Matches in SemOpenAlex for { <https://semopenalex.org/work/W3119568077> ?p ?o ?g. }
- W3119568077 endingPage "1113" @default.
- W3119568077 startingPage "1097" @default.
- W3119568077 abstract "The identification of stallions and or ejaculates that will provide commercially acceptable quality post-thaw before cryopreservation is of great interest, avoiding wasting time and resources freezing ejaculates that will not achieve sufficient quality to be marketed. Our hypothesis was that after bioinformatic analysis, the study of the stallion sperm proteome can provide discriminant variables able to predict the post-thaw quality of the ejaculate. At least three ejaculates from 10 different stallions were frozen following a split sample design. Half of the ejaculate was analyzed as a fresh aliquot and the other half was frozen and then analyzed as a frozen-thawed aliquot. Computer-assisted sperm analysis and flow cytometry were used to analyze sperm quality. Detailed proteomic analysis was performed on fresh and frozen and thawed aliquots, and bioinformatic analysis was used to identify discriminant variables in fresh samples able to predict the outcome of cryopreservation. Those with a fold change > 3, a P = 8.2e-04, and a q = 0.074 (equivalent to False discovery rate (FDR)) were selected, and the following proteins were identified in fresh samples as discriminant variables of good motility post-thaw: F6YTG8, K9K273, A0A3Q2I7V9, F7CE45, F6YU15, and F6SKR3. Other discriminant variables were also identified as predictors of good mitochondrial membrane potential and viability post-thaw. We concluded that proteomic approaches are a powerful tool to improve current sperm biotechnologies." @default.
- W3119568077 created "2021-01-18" @default.
- W3119568077 creator A5009932635 @default.
- W3119568077 creator A5024445965 @default.
- W3119568077 creator A5027761244 @default.
- W3119568077 creator A5028592188 @default.
- W3119568077 creator A5033841655 @default.
- W3119568077 creator A5050817135 @default.
- W3119568077 creator A5091144227 @default.
- W3119568077 date "2021-01-12" @default.
- W3119568077 modified "2023-10-18" @default.
- W3119568077 title "Differences in the proteome of stallion spermatozoa explain stallion-to-stallion variability in sperm quality post-thaw†" @default.
- W3119568077 cites W158461146 @default.
- W3119568077 cites W1969778539 @default.
- W3119568077 cites W1971133517 @default.
- W3119568077 cites W1976820001 @default.
- W3119568077 cites W1981740138 @default.
- W3119568077 cites W1992372128 @default.
- W3119568077 cites W1995098566 @default.
- W3119568077 cites W2000092397 @default.
- W3119568077 cites W2000264054 @default.
- W3119568077 cites W2000507065 @default.
- W3119568077 cites W2011945645 @default.
- W3119568077 cites W2020594289 @default.
- W3119568077 cites W2021119961 @default.
- W3119568077 cites W2022737873 @default.
- W3119568077 cites W2034728682 @default.
- W3119568077 cites W2038562206 @default.
- W3119568077 cites W2044953704 @default.
- W3119568077 cites W2048254682 @default.
- W3119568077 cites W2054936230 @default.
- W3119568077 cites W2062714635 @default.
- W3119568077 cites W2065520693 @default.
- W3119568077 cites W2075376373 @default.
- W3119568077 cites W2094055315 @default.
- W3119568077 cites W2100683912 @default.
- W3119568077 cites W2126744945 @default.
- W3119568077 cites W2129058819 @default.
- W3119568077 cites W2144538400 @default.
- W3119568077 cites W2147871639 @default.
- W3119568077 cites W2266646185 @default.
- W3119568077 cites W2318552449 @default.
- W3119568077 cites W2467615177 @default.
- W3119568077 cites W2516998493 @default.
- W3119568077 cites W2520306567 @default.
- W3119568077 cites W2534473944 @default.
- W3119568077 cites W2551816155 @default.
- W3119568077 cites W2561071079 @default.
- W3119568077 cites W2609823669 @default.
- W3119568077 cites W2762802201 @default.
- W3119568077 cites W2769772146 @default.
- W3119568077 cites W2795880214 @default.
- W3119568077 cites W2809123391 @default.
- W3119568077 cites W2885220299 @default.
- W3119568077 cites W2939234968 @default.
- W3119568077 cites W2945353628 @default.
- W3119568077 cites W2947757970 @default.
- W3119568077 cites W2950635058 @default.
- W3119568077 cites W2955686027 @default.
- W3119568077 cites W2956160046 @default.
- W3119568077 cites W2999139181 @default.
- W3119568077 cites W3014619688 @default.
- W3119568077 cites W3019540915 @default.
- W3119568077 cites W3036471303 @default.
- W3119568077 cites W3079017985 @default.
- W3119568077 cites W406535775 @default.
- W3119568077 cites W73952711 @default.
- W3119568077 cites W2515346540 @default.
- W3119568077 doi "https://doi.org/10.1093/biolre/ioab003" @default.
- W3119568077 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/33438027" @default.
- W3119568077 hasPublicationYear "2021" @default.
- W3119568077 type Work @default.
- W3119568077 sameAs 3119568077 @default.
- W3119568077 citedByCount "15" @default.
- W3119568077 countsByYear W31195680772021 @default.
- W3119568077 countsByYear W31195680772022 @default.
- W3119568077 countsByYear W31195680772023 @default.
- W3119568077 crossrefType "journal-article" @default.
- W3119568077 hasAuthorship W3119568077A5009932635 @default.
- W3119568077 hasAuthorship W3119568077A5024445965 @default.
- W3119568077 hasAuthorship W3119568077A5027761244 @default.
- W3119568077 hasAuthorship W3119568077A5028592188 @default.
- W3119568077 hasAuthorship W3119568077A5033841655 @default.
- W3119568077 hasAuthorship W3119568077A5050817135 @default.
- W3119568077 hasAuthorship W3119568077A5091144227 @default.
- W3119568077 hasBestOaLocation W31195680772 @default.
- W3119568077 hasConcept C105702510 @default.
- W3119568077 hasConcept C16685009 @default.
- W3119568077 hasConcept C179933525 @default.
- W3119568077 hasConcept C196843134 @default.
- W3119568077 hasConcept C2778093475 @default.
- W3119568077 hasConcept C2781087480 @default.
- W3119568077 hasConcept C3020630785 @default.
- W3119568077 hasConcept C54355233 @default.
- W3119568077 hasConcept C71924100 @default.
- W3119568077 hasConcept C86803240 @default.
- W3119568077 hasConceptScore W3119568077C105702510 @default.
- W3119568077 hasConceptScore W3119568077C16685009 @default.