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- W3119242801 abstract "Mosaic animals have provided the platform for many fundamental discoveries in developmental biology, cell biology, and other fields. Techniques to produce mosaic animals by mitotic recombination have been extensively developed in Drosophila melanogaster but are less common for other laboratory organisms. Here, we report m osaic a nalysis by g RNA- i nduced c rossing-over (MAGIC), a new technique for generating mosaic animals based on DNA double-strand breaks produced by CRISPR/Cas9. MAGIC efficiently produces mosaic clones in both somatic tissues and the germline of Drosophila . Further, by developing a MAGIC toolkit for 1 chromosome arm, we demonstrate the method’s application in characterizing gene function in neural development and in generating fluorescently marked clones in wild-derived Drosophila strains. Eliminating the need to introduce recombinase-recognition sites into the genome, this simple and versatile system simplifies mosaic analysis in Drosophila and can in principle be applied in any organism that is compatible with CRISPR/Cas9." @default.
- W3119242801 created "2021-01-18" @default.
- W3119242801 creator A5011640671 @default.
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- W3119242801 date "2021-01-14" @default.
- W3119242801 modified "2023-10-04" @default.
- W3119242801 title "Versatile CRISPR/Cas9-mediated mosaic analysis by gRNA-induced crossing-over for unmodified genomes" @default.
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- W3119242801 doi "https://doi.org/10.1371/journal.pbio.3001061" @default.
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