocation W31192042063 @default.
• W3119204206 hasOpenAccess W3119204206 @default.
• W3119204206 hasPrimaryLocation W31192042061 @default.
• W3119204206 hasRelatedWork W1999528013 @default.
• W3119204206 hasRelatedWork W2594477819 @default.
• W3119204206 hasRelatedWork W2749059833 @default.
• W3119204206 hasRelatedWork W3006202201 @default.
• W3119204206 hasRelatedWork W3025660442 @default.
• W3119204206 hasRelatedWork W3082373304 @default.
• W3119204206 hasRelatedWork W3134878107 @default.
• W3119204206 hasRelatedWork W4223441938 @default.
• W3119204206 hasRelatedWork W4289274994 @default.
• W3119204206 hasRelatedWork W4289275187 @default.
• W3119204206 hasVolume "4" @default.
• W3119204206 isParatext "false" @default.
• W3119204206 isRetracted "false" @default.
• W3119204206 magId "3119204206" @default.
• W3119204206 workType "article" @default.